Background: Safety climate which is a subset of organizational climate in the field of safety mirrors the attitude of people in care centers towards safety. In addition to being in connection with diverse parts of the organization’s function, safety climate can also affect the nurse’s performance. Aim: The present study aimed at determining the predicting role of the safety climate in the professional behavior of nurses working in hospitals affiliated to Bushehr University of Medical Sciences. Methods: In this cross sectional study, which is of descriptive analytical type, 595 expert nurses currently working with more than 6 months of working experience in the medical wards of hospitals affiliated to Bushehr University of Medical Sciences entered the study. Data was gathered from the viewpoint of the nurse and professional behavior through a demographic information form and two safety climate questionnaires. Then the data were analyzed by V.19 SPSS which is statistical software. The data analysis was conducted using univariate regression and multivariate linear regression at the significance level of 0.05. Results: Among the diverse domains of the safety climate, the field of nursing education (P= 0.027, ß=0.104), communicating with other nurses (P= 0.027, ß=0.101) and error reporting (P= <0.001, ß= 0.191) were the direct prediction of professional nursing behavior. Also, apart from safety climate, satisfaction of the nursing job had a direct, statistic and significant relationship with professional behavior (P= <0.001, ß= 0.142). Conclusion: Attempts to create a ward, in which the nurses receive the necessary education in an appropriate time and ameliorate their expertise, as well as a climate in which nurses have such a good relationship with their collages that they can easily talk about the possible mistakes and errors, can upgrade nurse’s professional behavior.