Study of the structure and biosynthetic pathway of lignin in stone cells of pear

Cai, Yongping; Li, Guoqing; Nie, J.; Lin, Yi; Nie, Fan; Zhang, Jinyun; Xu, Yongsheng

doi:10.1016/j.scienta.2010.04.029

Cited by 139 publications

(181 citation statements)

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“…The levels of PbCCOMT3 expression between 47 and 63 DAF were higher than those of PbCCOMT1. These results are consistent with the trend observed in pear for lignin and stone cell contents (Cai et al, 2010;Lu et al, 2011), indicating that the two candidate CCOMT genes are probably involved in stone cell development and lignin biosynthesis in DangShan Su pear fruit.…”

Section: Identification and Spatio-temporal Expression Analysis Of Casupporting

confidence: 91%

“…As its fruit is very nutritious and has important medicinal value, this variety is in great demand both at home and abroad. In recent years, the number of stone cells in the fruits of Dangshan Su pears has increased as a result of the deterioration of field environments and field management (Cai et al, 2010;Jin et al, 2013). Stone cells are formed by parenchyma cells following the secondary thickening and lignification of primary walls.…”

Section: Introductionmentioning

confidence: 99%

“…Stone cells are formed by parenchyma cells following the secondary thickening and lignification of primary walls. This acts as a type of sclerenchymatous cells in the pear pulp, which determines the internal quality of pear fruits (Cai et al, 2010;Jin et al, 2013). An increased concentration of stone cells in pear fruit leads to a muricated taste, poor edible quality, and loss of economic value (Choi and Lee, 2013;Zhao et al, 2013;Yan et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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Bioinformatic and expression analysis of the OMT gene family in Pyrus bretschneideri cv. Dangshan Su

Cheng

Xiong

et al. 2016

Genet. Mol. Res.

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ABSTRACT. With high nutritional value in its fruits, Dangshan Su pear has been widely cultivated in China. The stone cell content in fruits is a key factor affecting fruit quality in pear, and the formation of stone cells has been associated with lignin biosynthesis. O-Methyltransferase (OMT) is a key enzyme involved in lignin metabolism within the phenylpropanoid pathway. Here, we screened 26 OMT genes from the Pyrus bretschneideri cv. Dangshan Su genome using the DNATOOLs software. To characterize the OMT gene family in pear, gene structure, chromosomal localization, and conserved motifs of PbOMTs were analyzed. PbOMTs were divided into two categories, type I (designated PbCCOMTs) and type II (designated PbCOMTs), indicating the differentiation of function during evolution. Based on the analysis of multiple sequence alignment, cis-element prediction, and phylogenetic relationships, two candidate genes, PbCCOMT1 and PbCCOMT3, were selected for the analysis of temporal and spatial gene expression in pear. The promoter regions of both PbCCOMT1 and PbCCOMT3 contain regulatory motifs for lignin synthesis. Moreover, the two genes show high similarity and close phylogenetic relationships with CCOMTs in other species. Expression analysis showed that transcript levels of two PbCCOMTs were positively associated with the contents of both stone cells and lignin during the development of pear fruit. These results suggest that PbCCOMT1 and PbCCOMT3 are closely associated with lignin biosynthesis. These findings will help clarify the function of PbOMTs in lignin metabolism and to elucidate the mechanisms underlying stone cell formation in pear.

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Section: Identification and Spatio-temporal Expression Analysis Of Casupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Bioinformatic and expression analysis of the OMT gene family in Pyrus bretschneideri cv. Dangshan Su

Cheng

Xiong

et al. 2016

Genet. Mol. Res.

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“…Low intensity absorption band at region 850 -1200 cm -1 in Ha 1 compared to Ha 2 shows low purity of hemicellulose fraction due to the higher lignin content for Ha 1 compared to Ha 2 . The absoption band at region between 1126 -1032 cm -1 is a typical band for lignin [12]. Higher intensity absorption band at region 1126 -1032 cm -1 in Ha 1 compared to Ha 2 , proved that lignin content was higher in Ha 1 .…”

Section: Monosaccharide Analysis By Hplcmentioning

confidence: 89%

Fractionation of Hemicellulose From Rice Straw by Alkaline Extraction and Ethanol Precipitation

Zulyadi

Saleh

Sarijo

2016

MJAS

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Hemicelluloses were extracted from rice straw using alkaline extraction and ethanol precipitation. The effects of different extraction conditions; temperature (45 -65 °C), pH (4.5 -6.5), and concentrations of NaOH (0.25 -1 M) on the hemicellulose yield were investigated. Two hemicelllulosic fractions namely Ha 1 and Ha 2 were obtained by precipitation of alkaline soluble hemicellulose in 0.2 volumes and 4 volumes ethanol, respectively. The Ha 1 and Ha 2 were further characterized to obtain total sugar, monosaccharides and Klason Lignin content. The highest hemicellulose yield for Ha 1 was 4.00 % obtained at 55 °C and pH 5.5 with 0.25 M of NaOH, whereas the highest hemicellulose yield for Ha 2 was 19.88 % obtained at 55 °C and pH 5.5 with 0.5 M of NaOH. This study revealed that hemicellulose yield is dependent on the pH, temperature and concentration of NaOH in the alkaline extraction.Keywords: hemicelluloses, rice straw, alkaline extraction, ethanol precipitation Abstrak Hemiselulosa daripada hampas batang padi telah diekstrak melalui pengekstrakan beralkali dan pemendakan etanol. Kesan perbezaan kondisi pengekstrakan; suhu (45 -65 °C), pH (4.5 -6.5), dan kepekatan NaOH (0.25 -1 M) ke atas hasil hemiselulosa dikaji. Dua pecahan hemiselulosa, Ha 1 and Ha 2 telah diperolehi melalui pemendakan hemisellulosa larut alkali dalam isipadu etanol 0.2 dan 4 masing-masing. Ha 1 and Ha 2 dicirikan oleh jumlah gula, kandungan monosakarida dan Klason Lignin. Hasil hemiselulosa tertinggi untuk Ha 1 adalah 4.00 % yang diperolehi pada suhu 55 °C, pH 5.5 and 0.25 M NaOH, manakala untuk Ha 2 adalah 19.88 % diperolehi pada suhu 55 °C, pH 5.5 and kepekatan 0.5 M NaOH. Kajian ini membuktikan hasil hemisellulosa bergantung kepada pH, suhu dan kepekatan NaOH dalam pengekstrakan beralkali. Kata kunci: hemiselulosa, hampas batang padi, pengekstrakan beralkali, pemendakan etanol IntroductionThe main cereal crop in the whole wide world is rice, which is being produced in more than 148 million hectares with huge range of ecosystem. The scientific name of paddy is Oryza sativa. It is an important cereal crop that produce a lot of residues which is rice straw [1]. Rice straw is the rice plant stalk that is left as waste product on the field after harvesting of the rice grain [2]. A lot of rice straw are disposed by biomass burning that leads to air pollution.Therefore, a lot of research work has been performed to find ways of utilizing these agricultural waste. Hemicellulose is the second most abundant polysaccharide in nature, representing approximately 20-30% of

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“…Sclereid is an important factor affecting the texture and quality of pear fruit (Cai et al 2010). The brachysclereids or the stone cells of pear fruit are mainly formed by the secondary cell wall thickening and the deposition of lignin (Tao et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Regulation of gibberellin on gene expressions related with the lignin biosynthesis in ‘Wangkumbae’ pear (Pyrus pyrifolia Nakai) fruit

Yang

Zhang

et al. 2014

Plant Growth Regul

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Study of the structure and biosynthetic pathway of lignin in stone cells of pear

Cited by 139 publications

References 15 publications

Bioinformatic and expression analysis of the OMT gene family in Pyrus bretschneideri cv. Dangshan Su

Bioinformatic and expression analysis of the OMT gene family in Pyrus bretschneideri cv. Dangshan Su

Fractionation of Hemicellulose From Rice Straw by Alkaline Extraction and Ethanol Precipitation

Regulation of gibberellin on gene expressions related with the lignin biosynthesis in ‘Wangkumbae’ pear (Pyrus pyrifolia Nakai) fruit

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