Study of the ultrastructure of <i>Enterococcus faecalis</i> and <i>Streptococcus mutans</i> incubated with salivary antimicrobial peptides

Blancas, Blanca; Lanzagorta, María de Lourdes; Jiménez‐García, Luis Felipe; Lara, R J Mora; Molinari, José Luis; Fernández, Ana

doi:10.1002/cre2.430

Cited by 14 publications

(8 citation statements)

References 88 publications

(100 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Intact, plump cell wall, and this was consistent with an earlier report (Blancas et al, 2021) Abbreviations: CS, Chitosan; PLL, polylysine; E. coli, Escherichia coli; E. faecalis, Enterococcus faecalis; P. aeruginosa, Pseudomonas aeruginosa; S. aureus, Staphylococcus aureus; TEM, transmission electron microscopy.…”

Section: E Faecalissupporting

confidence: 91%

Chitosan utilized for bacterial preparation for scanning electron microscopy

Zhang

Yang

et al. 2021

Microscopy Res & Technique

View full text Add to dashboard Cite

Bacterial sample preparation is crucial for its observation by scanning electron microscopy (SEM). However, the current polylysine (PLL) method leads to bacterial morphological changes. To overcome this problem, we employed chitosan (CS) to coat coverslips to prepare bacteria for SEM and compared it with the PLL method. Coverslips coated with 0.025% (w/v) CS showed satisfactory bacterial binding ability. Within 30 min of binding time, the number of bacteria on CS-coated and PLL-coated coverslips exhibited no differences. Four bacteria strains were employed to compare the differences in SEM images between the two methods. Most of the bacteria showed irregular surface or sticky substances after settling on PLL-coated coverslips, while bacteria with clear surface texture were observed on CS-coated coverslips. Transmission electron microscopy (TEM) images showed deformed bacterial envelope on PLLcoated coverslips; meanwhile, similar intact envelope was observed from the bacteria on CS-coated coverslips and the bacteria without any treatment. The TEM results verified the morphological differences of SEM between the two methods. Except for morphology, the length of the rod-shaped bacteria was longer on CS-coated coverslips than that on PLL-coated coverslips, less shrinkage of the sample was observed, and CS could preserve the length of the rod-shaped bacteria better than PLL in its preparation for SEM. It is demonstrated that the low-cost CS could be utilized in bacterial preparation for SEM to acquire preferable images. Bacterial suspension with optical density at 600 nm of about 0.5, deposited on 0.025% CS-coated coverslips for 30 min, and followed by routine fixation, dehydration, and drying are optimal parameters.

show abstract

Section: E Faecalissupporting

confidence: 91%

Chitosan utilized for bacterial preparation for scanning electron microscopy

Zhang

Yang

et al. 2021

Microscopy Res & Technique

View full text Add to dashboard Cite

show abstract

“…The remarkable antimicrobial activitiy of the AgNPs-based extracts suggests its usage as efficective disinfectants in health care yards. Furthermore, similar to others (Yi et al, 2011;Osonga et al, 2019), we demonstrated remakable antimicrobial activity against some oral pathogens such as S. mutants and to a lesser extent against E. faecalis which play a central role in dental carries and root canal infections (Krzyściak et al, 2014;Blancas et al, 2021;Tu et al, 2022). This implays the potential benefits of the AgNPs-based extracts in preventing dental diseases and promoting oral health.…”

Section: Antimicrobial Activitysupporting

confidence: 90%

Characterization, antibacterial, antioxidant, antidiabetic, and anti-inflammatory activities of green synthesized silver nanoparticles using Phragmanthera austroarabica A. G. Mill and J. A. Nyberg extract

Khodeer

Nasr²,

Swidan³

et al. 2023

Front. Microbiol.

View full text Add to dashboard Cite

IntroductionDiabetes mellitus is a chronic metabolic disorder that exhibited great expansion all over the world. It is becoming an epidemic disease adding a major burden to the health care system, particularly in developing countries.MethodsThe plant under investigation in the current study Phragmanthera austroarabica A. G. Mill and J. A. Nyberg is traditionally used in Saudi Arabia for the treatment of diabetes mellitus. The methanolic extract (200 mg/kg) of the plant and pure gallic acid (40 mg/kg), a major metabolite of the plant, as well as their silver nanoparticle formulae (AgNPs) were evaluated for their antidiabetic activity.Results and DiscussionThe results showed a decrease in body fat, obesity, an improvement in lipid profiles, normalization of hyperglycemia, insulin resistance, and hyperinsulinemia, and an improvement in liver tissue structure and function. However, the results obtained from AgNPs for both extract and the pure gallic acid were better in most measured parameters. Additionally, the activity of both the crude extract of the plant and its AgNPs were evaluated against a number of gram-positive, gram-negative bacteria and fungi. Although the activity of the crude extract ranged from moderate to weak or even non-active, the AgNPs of the plant extract clearly enhanced the antimicrobial activity. AgNPs of the extract demonstrated remarkable activity, especially against the Gram-negative pathogens Proteus vulgaris (MIC 2.5 μg/ml) and Pseudomonas aeruginosa (MIC 5 μg/ml). Furthermore, a promising antimicrobial activity was shown against the Gram-positive pathogen Streptococcus mutants (MIC 1.25 μg/ml).

show abstract

“…This study evaluated three bacteria associated with root caries. S. mutans and S. sanguinis are frequently found in root caries, whereas E. faecalis is more commonly associated with endodontic infections [ 30 ]. However, a growing number of studies have observed it in carious lesions, periodontitis, and peri-implantitis [ 31 ].…”

Section: Discussionmentioning

confidence: 99%

The In Vitro Effect of Laser Irradiation (Er:YAG and CO2) and Chemical Reagents (Hydrogen Peroxide, Sodium Hypochlorite, Chlorhexidine, or Sodium Fluoride) Alone or in Combination on Reducing Root Caries Bacteria

Reddy

Deeb

Kitten

et al. 2022

IJMS

View full text Add to dashboard Cite

(1) Lasers have been used for the treatment of dentinal hypersensitivity and bacterial reductions in periodontology. The purpose of this in vitro study was to evaluate the effect of Carbon Dioxide (CO2) and Erbium-doped Yttrium Aluminum Garnet (Er:YAG) lasers with chlorhexidine (CHX), hydrogen peroxide (H2O2), sodium hypochlorite (NaOCl), or sodium fluoride (NaF) on the viability of oral bacteria associated with root caries. (2) Streptococcus mutans, Streptococcus sanguinis, and Enterococcus faecalis were grown in Brain Heart Infusion (BHI) broth, diluted to an OD660 of 0.5, and treated with antiseptics with or without simultaneous irradiation with the Er:YAG and CO2 lasers for 30 s repeated three times. The treatment groups consisted of 1: no treatment, 2: 0.5% H2O2 alone, 3: 0.5% NaOCl alone, 4: 0.12% CHX alone, 5: 2% NaF alone, 6: laser alone, 7: laser with 0.5% H2O2, 8: laser with 0.5% NaOCl, 9: laser with 0.12% CHX, and 10: laser with 2% NaF for both lasers. The microbial viability was determined through plating and viable colonies were counted, converted into CFU/mL, and transformed into log form. The statistical analysis was performed using a two-tailed paired t-test. (3) The use of CO2 and Er:YAG lasers alone failed to show statistically significant antibacterial activity against any of the bacteria. The only effective monotreatment was CHX for S. mutans. The combined treatment of 0.5% NaOCl with Er:YAG produced the greatest reduction in overall viability. (4) The combination of the Er:YAG laser with 0.5% NaOCl resulted in the largest reduction in bacterial survival when compared to monotherapies with antimicrobial solutions or lasers.

show abstract

Study of the ultrastructure of Enterococcus faecalis and Streptococcus mutans incubated with salivary antimicrobial peptides

Cited by 14 publications

References 88 publications

Chitosan utilized for bacterial preparation for scanning electron microscopy

Chitosan utilized for bacterial preparation for scanning electron microscopy

Characterization, antibacterial, antioxidant, antidiabetic, and anti-inflammatory activities of green synthesized silver nanoparticles using Phragmanthera austroarabica A. G. Mill and J. A. Nyberg extract

The In Vitro Effect of Laser Irradiation (Er:YAG and CO2) and Chemical Reagents (Hydrogen Peroxide, Sodium Hypochlorite, Chlorhexidine, or Sodium Fluoride) Alone or in Combination on Reducing Root Caries Bacteria

Contact Info

Product

Resources

About