Study of ultrafiltration of a single and binary protein solution in a thin spiral channel module

Müller, Carolin; Agarwal, Gopal; Melin, T.; Wintgens, Thomas

doi:10.1016/j.memsci.2003.07.020

Cited by 37 publications

(20 citation statements)

References 18 publications

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“…Previously, the separation of lysozyme from myoglobin has been investigated by a few groups. Müller et al [40] reported the maximum selectivity of lysozyme against myoglobin through cellulose acetate membrane around 3 at pH 7, low transmembrane pressure and zero ionic strength of the protein solution. For similar conditions, we have determined the selectivity value of 36 through the 1-bilayer PEI-ALG coated PAN-C membrane.…”

Section: Separation Of Proteinsmentioning

confidence: 99%

Preparation and characterization of polyacrylonitrile membranes modified with polyelectrolyte deposition for separating similar sized proteins

Mahlicli

Altınkaya

Yürekli

2012

Journal of Membrane Science

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a b s t r a c tOne of the challenges faced by ultrafiltration membranes is to separate proteins with a small difference in their molecular weights. Recently, some researchers tried to overcome this problem by using charged membranes. This study examined the use of layer by layer deposition of polyelectrolytes on the chemically-modified polyacyronitrile membrane to increase the selectivity of the ultrafiltration. The membranes were prepared by wet-phase inversion technique and polyethylenimine (PEI) and alginate (ALG) were chosen as cationic and anionic polyelectrolytes for the modification of the surfaces. Sieving coefficient data were obtained with myoglobin and lysozyme as model proteins. The influences of solution pH, ionic strengths of the protein and polyelectrolyte solution and the number of polyelectrolyte bilayers on both selectivity and throughput were investigated. The highest selectivity and throughput were achieved with the 1-bilayer PEI-ALG coated polyacrylonitrile (PAN) membrane. Increasing the number of coating bilayers or the ionic strength of the protein solution or adding salt into the polyelectrolyte coating solution decreased both the maximum selectivity and throughput of the modified membranes.

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Section: Separation Of Proteinsmentioning

confidence: 99%

Preparation and characterization of polyacrylonitrile membranes modified with polyelectrolyte deposition for separating similar sized proteins

Mahlicli

Altınkaya

Yürekli

2012

Journal of Membrane Science

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“…In addition to the classical filtration parameters, the most important parameters governing such a process are the pH value and the ionic strength. Furthermore, the global transport parameters such as permeability, rejection, selectivity, effective diffusion coefficient and solubility of the protein may play an important role [3]. One of the major difficulties with crossflow filtration research is the black box' nature of the equipment.…”

Section: Introductionmentioning

confidence: 99%

“…Müller et al [3] demonstrated the factors influencing a protein filtration process using single and mixed solutions of lysozyme and myoglobin through a hydrophilic 30 kDa molecular weight cut-off (MWCO) cellulose acetate membrane. The proteins were of similar molar mass and size.…”

Section: Introductionmentioning

confidence: 99%

Crossflow Ultrafiltration of Binary Biomolecule Mixture: Analysis of Permeate Flux, Cake Resistance and Sieving Coefficient

Salgın

2007

Chem Eng & Technol

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The aim of this paper is to demonstrate the effects of hydrodynamic conditions on the permeate flux, cake resistance and sieving coefficient in a crossflow ultrafiltration process separating biomolecules of different molecular weights. A binary mixture of L-phenylalanine (L-phe) and lipase was ultrafiltered through a hydrophobic polyether sulphone (PES) membrane with 10 kDa molecular weight cut-off. The changes in permeate flux, cake layer resistance and observed sieving coefficient with different transmembrane pressures (TMP) and crossflow velocities were evaluated. The effect of TMP was examined at two different velocities (0.114 m s -1 and 0.176 m s -1 ) and the effect of velocity was examined at two different TMP (20 kPa and 115 kPa) for the experimental system designed. In the initial stage of the crossflow filtration, it was determined that the TMP was more effective than the velocity. The cake layer resistance increased with increasing TMP and it decreased with increasing velocity for the high TMP value of 115 kPa. A maximum of the observed sieving coefficient was achieved with increasing velocity. An increase in TMP at low inlet velocity (0.114 m s -1 ) affected the observed sieving coefficient positively.

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“…Ghosh and Cui [8] observed greater self-rejection of LYZ at a pH far away from the pI. Müller et al [27] also reported that LYZ forms a positively charged layer at the membrane surface below its pI. The permeate flux was found to be lower for the LYZ experiments compared with the BSA experiments.…”

Section: Protein Filtration Of Lysozymementioning

confidence: 94%

“…Only at pH 4.9 was a slight increase in rejection be observed. At the higher ionic strength, charge-shielding of the proteins results in reduction in the protein effective size [27]. In addition, less self-rejection of LYZ is anticipated in a solution environment where protein charge is shielded.…”

Section: Protein Filtration Of Lysozymementioning

confidence: 99%

Evaluation and comparison of protein ultrafiltration test results: Dead-end stirred cell compared with a cross-flow system

Becht

Malik

Tarleton

2008

Separation and Purification Technology

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• This article was published in the journal, Separation and Purifica- ABSTRACTDead-end stirred cell devices are commonly used in laboratories to characterise ultrafiltration membranes and their separation behaviour. Additionally, protein separation data from such systems are used for process scale-up. Such devices are operated under conditions that are inherently different from those used during the continuous or semi-continuous processing of industrial feed streams. The work presented in this paper compares the rejection behaviour of single protein solutions in both a dead-end stirred cell (SC) device with that for a crossflow system (CF). The effect of ionic strength (20 mM and 100 mM) and solution pH (4.9, 6.0, 7.1, 8.4 and 11.0) on protein filtration (bovine serum albumin (BSA) and lysozyme (LYZ) from buffered aqueous solutions) behaviour has been investigated using polyethersulfone (PES) membranes with a manufacturer specified molecular weight cut-off (MWCO) of 50 kDa. PES membranes were characterised in terms of dextran MWCO using both the SC and the CF systems. The mode of operation resulted in significant observed differences in the resulting dextran solute rejection curves for the two systems. The observed rejection (R obs ) values for a series of dextran standards were consistently found to be lower for the CF system compared with the SC unit suggesting higher wall concentrations (C w ) due to concentration polarisation effects in the CF unit. Protein ultrafiltration studies with the 50 kDa PES membranes highlighted important differences in observed protein rejection behaviour despite operation of the two systems at the same transmembrane pressures (25 kPa). Solution pH was found to have little effect on the rejection of both BSA and LYZ. The solute rejection was found to be more sensitive to ionic strength effects for the SC device both during BSA and LYZ filtration. Convective mass transfer coefficients and hence the true rejection coefficients (R tr ) were calculated for both systems using the stagnant film model to understand the influence of hydrodynamic effects on the ultrafiltration behaviour of the two systems. The magnitude of the Peclet number (Pe) provides a means of comparing hydrodynamic conditions for the two systems and thereby allows differences in observed solute rejection to be better understood.

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Study of ultrafiltration of a single and binary protein solution in a thin spiral channel module

Cited by 37 publications

References 18 publications

Preparation and characterization of polyacrylonitrile membranes modified with polyelectrolyte deposition for separating similar sized proteins

Preparation and characterization of polyacrylonitrile membranes modified with polyelectrolyte deposition for separating similar sized proteins

Crossflow Ultrafiltration of Binary Biomolecule Mixture: Analysis of Permeate Flux, Cake Resistance and Sieving Coefficient

Evaluation and comparison of protein ultrafiltration test results: Dead-end stirred cell compared with a cross-flow system

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