Study of Ultrasonic-Freeze-Thaw-Cycle Assisted Extraction of Polysaccharide and Phycobiliprotein from <i>Gracilaria lemaneiformis</i>

Abstract

Ultrasonic-freeze-thaw-cycle assisted extraction was used to synchronously extract polysaccharide and phycobiliprotein from Gracilaria lemaneiformis. Firstly, different solvents (deionized water, sodium hydroxide, phosphate buffer solution (PBS) and calcium chloride solution) were screened, and PBS (1 mM, pH 6.8) was considered as the best solvent for its OD565 value (0.086±0.0003) and OD595 value (0.471±0.002), which was significantly higher than any other solvents (p&lt;0.05). Moreover, an L9 (3)4 orthogonal test was carried out to optimize extraction conditions, based on the single-factor test. The results indicated that the optimized conditions were fixed as follows: the ratio of solid to liquid 1:25 (w/v), 2 times of freeze-thaw cycles, ultrasound power 320 W and ultrasound time 10 min, which obtained the optimum yield and purity of polysaccharide and phycobiliprotein, 30.54%, 1.36% and 0.296, respectively. As a result, ultrasonic-freeze-thaw-cycle assisted extraction is considered to be an effective, economic and eco-environmental method to extract polysaccharide and phycobiliprotein.

Amylase-assisted extraction and antioxidant activity of polysaccharides from Gracilaria lemaneiformis

“…The difference in optimal reaction conditions could be due to the differences in substrates. Under the optimum reaction conditions, the yield of GPs reached up to 49.15%, which is higher than those of other extraction methods previously reported (Liu et al 2013;You et al 2016;Yang and Zhuang 2011).…”

“…Recently, studies on different methods for polysaccharide extraction from G. lemaneiformis had been developed, such as hot water, ultrasonic waves, ultrasonic-hot water, microwave-assisted extraction and citric acid (Liu et al 2013;You et al 2016;Yang and Zhuang 2011). Nevertheless, the data regarding enzymatic-assisted extraction of polysaccharides from G. lemaneiformis are limited.…”

Extraction of native agar from Gracillaria sp with ultrasonic acceleration at low temperature

“…Penggunaan ultrasonikasi telah banyak dilakukan dan dilaporkan pada berbagai ekstraksi bahan alam, misalnya ekstraksi antioksidan dari daun jenis Folium eucommiae (Liu et al 2010), ekstraksi fikobiliprotein dari Gracilaria lemaneiformis (Liu et al 2013), depolimersisasi karaginan (Uju et al 2018) dan ekstraksi polisakaria bersulfat dari Gracilaria birdiae (Fidelis et al 2014). Sejauh pengetahuan kami, penggunaan ultrasonikasi pada ekstraksi agar tanpa perlakuan alkali (native agar) masih belum banyak dilaporkan.…”

Antioxidant Activity and Biocompatibility of Fructo-Polysaccharides Extracted from a Wild Species of Ornithogalum from Lebanon

“…Different methods are used to extract polysaccharides from plants, including hot water extraction, ultrasound, microwave [ 6 , 7 ], enzymatic hydrolysis, ionic liquids extraction [ 8 ], and freeze-thaw extraction [ 9 ]. The selected extraction method can affect the antioxidant activity of the products.…”