Study of Y-Chromosome Microdeletions in Azoospermic Infertile Males using Multiplex PCR Analysis

Ambulkar, Prafulla S; Pande, S

doi:10.13005/bbra/2639

Cited by 3 publications

(3 citation statements)

References 25 publications

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“…6,7 Studies show highest frequency of deletion was AZFc type. 8,9 In our study it was AZFa type which may be due to small sample size. Double deletion was observed in two subjects; AZFa+c and AZFb+c (Table 3).…”

Section: Discussionmentioning

confidence: 79%

Observational study of Y chromosome microdeletion, EAA markers and non EAA markers in Chhattishgarh

Sinha

Patel²,

Bagde³

et al. 2021

IJOGR

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Genetic factors contribute to 15% of all causes of male infertility. Y chromosome microdeletion is the second most common genetic cause of male infertility. Screening is important for Yq microdeletion as the defect can be transferred to offspring. Aim of our study is to detect the frequency of Y chromosome microdeletion in idiopathic infertile men using both EAA and non EAA markers in central region of India. Forty men from infertility clinic, seeking treatment of infertility were recruited in the study as cases. Thirty normal fertile men of same origin were recruited as controls. Semen analysis was done and cytogenetic normal infertile men were included in the study. Simplex and multiplex PCR methods were used to detect Yq microdeletions. Frequency of deletion was 11/40 (27.5%). Single deletion of AZF a,b,c were 12.5%, 7.5%, 2.5% respectively (). Double deletions of AZF a+c and b+c were 2.5% each (). Two subjects showed deletion for more than one loci. Overall frequency of deletion depends on sample size, no of markers used, inclusion criteria of subjects and geographic location. So, the screening is important for Yq microdeletion as the defect may be inherited to offspring.

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Section: Discussionmentioning

confidence: 79%

Observational study of Y chromosome microdeletion, EAA markers and non EAA markers in Chhattishgarh

Sinha

Patel²,

Bagde³

et al. 2021

IJOGR

View full text Add to dashboard Cite

show abstract

“…Test for microdeletions in the Y chromosome was performed using multiplex PCR for the male patient to determine the presence or absence of two STS sequences in AZFa ( sY84 and sY86 ), three in AZFb ( sY127, sY134s and sY143 ) and four in AZFc (sY254, sY255, sY156, and sY158 ) regions (Ambulkar & Pande, ).…”

Section: Methodsmentioning

confidence: 99%

Rare case of monocentric isochromosome Y with inversion duplication of p arm in patient diagnosed with azoospermia

2019

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A patient presenting with azoospermia was referred for genetic evaluation, and upon karyotyping, he was revealed to have two cell lines—mos46,X,ider(Y)(q10)inv(Y)(p11.3q11.1)/45,X. Further cytogenetic studies such as C banding and fluorescence in situ hybridization were performed, which revealed an inversion duplication of a segment of the Y chromosome; hence, the derivative chromosome contained two SRY genes but only one centromere. Y chromosome microdeletion studies were performed in select STS sequences of AZFa, AZFb and AZFc regions and found to be negative for microdeletions. For such a case of infertility, the couple was advised to undergo artificial reproductive techniques with the help of donor spermatozoa.

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“…There is a major worry that genetic anomalies may be passed down to male offspring, resulting in a more severe phenotype of infertility. The predominance of chromosome factors in infertile male ranges between 2 and 8%, but has recently increased to 15% in azoospermic males (Ambulkar & Pande, 2018). FSH is an important hormone that is required for the initiation of spermatogenesis and must be controlled in infertile men from the therapeutic and clinical intervention standpoint (Oduwole et al, 2018).…”

Section: Genetic Damagementioning

confidence: 99%

Research Advances on the Adverse Effects of Antibiotics on Male Fertility

P.,

R.,

et al. 2023

African Journal of Biology and Medical Research

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With the widespread use of antibiotics, bacterial infection-related morbidity and mortality have significantly declined, revolutionizing modern medicine. However, concerns have been raised concerning the negative effects of antibiotics on a number of physiological systems, including the health of male reproductive systems. The purpose of this in-depth review is to investigate and summarize the body of knowledge about the effects of antibiotics on male fertility. Antibiotics may have negative impacts on male reproductive characteristics, according to a growing body of studies over the past few decades. Several antibiotic groups, including fluoroquinolones, tetracyclines, and sulfonamides, have been linked to altered sperm quality, lower sperm count, impaired sperm motility, altered DNA integrity and morphology of sperm. Male reproductive health is thought to be negatively impacted by antibiotics in a multifactorial manner. It has been suggested that hormonal imbalances, oxidative stress, and disturbances of the delicate male reproductive system's balance may be the underlying mechanisms for the effects that have been observed. Additionally, changes brought on by antibiotics to the gut microbiota, which is known to be extremely important for overall health, may indirectly affect male fertility by altering systemic inflammation and hormone regulation. Additionally, the timing and length of antibiotic exposure appear to be important variables in determining their effect on male fertility, of which there is proof that repetitive or continued drug use may have more severe side effects. The possible negative effects of antibiotics on male fertility are highlighted in this review. Although the available data support a logical relationship between antibiotic usage and male reproductive health, more, well conducted research on humans are still required to clarify the underlying mechanisms and determine the clinical relevance of these results. Future studies in this field might open the door to stronger protections for male fertility while ensuring efficient control of bacterial infections.

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Study of Y-Chromosome Microdeletions in Azoospermic Infertile Males using Multiplex PCR Analysis

Cited by 3 publications

References 25 publications

Observational study of Y chromosome microdeletion, EAA markers and non EAA markers in Chhattishgarh

Observational study of Y chromosome microdeletion, EAA markers and non EAA markers in Chhattishgarh

Rare case of monocentric isochromosome Y with inversion duplication of p arm in patient diagnosed with azoospermia

Research Advances on the Adverse Effects of Antibiotics on Male Fertility

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