Study of ZnS Nanostructures Based Electrochemical and Photoelectrochemical Biosensors for Uric Acid Detection

Abstract

Uric acid (UA) is a kind of purine metabolism product and important in clinical diagnosis. In this work, we present a study of ZnS nanostructures-based electrochemical and photoelectrochemical biosensors for UA detection. Through a simple hydrothermal method and varying the ratio of reaction solvents, we obtained ZnS nanomaterials of one-dimensional to three-dimensional morphologies and they were characterized using field emission scanning electron microscopy (FESEM) and X-ray diffraction (XRD). To fabricate the UA biosensor and study the effect of material morphology on its performance, ZnS nanomaterials were deposited on indium tin oxide (ITO) conducting glass and then coated with uricase by physical absorption. Three kinds of working electrodes were characterized by cyclic voltammetry method. The effect of material morphology on performance of UA detection was investigated via amperometric response based electrochemical method based on enzymatic reaction. The ZnS urchin-like nanostructures electrode shows better sensitivity compared with those made of nanoparticles and nanoflakes because of its high surface-area-to-volume ratio. The photoelectrochemical method for detection of UA was also studied. The sensitivity was increased 5 times after irradiation of 300 nm UV light. These results indicate that ZnS nanostructures are good candidate materials for developing enzyme-based UA biosensors.

Modification of nanocrystalline TiO2 coatings with molecularly imprinted TiO2 for uric acid recognition

“…The detection limit of the UA biosensor was estimated to be about 0.01 μM (S/N = 3). It was obvious that MIT/TiO 2 nanoparticle/QCM electrode exhibited superior linear range and lower detection limit to UA molecules while comparing with the previously reported UA sensors (Table ), which guaranteed its possible applications.…”

“…Over the low concentration range (0.04-45 μM), good linearity was obtained, as illustrated in Figure 6B. (Table 1), [7][8][9]11,12,34,35 which guaranteed its possible applications.…”

“…1,2 Recently, the low UA level in the serum and/or cerebrospinal fluid has been proved to be a key biomarker of Parkinson disease. 3 In the past years, some analytical methods have been developed for UA detection, including highperformance liquid chromatography (HPLC), 4 chemiluminescence, 5 electrochemistry, [6][7][8] enzymatic assay, 9,10 fluorescence, 11,12 surface plasmon resonance, 3 and photoelectrochemistry. 11,12 Despite many advances in UA detection, many of these methods still do not meet the growing demand for more sensitive and selective detection of UA.…”

A Review on Zinc Sulphide Thin Film Fabrication for Various Applications Based on Doping Elements

“…However, the linear range is decreased to 0.01 -0.54 mM, which is to be improved in future work. These results provide a simple and efficient approach to fabricate ZnS based electrochemical and photoelectrochemical biosensors [32].…”

Uricase Based Enzymatic Biosensor for Non‐invasive Detection of Uric Acid by Entrapment in PVA‐SbQ Polymer Matrix

“…For continuous monitoring of UA, electrochemical bio‐sensing offers significant advantages as a non‐invasive method over other approaches such as colorimetric, chemical, spectrophotometric, high pressure liquid chromatography, isotope dilution mass spectrometry and chemiluminescence . Electrochemical biosensing provides a cost‐effective alternative with greater simplicity and improved sensitivity .…”