Study on aerobic degradation of 2,4,6-trinitrotoluene (TNT) using Pseudarthrobacter chlorophenolicus collected from the contaminated site

Lamba, Jyoti; Anand, Shalini; Dutta, Jayanti; Chatterjee, Soumya; Nagar, Shilpi; Celin, S. Mary; Kumar, Pramod

doi:10.1007/s10661-021-08869-7

Cited by 28 publications

(12 citation statements)

References 39 publications

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“…Out of the five start products, which have no apparent connection to DON exposure, products B and E, were able to biotransform the mycotoxin completely, starting from the first enrichment. During the subsequent enrichments of product B, a shift in bacterial community was observed towards mainly the genera Sphingopyxis , Pseudoxanthomonas , Ochrobactrum and Pseudarthrobacter , which are known for their biodegradation capabilities of complex molecules, such as (polycylic) aromatic hydrocarbons [ 62 , 63 , 64 , 65 , 66 , 67 , 68 ], neonicotinoid insecticides [ 69 ], cyanobacterial toxins [ 66 , 70 , 71 ], phthalate esters [ 72 ], polyhydroxyalkanoates [ 73 ], nitrophenols [ 74 ], 2,4,6-trinitrotoluene [ 75 ], etc. The classes to which these genera belong, the α-, γ-proteobacteria and Actinobacteria, are also linked to the bioremediation and biodegradation of other mycotoxins such as aflatoxins, fumonisins, zearalenone and ochratoxins [ 76 , 77 , 78 , 79 ].…”

Section: Discussionmentioning

confidence: 99%

Bacterial Enrichment Cultures Biotransform the Mycotoxin Deoxynivalenol into a Novel Metabolite Toxic to Plant and Porcine Cells

Vanhoutte

Tender

Demeyere

et al. 2021

Toxins

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The mycotoxin deoxynivalenol (DON), produced in wheat, barley and maize by Fusarium graminearum and Fusarium culmorum, is threatening the health of humans and animals. With its worldwide high incidence in food and feed, mitigation strategies are needed to detoxify DON, maintaining the nutritional value and palatability of decontaminated commodities. A promising technique is biological degradation, where microorganisms are used to biotransform mycotoxins into less toxic metabolites. In this study, bacterial enrichment cultures were screened for their DON detoxification potential, where DON and its potential derivatives were monitored. The residual phytotoxicity was determined through a bioassay using the aquatic plant Lemna minor L. Two bacterial enrichment cultures were found to biotransform DON into a still highly toxic metabolite for plants. Furthermore, a cytotoxic effect was observed on the cellular viability of intestinal porcine epithelial cells. Through liquid chromatography high-resolution mass spectrometry analysis, an unknown compound was detected, and tentatively characterized with a molecular weight of 30.0 Da (i.e., CH2O) higher than DON. Metabarcoding of the subsequently enriched bacterial communities revealed a shift towards the genera Sphingopyxis, Pseudoxanthomonas, Ochrobactrum and Pseudarthrobacter. This work describes the discovery of a novel bacterial DON-derived metabolite, toxic to plant and porcine cells.

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Section: Discussionmentioning

confidence: 99%

Bacterial Enrichment Cultures Biotransform the Mycotoxin Deoxynivalenol into a Novel Metabolite Toxic to Plant and Porcine Cells

Vanhoutte

Tender

Demeyere

et al. 2021

Toxins

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“…4). Steady rise in nitrite concentration is indicative of emergence of DNT as the initial intermediate detected at the preliminary phase of the catabolism of TNT (Lamba et al 2021) but at the concluding phase no DNT was noticed that put forward the increasing metabolism and bacterium accelerating potential in degrading the compound ultimately producing toluene. Earlier, diverse researches recorded transformation of DNT to toluene continuing to TCA cycle via toluene cis-dihydrodiol formation (Esteve-Nunez et al 2001; Serrano-González et al 2018) and here in this study also LC-MS peaks con rmed the same (Fig.…”

Section: E Correlation Between Nitrite Nitrate Ammonium and Tnt Degra...mentioning

confidence: 99%

“…Nyanhongo et al 2009; Gűn Gők et al 2019;Lamba et al 2021). The product of direct reduction of two-electrons of the aromatic ring via hydride addition reaction forms dihydride Meisenheimer complex, hydride Meisenheimer complex and protonated tautomers (Serrano-González et al 2018).…”

mentioning

confidence: 99%

Remediation potential of a soil bacterium, Planomicrobium flavidum (S5-TSA-19) for 2,4,6-trinitrotoluene (TNT) i.e., a nitro-aromatic explosive in aquatic medium.

Lamba

Anand

Dutta

et al. 2022

Preprint

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2,4,6-Trinitrotoluene (TNT), a nitro-aromatic explosive commonly used for defence and several non-violent applications is contributing to serious environmental pollution problems including human health. The current study investigated the remediation potential of a native soil isolate i.e., Planomicrobium flavidum (strain S5-TSA-19) against TNT. At a TNT concentration of 120 mg/L with a specified environment, the isolate remained incubated for 30 days in a minimal salt medium (MSM). Regular growth of the isolate, the concentration of TNT, nitrate, nitrite and ammonium ion were evaluated at a particular time during the experiment. Within 168 hours (i.e., 7 days) of incubation, P. flavidum co-metabolically degraded 100% TNT. The biodegradation procedure succeeded the first order kinetics mechanism. Formation of additional metabolites like 2,4-dinitrotoluene (DNT), 2,4-diamino-6-nitrotoluene (2-DANT) and 2-amino-4,6-dinitrotoluene (2-ADNT), were also witnessed. TNT seems to be non-toxic for the isolate, as it reproduced magnificently in explosive presence. For TNT degradation, the nitro-reductase enzyme was observed to be responsible. This is so far the first reporting of Planomicrobium flavidum, efficiently bioremediating TNT i.e., a nitro-aromatic compound via different degradation pathways, leading to the production of simpler as well as less harmful end products. Further, in the near future, at the field scale application Planomicrobium flavidum may be explored for the bioremediation of TNT (i.e., a nitroaromatic- compound) contaminated effluents.

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“…Typically, energetic materials are discarded through open burning/open detonation, or in landfills. Although many disposal technologies (including activated carbon adsorption [ 36 , 37 , 38 ], photocatalysis [ 39 , 40 , 41 ], oxidation [ 42 , 43 ], biodegradation [ 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 ], hydrothermal processing [ 52 ], and electric discharge [ 53 ] etc.) are being explored, no reliable, safe, environmentally sound, and cost-efficient means of disposal are available yet [ 47 , 48 , 54 ].…”

Section: Introductionmentioning

confidence: 99%

“…Although many disposal technologies (including activated carbon adsorption [ 36 , 37 , 38 ], photocatalysis [ 39 , 40 , 41 ], oxidation [ 42 , 43 ], biodegradation [ 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 ], hydrothermal processing [ 52 ], and electric discharge [ 53 ] etc.) are being explored, no reliable, safe, environmentally sound, and cost-efficient means of disposal are available yet [ 47 , 48 , 54 ]. Therefore, the development of new ways of eliminating energetic materials and related toxins from land and water remains among the urgent concerns to improve environmental safety and sustainability.…”

Section: Introductionmentioning

confidence: 99%

Recruiting Perovskites to Degrade Toxic Trinitrotoluene

2021

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Everybody knows TNT, the most widely used explosive material and a universal measure of the destructiveness of explosions. A long history of use and extensive manufacture of toxic TNT leads to the accumulation of these materials in soil and groundwater, which is a significant concern for environmental safety and sustainability. Reliable and cost-efficient technologies for removing or detoxifying TNT from the environment are lacking. Despite the extreme urgency, this remains an outstanding challenge that often goes unnoticed. We report here that highly controlled energy release from explosive molecules can be accomplished rather easily by preparing TNT–perovskite mixtures with a tailored perovskite surface morphology at ambient conditions. These results offer new insight into understanding the sensitivity of high explosives to detonation initiation and enable many novel applications, such as new concepts in harvesting and converting chemical energy, the design of new, improved energetics with tunable characteristics, the development of powerful fuels and miniaturized detonators, and new ways for eliminating toxins from land and water.

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Study on aerobic degradation of 2,4,6-trinitrotoluene (TNT) using Pseudarthrobacter chlorophenolicus collected from the contaminated site

Cited by 28 publications

References 39 publications

Bacterial Enrichment Cultures Biotransform the Mycotoxin Deoxynivalenol into a Novel Metabolite Toxic to Plant and Porcine Cells

Bacterial Enrichment Cultures Biotransform the Mycotoxin Deoxynivalenol into a Novel Metabolite Toxic to Plant and Porcine Cells

Remediation potential of a soil bacterium, Planomicrobium flavidum (S5-TSA-19) for 2,4,6-trinitrotoluene (TNT) i.e., a nitro-aromatic explosive in aquatic medium.

Recruiting Perovskites to Degrade Toxic Trinitrotoluene

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