Study on antibacterial activity and cytocompatibility of Ti–6Al–4V–5Cu alloy

Ma, Zhiqiang; Yao, Mengyu; Liu, R.; Yang, Kè; Ren, Ling; Zhang, Y.; Liao, Zhenhua; Liu, W.; Qi, Min

doi:10.1179/1753555714y.0000000237

Cited by 31 publications

(4 citation statements)

References 29 publications

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“…The ALP activity of GNG Cu/Ti showed the most apparent increase among the three samples, indicating that the differentiation ability of BMSCs on GNG Cu/Ti was greatly enhanced. It has been reported that the Cu 2+ or GNG surface could promote osseointegration, − and our work further clarifies that the GNG surface and Cu 2+ can synergistically promote cell adhering, proliferation, and differentiation.…”

Section: Resultssupporting

confidence: 80%

Enhanced Physicochemical and Biological Properties of a Low-Temperature Copperized Layer on Gradient Nanograined Pure Titanium

Qiu

Pan

Peng

et al. 2021

ACS Appl. Bio Mater.

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The application of titanium as medical implants is limited to a certain extent due to its insufficient corrosion resistance, biological activity, and antibacterial ability. In this work, a gradient nanograined (GNG) layer was fabricated on the titanium surface by surface ultrasonic rolling treatment (SURT). The subsequent copperizing kinetics was greatly enhanced so that a thick copperized layer could be obtained on the surface of GNG Ti at a relatively low diffusion temperature (450 °C). Meanwhile, the GNG structure accelerated the release rate of Cu2+, which endows GNG Cu/Ti with strong antibacterial activity. Moreover, the corrosion resistance and cytocompatibility of GNG Cu/Ti were also evidently improved compared with coarse-grained Ti, indicating a good biomedical application prospect.

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Section: Resultssupporting

confidence: 80%

Enhanced Physicochemical and Biological Properties of a Low-Temperature Copperized Layer on Gradient Nanograined Pure Titanium

Qiu

Pan

Peng

et al. 2021

ACS Appl. Bio Mater.

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“…[25, 26] However, the problem of the falling off of the antibacterial coating cannot be avoided during long-time sustained antibacterial function. In our previous study, [15, 27] a novel class of Cu-bearing titanium alloys, Ti6Al4V5Cu biomaterials with self-antibacterial function, had been successfully designed and fabricated. We found that this Cu-bearing titanium alloy showed not only intense bactericidal effect, but also good cyto-compatibility with the same level of biomedical Ti6Al4V alloy, suggesting its potential application in clinics.…”

Section: Discussionmentioning

confidence: 99%

Toward a Molecular Understanding of the Antibacterial Mechanism of Copper‐Bearing Titanium Alloys against Staphylococcus aureus

Zhu

et al. 2015

Adv Healthcare Materials

152

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The antibacterial mechanism of the Cu-containing materials has not been fully understood although such understanding is crucial for the sustained clinical use of Cu-containing antibacterial materials such as bone implants. The aim of this study is to investigate the molecular mechanisms by which the Gram-positive Staphylococcus aureus (S.aureus) is inactivated through Cu-bearing titanium alloys (Ti6Al4V5Cu). Cu ions released from the alloys were found to contribute to lethal damage of bacteria. They destroyed the permeability of the bacterial membranes, resulting in the leakage of reducing sugars and proteins from the cells. They also promoted the generation of bacteria-killing reactive oxygen species (ROS). The ROS production was confirmed by several assays including fluorescent staining of intracellular oxidative stress, detection of respiratory chain activity, and measurement of the levels of lipid peroxidation, catalase and glutathione. Furthermore, the released Cu ions showed obvious genetic toxicity by interfering the replication of nuc (species-specific) and 16SrRNA genes, but with no effect on the genome integrity. All of these effects lead to the antibacterial effect of Ti6Al4V5Cu. Collectively, our work reconciles the conflicting antibacterial mechanisms of Cu-bearing metallic materials or nanoparticles reported in the literature, as well as highlight the potential use of Ti6Al4V5Cu alloys in inhibiting bacterial infections.

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“…The intermetallic compound on the surface of Ti-Cu alloys enables the Cu-ion to be dissolved from it, thus, get in touch with the bacteria on the surface of alloys [19,50,51]. These Cu-ions can kill bacteria by destroying their cell walls and cell membranes leading to micro-organisms protein solidification and denaturation because the Cu-ion that has strong reduction can extract the electrons from the bacteria, causing their cytoplasm to run off and oxidize their cell nucleus [52][53][54]. Plenty of the intermetallic compounds on the passivation film correspond to more Cu-ions which can be dissolved on the surface of alloys, and makes the samples have better and more stable antibacterial ability.…”

Section: Discussionmentioning

confidence: 99%

Optimization of mechanical properties, biocorrosion properties and antibacterial properties of as-cast Ti–Cu alloys

Zhang

Ren

et al. 2016

Biomed. Mater.

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Ti-Cu sintered alloys have shown good antibacterial abilities. However, the sintered method (powder metallurgy) is not convenient to produce devices with a complex structure. In this paper, Ti-Cu alloys with 2.0, 3.0 and 4.0 wt.% Cu were prepared in an arc melting furnace and subjected to different heat treatments: solid solution and ageing, to explore the possibility of preparing an antibacterial Ti-Cu alloy by a casting method and to examine the effect of Cu content. Phase identification was conducted on an XRD diffraction meter, and the microstructure was observed by a metallographic microscope, a scanning electron microscope (SEM) with energy disperse spectroscopy (EDS) and transmission electron microscopy (TEM). Microhardness and the compressive property of Ti-Cu alloys were tested, and the corrosion resistance and antibacterial activity were assessed in order to investigate the effect of the Cu content. Results showed that the as-cast Ti-Cu alloys exhibited a very low antibacterial rate against Staphylococcus aureus (S. aureus). Heat treatment improved the antibacterial rate significantly, especially after a solid and ageing treatment (T6). Antibacterial rates as high as 90.33% and 92.57% were observed on Ti-3Cu alloy and Ti-4Cu alloy, respectively. The hardness, the compressive yield strength, the anticorrosion resistance and the antibacterial rate of Ti-Cu alloys increased with an increase of Cu content in all conditions. It was demonstrated that homogeneous distribution and a fine TiCu phase played a very important role in the mechanical property, anticorrosion and antibacterial properties. Furthermore, it should be pointed out that the Cu content should be at least 3 wt.% to obtain good antibacterial properties (>90% antibacterial rate) as well as satisfactory mechanical properties.

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Study on antibacterial activity and cytocompatibility of Ti–6Al–4V–5Cu alloy

Cited by 31 publications

References 29 publications

Enhanced Physicochemical and Biological Properties of a Low-Temperature Copperized Layer on Gradient Nanograined Pure Titanium

Enhanced Physicochemical and Biological Properties of a Low-Temperature Copperized Layer on Gradient Nanograined Pure Titanium

Toward a Molecular Understanding of the Antibacterial Mechanism of Copper‐Bearing Titanium Alloys against Staphylococcus aureus

Optimization of mechanical properties, biocorrosion properties and antibacterial properties of as-cast Ti–Cu alloys

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