Study on artificial induction and early development of gynogenetic fat greenling Hexagrammos otakii

Wu, Zhihao; Tan, Xungang; Lijuan, Wang; Song, Zongcheng; Wu, Qiaowan; Wang, Ling; Yu, Zhouxin; Liu, Haoming; You, Feng

doi:10.1016/j.aqrep.2021.100975

“…The fat greenling, an economically important cultured fish species, exhibits distinct seasonal variations in testicular development [19]. During the regression stage of the testis, the main reproductive cells comprise spermatogonia and partially un-ejected sperm [20].…”

Section: Introductionmentioning

confidence: 99%

Immune Rejection Mediated by prf1 and gzmb Affects the Colonization of Fat Greenling (Hexagrammos otakii) Spermatogonia in Heterotransplantation

Zhao,

Chen,

Li

et al. 2024

IJMS

1

0

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Fish germ cell transplantation holds great potential for conserving endangered species, improving cultured fish breeds, and exploring reproductive techniques. However, low transplantation efficiency is a common issue in heterotransplantation. This study transplanted fat greenling (Hexagrammos otakii) spermatogonia into the testes of spotted sea bass (Lateolabrax maculatus) to investigate factors that might affect the colonization and fixation of heterologous transplanted germ cells. Results indicated that transplanted fat greenling spermatogonia cells were successfully detected in the early transplantation phase in spotted sea bass. Their numbers gradually decreased over time, and after 10 days post-transplantation, more than 90% of the transplanted cells underwent apoptosis. Transcriptome sequencing analysis of the testes of spotted sea bass and fat greenling spermatogonia on days 1 and 10 post-transplantation revealed that this apoptosis process involved many immune-related genes and their associated signaling pathways. Acute immune rejection marker genes prf1 and gzmb were detected in the spotted sea bass testes, while immune tolerance genes lck and zap-70 were expressed in the fat greenling spermatogonia. Additionally, differential expression of prf1 and gzmb genes was screened from spotted sea bass, with experimental evidence indicating that PRF1 and GZMB protein from spotted sea bass primarily induce apoptosis in transplanted fat greenling spermatogonia via the mitochondrial apoptosis pathway, at the protein level. This suggests that the difficulties in heterotransplantation are primarily related to acute immune rejection, with PRF1 and GZMB playing significant roles.

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“…The Fat Greenling is a commercially important species due to its high meat quality and rich protein. Fat Greenling are primarily distributed in the Korean Peninsula, Japan, China, and Russia (Wu et al 2022). At present, studies of Fat Greenling have mainly investigated environmental stress, toxicology, and disease (Oh et al 2015; Yang et al 2019; Diao et al 2020).…”

Section: Introductionmentioning

confidence: 99%

Effect of replacing fish meal with chicken gut meal on growth, immune parameters, and metabolism of juvenile Fat Greenling Hexagrammos otakii

Gu,

Peng,

Sun

et al. 2023

N American J Aquac

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ObjectiveChicken gut meal (CGM) is reasonably priced and rich in nutrients and can become a new source of protein in the diets of major aquatic products, such as fish and shrimp.MethodsAn 8‐week feeding trial was conducted to comprehensively investigate the beneficial effects of CGM on juvenile Fat Greenling Hexagrammos otakii. Juveniles (1.58 ± 0.06 g [mean ± SE]) were fed with different levels of CGM in replacement of fish meal (FM) in the basal diet: 0% (CGM0), 25% (CGM25), 50% (CGM50), 75% (CGM75), and 100% (CGM100) replacement, representing a dietary CGM level of up to 40 g/kg.ResultThe results showed that the percent weight gain; specific growth rate; feeding rate; feed conversion ratio; hepatosomatic index; and apparent digestibility coefficients of dry matter, protein, and lipid were significantly improved among the experimental groups. Moreover, the activities of superoxide dismutase and catalase were significantly increased in the CGM25 and CGM50 groups, respectively. The activities of acid phosphatase and alkaline phosphatase were significantly decreased in the CGM75 and CGM100 groups. Triglycerides and total cholesterol were significantly increased in the CGM50 and CGM75 groups, respectively. Fish in the CGM100 and CGM75 groups had significantly higher activities of aspartate aminotransferase and alanine aminotransferase than the control. Moreover, the relative expression levels of the fatty acid synthase and acetyl‐coenzyme A carboxylase alpha genes were significantly increased in the CGM75 and CGM100 groups, while the gene expression levels of hormone‐sensitive lipase and peroxisome proliferator‐activated receptor alpha were significantly decreased.ConclusionIn summary, the optimal replacement level of FM with CGM was shown by curve equation analysis to be 61.30%, and their dosages were 24.52 and 15.48 g/kg, respectively.

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“…H. otakii is mainly distributed in the Korean Peninsula, Japan, China, and Russia. H. otakii is an important commercial variety due to its excellent meat quality, rich protein, and nutrition [21]. However, due to higher price and increasing demand of the farming of H. otakii, fish meal (FM) is compelled to search alternative protein sources.…”

Section: Introductionmentioning

confidence: 99%

Dietary Cinnamaldehyde Enhances Growth Performance, Digestion, Immunity, and Lipid Metabolism in Juvenile Fat Greenling (Hexagrammos otakii)

Gu

¹

,

Han

²

,

Wang

³

et al. 2022

Aquaculture Nutrition

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Fat greenling (Hexagrammos otakii) is a kind of economic fish that is widely consumed by human, and its intensive farming technology is making important progress. However, high-density farming may cause the occurrence of diseases in H. otakii. Cinnamaldehyde (CNE) is a new feed additive for aquatic animals and has a positive effect on disease resistance. In the study, dietary CNE was evaluated on the growth performance, digestion, immune response, and lipid metabolism of juvenile H. otakii ( 6.21 ± 0.19 g). Six experimental diets were formulated containing CNE at levels of 0, 200, 400, 600, 800, and 1000 mg/kg for 8 weeks. The percent weight gain (PWG), specific growth rate (SGR), survival (SR), and feeding rate (FR) were significantly increased by including CNE in fish diets regardless of the inclusion level ( P < 0.05 ). The feed conversion ratio (FCR) was significantly decreased among the groups fed CNE supplemented diets ( P < 0.05 ). A significant decrease in hepatosomatic index (HSI) was observed in fish fed 400 mg/kg-1000 mg/kg CNE compared to the control diet ( P < 0.05 ). Fish-fed diets containing 400 mg/kg and 600 mg/kg CNE had a higher level of crude protein in muscles than the control diet ( P < 0.05 ). Moreover, the activities of lipase (LPS) and pepsin (PEP) in the intestinal were markedly increased in juvenile H. otakii-fed dietary CNE ( P < 0.05 ). Apparent digestibility coefficient (ADC) of dry matter, protein, and lipid was significantly increased with CNE supplement ( P < 0.05 ). The activities of catalase (CAT) and acid phosphatase (ACP) in the liver were markedly enhanced by including CNE in juvenile H. otakii diets compared with the control ( P < 0.05 ). The activities of superoxide dismutase (SOD) and alkaline phosphatase (AKP) in the liver were markedly enhanced in juvenile H. otakii treated with CNE supplements 400 mg/kg-1000 mg/kg ( P < 0.05 ). Additionally, the levels of total protein (TP) in the serum were markedly increased by including CNE in juvenile H. otakii diets compared with the control ( P < 0.05 ). In the CNE200, CNE400, and CNE600 groups, albumin (ALB) levels in the serum were markedly higher compared with that in the control ( P < 0.05 ). In the CNE200 and CNE400 groups, the levels of immunoglobulin G (IgG) in the serum were significantly increased compared with that the control group ( P < 0.05 ). The juvenile H. otakii-fed dietary CNE had lower triglycerides (TG) and total cholesterol (TCHO) levels in the serum than fish-fed CNE-free diets ( P < 0.05 ). The gene expression of peroxisome proliferator-activated receptor alpha (PPAR-α), hormone-sensitive lipase (HSL), and carnitine O-palmitoyltransferase 1 (CPT1) in the liver was significantly increased by including CNE in fish diets regardless of the inclusion level ( P < 0.05 ). However, fatty acid synthase (FAS), peroxisome proliferator-activated receptor gamma (PPAR-γ), and acetyl-CoA carboxylase alpha (ACCα) in the liver were markedly decreased with CNE supplements 400 mg/kg-1000 mg/kg ( P < 0.05 ). The glucose-6-phosphate1-dehydrogenase (G6PD) gene expression levels in the liver were markedly decreased compared with the control ( P < 0.05 ). The optimal supplementation level of CNE was shown by curve equation analysis to be 590.90 mg/kg.

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Study on artificial induction and early development of gynogenetic fat greenling Hexagrammos otakii

Cited by 4 publications

References 34 publications

Immune Rejection Mediated by prf1 and gzmb Affects the Colonization of Fat Greenling (Hexagrammos otakii) Spermatogonia in Heterotransplantation

Immune Rejection Mediated by prf1 and gzmb Affects the Colonization of Fat Greenling (Hexagrammos otakii) Spermatogonia in Heterotransplantation

Effect of replacing fish meal with chicken gut meal on growth, immune parameters, and metabolism of juvenile Fat Greenling Hexagrammos otakii

Dietary Cinnamaldehyde Enhances Growth Performance, Digestion, Immunity, and Lipid Metabolism in Juvenile Fat Greenling (Hexagrammos otakii)

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