Study on Cytotoxicity of “Dextran-Magnetic Layered Double Hydroxide–Fluorouracil” Targeted Liposomes <i>In Vitro</i>

Abstract

This paper studied the cytotoxicity of the dextran-magnetic layered double hydroxide-fluorouracil targeted liposomes ( DET-MLDH-FU liposomes, DMFL ) by MTT method against human ovarian adenocarcinoma cancer cells ( SKOV3 )in vitro. The results shown that the cellular inhibition rate at 24 h increased with the increasing of the fluorouracil concentration in DMFL, DET-MLDH-FU ( DMF ), fluorouracil-loaded liposomes ( FUL ) and free fluorouracil ( FU ). The order of inhibition rate was DMFL, DMF, FUL and FU at same concentration and intervention time. The IC50values of DMFL, DMF, FUL and FU were 14.39, 25.47, 34.39, 48.71 ( μgmL-1), respectively. The results of cytotoxicity experiment proved that the DMFL was superior than other preparations of fluorouracil.

Preparation and drug delivery of dextran-drug complex

“…Because this kind of conjugate has a large molecular volume and cannot enter the blood through the capillary wall, it can only be taken up by the macrophage or reticular epithelial cells through the pinocytosis, and the conjugate is taken into the whole body. Then, under the action of the enzyme, the pharmaceutically active substance is hydrolyzed, thereby killing the cancer cells (Huang, 2013). Therefore, such polymeric carrier-targeted drugs are most suitable for the treatment of lymph node tumors or lymphatic metastasis tumors, and the carrier itself is not antigenic.…”