Synthesis pretreatment and characterization of a magnetic layered double hydroxides fluorescent probe

Dong, Li-E.; Gou, Guojing; Jin, Xin; Zhang, Min

doi:10.1016/j.cclet.2014.03.006

Cited by 3 publications

(3 citation statements)

References 23 publications

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“…3) However, the reason for significant lethal effect of DMF may be its strong transporting and targeting effect compared to 5-Fu. 6) In order to determine the appropriate dose of drug intervention to collect relevant biochemical indicators, this paper firstly implemented a study on the dose-dependent manner and time-dependent manner of 5-Fu and DMF intervention in MGC-803, respectively. 5-Fu and DMF can inhibited cell proliferation of MGC-803 in a concentration-dependent manner after 24 h. The imparity of cell viability stimulated by 5-Fu was 6.28-10.76% between the maximum and the minimum concentration, while the DMF made a difference of 52.95-54.92% (Fig.…”

Section: Dmf Exert Potent Effect In Suppressing Proliferation Againstmentioning

confidence: 99%

“…Our results reflected that the particularity (anti-cancer benefits) of the DMF preparation was a combination effect of 5-Fu and delivery system (MLDH) rather than the simple weighting of 5-Fu and carrier cytotoxicity. 6) DMF Caused Obvious Morphological Characteristics of Distension Death in MGC-803 Cell morphology is one of the most direct ways to identify the type of cell death. The cell morphology was intact, and the mitochondria and endoplasmic reticulum were clear in the control group.…”

Section: Dmf Exert Potent Effect In Suppressing Proliferation Againstmentioning

confidence: 99%

“…Based on MLDH, a supramolecular drug delivery system of "dextran-magnetic layered double hydroxidefluorouracil" (DMF) was developed. [3][4][5][6] DMF has excellent cell transport properties and obvious anti-tumor effects. It can be seen from the living cell imaging in vitro that the cells undergo expansion and rupture in MGC-803 stimulated by DMF, and the cell contents lost eventually, 5) those are consistent with the characteristics of oncosis.…”

Section: Introductionmentioning

confidence: 99%

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The Cell Death Phenotype of MGC-803 Cells Inducing with “Dextran-Magnetic Layered Double Hydroxide-Fluorouracil” Drug Delivery System and Fluorouracil

Pei

Wang

Shu

et al. 2019

Biological & Pharmaceutical Bulletin

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The purpose of the paper is to study the differences in cell death mechanism of MGC-803 induced by "dextran-magnetic layered double hydroxide-fluorouracil" (DMF) drug delivery system and 5-Fluorouracil (5-Fu), respectively. The inhibitory effect on the proliferation was detected via CCK-8. The morphology of cell death was detected by transmission electron microscopy (TEM). Intracellular ATP, mitochondrial membrane potential (MMP), reactive oxygen species (ROS) and Cytosolic Free Ca (Ca 2) level were detected via some methods. The result showed that DMF had more obvious effect in suppressing proliferation compared with 5-Fu, and changed cell death pattern of 5-Fu from apoptosis to oncosis. The ATP decrease, MMP loss, Ca 2 increase, the activation of uncoupling protein-2 (UCP-2) and calpain-1 were significant after DMF exposure. However, DMF did not result in ROS accumulation. DMF could involve in activation of porimin, and the cascade reaction of caspases-3,-7,-9, and-12 and poly ADP-ribose polymerase (PARP) through Western blot. DMF showed a stronger injury on nuclear membrane in the cascade reaction of caspases-6,-8 and lamin-A. DMF triggered rapid depletion of ATP, which was consistent with the phenotype of oncosis. Endogenous mitochondrial apoptosis might not be the main cause of cell swelling. DMF could induce strong endoplasmic reticulum stress (ERS) effect, there might be some signaling pathways related with ERS during the process of oncosis. The calpain system might not be a key factor for structural damage in oncosis induced by DMF. DMF could induce the caspases cascade reactions similar to apoptosis, but inflicted a more strong damage on nuclear membrane and PARP.

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Section: Dmf Exert Potent Effect In Suppressing Proliferation Againstmentioning

confidence: 99%

Section: Dmf Exert Potent Effect In Suppressing Proliferation Againstmentioning

confidence: 99%