Study on genetic variation of Short Tandem Repeats (STR) markers and their association with Somatic Cell Scores (SCS) in crossbred cows

Gupta, Jay Prakash; Bhushan, Bharat; Panigrahi, Manjit; Ranjan, Sanjeev; Asaf, Muhasin; Kumar, Amod; Sulabh, Sourabh; Kumar, Amit; Kumar, Pushpendra; Sharma, Deepak

doi:10.18805/ijar.6709

Cited by 7 publications

(6 citation statements)

References 14 publications

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“…On the same line with the help of PCR-SSCP, Gupta et al . [ 55 ] reported a non-synonymous SNP in exon two of CD14 gene and out of three genotype observed BB genotype was found to be more susceptible to mastitis.…”

Section: Immuno-pathogenesismentioning

confidence: 99%

Molecular markers for resistance against infectious diseases of economic importance

et al. 2017

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Huge livestock population of India is under threat by a large number of endemic infectious (bacterial, viral, and parasitic) diseases. These diseases are associated with high rates of morbidity and mortality, particularly in exotic and crossbred cattle. Beside morbidity and mortality, economic losses by these diseases occur through reduced fertility, production losses, etc. Some of the major infectious diseases which have great economic impact on Indian dairy industries are tuberculosis (TB), Johne’s disease (JD), mastitis, tick and tick-borne diseases (TTBDs), foot and mouth disease, etc. The development of effective strategies for the assessment and control of infectious diseases requires a better understanding of pathogen biology, host immune response, and diseases pathogenesis as well as the identification of the associated biomarkers. Indigenous cattle (Bos indicus) are reported to be comparatively less affected than exotic and crossbred cattle. However, genetic basis of resistance in indigenous cattle is not well documented. The association studies of few of the genes associated with various diseases, namely, solute carrier family 11 member 1, Toll-like receptors 1, with TB; Caspase associated recruitment domain 15, SP110 with JD; CACNA2D1, CD14 with mastitis and interferon gamma, BoLA-DRB3.2 alleles with TTBDs, etc., are presented. Breeding for genetic resistance is one of the promising ways to control the infectious diseases. High host resistance is the most important method for controlling such diseases, but till today no breed is total immune. Therefore, work may be undertaken under the hypothesis that the different susceptibility to these diseases are exhibited by indigenous and crossbred cattle is due to breed-specific differences in the dealing of infected cells with other immune cells, which ultimately influence the immune response responded against infections. Achieving maximum resistance to these diseases is the ultimate goal, is technically possible to achieve, and is permanent. Progress could be enhanced through introgression of resistance genes to breeds with low resistance. The quest for knowledge of the genetic basis for infectious diseases in indigenous livestock is strongly warranted.

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Section: Immuno-pathogenesismentioning

confidence: 99%

Molecular markers for resistance against infectious diseases of economic importance

et al. 2017

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“…Based on earlier researches on microsatellite relationships with mastitis in cows (Hai-Guo et al, 2003;Chu et al, 2005;Gupta et al, 2016), three microsatellites (BM1258, BM1443 and BM1818) were used in this study. Primers sequences used according to cattle genome linkage map.…”

Section: Microsatellite Analysismentioning

confidence: 99%

“…Comparison of individual 18 genotypes of these three microsatellite markers were observed in Table (4 Chu et al (2005) studied the genetic variation of seven microsatellite loci in Beijing Holstein cows which were closely linked to Somatic Cell Score (SCS) and found a significant association with the SCS. Also, Gupta et al (2016) analyzed the association between five microsatellite loci and SCS in 76 Indian crossbred cows. They reported that BM1818 marker not associated with SCS in studied population.…”

Section: Effect Of Genotypes At Microsatellite Loci On Mastitis Incidencementioning

confidence: 99%

Single Marker Association with Mastitis Incidence of Three Microsatellite Loci, BM1258, BM1443 and BM1818, in Egyptian Buffalo

Ghazy

2021

Journal of Animal, Poultry & Fish Production

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To detect the linkage between single markers of microsatellite type and mastitis incidence in Egyptian buffalo three markers (BM1258, BM1443 and BM1818) and one hundred twenty-three lactating animals were used. The selected animals were tested using Modified White Side Test (MWT) to screen animals for mastitis. Non-denatured polyacrylamide gel was used to determine the sizes of the PCR amplified products using reference animal to verify the allelic sizes obtained. For each marker, the frequencies of alleles and genotypes for both positive and negative animal groups were compared by using the chi-square test and the Fisher's exact test. The odds ratios calculated as an estimate of relative risk of mastitis incidence associated with each microsatellite genotypes. A positive mastitis test reaction (MWT) was revealed in 19.5% of total samples. For BM1258, BM1443, and BM1818, the number of alleles was found to be 4, 3 and 5, respectively. The polymorphism in all three studied loci was high (PIC>0.5). The genetic parameters of these loci, including observed and expected heterozygosity, were estimated using full characterizations of this set of three polymorphic loci. The polymorphic information content, heterozygosity, and number of successful alleles of the studied loci showed that BM1443 had the lowest variability and BM1818 had the highest variability, with 0.597 and 0.757, respectively. The overall effects of the three studied markers on mastitis incidence were significantly (P<0.05) different. The genotypes combination '72/79 ', '74/74', and '79/82' at BM1258 loci; '78/78' and '88/88' at BM1443 loci, were observed only in the mastitis free animals. On the other hand, BM4505 loci genotypes were found in the positive populations except the combination of genotypes 134/134, 134/140 and 134/144 animals. The information observed in the present study, could be valuable for improving mastitis resistance in Egyptian buffalo breeds through using molecular tools.

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“…Microsatellites are employed in animals to assess genetic variance within breeds (Kunene et al, 2014). There is also a link between the genetic marker DIK20 and mastitis, as it is a sign of the presence of mastitis in dairy cattle (Gupta et al, 2016). Ajafar et al(2022) reviewed the association of bone morphogenetic protein 15 and growth differentiation factor 9 with litter size in livestock, indicating the potential of these genetic markers in improving fertility and productivity traits in livestock, suggesting that genetic polymorphisms related to fertility traits may have implications for overall productivity in sheep and goats.…”

Section: Introductionmentioning

confidence: 99%

The Relationship Between the Genetic Marker DIK20 and A Range of Physiological and Productive Traits in Holstein Cattle

Awad Hassooni,

Sabbar Atiyah,

Saleh Jassim

2024

JAHP

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The use of gene markers in livestock breeding plays a crucial role in enhancing productivity. Specific genetic markers can be identified, facilitating the task of breeders in selecting desired traits, such as milk production, disease resistance, and growth monitoring, which in turn leads to improving the overall productivity of animals. This research aims to study the linkage among the genetic marker DIK20 and several physiological and productive features in Holstein cattles, which includes milk production, mastitis, and percentages of fat, lactose, and protein. The research was conducted in one milk production season, starting from October 2022 to April 2023, using 60 Holstein cows aged between 4-6 years. The results showed that cows had two genotypes of DIK20, with sizes of 190/181 and 180/170 base pairs, with distribution rates of 36.21 and 63.79%, respectively. Results obzerved highly significant differences (P≤0.01) between the genetic variants. The study also found significant differences (P≤0.05) between the genetic polymorphisms 180/170 and 190/181 in the California mastitis test. However, no statistically significant differences noticed in the genetic polymorphisms related to fat, protein, lactose, and solid non-fat in milk elements, with percentages of 3.60% and 3.48% for fat, 2.90% and 3.1% for protein, 4.18% and 4.31% for lactose, and 7.94% and 8.26% for solid non-fat for the two genetic polymorphisms 180/170 and 190/181, respectively. It is concluded that there is a correlation between the genetic marker DIK20 and various physiological and productive traits in Holstein cows.

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Study on genetic variation of Short Tandem Repeats (STR) markers and their association with Somatic Cell Scores (SCS) in crossbred cows

Cited by 7 publications

References 14 publications

Molecular markers for resistance against infectious diseases of economic importance

Molecular markers for resistance against infectious diseases of economic importance

Single Marker Association with Mastitis Incidence of Three Microsatellite Loci, BM1258, BM1443 and BM1818, in Egyptian Buffalo

The Relationship Between the Genetic Marker DIK20 and A Range of Physiological and Productive Traits in Holstein Cattle

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