Study on iNOS expression and levels of Malondialdehyde in the White Rat (Rattus norvegicus) Exposed with Aggregatibacter actinomycetemcomitans

This paper reports the effect of anthocyanin from purple sweet potato in the cerebellum of ischemic stroke rats model by Middle Cerebral Artery Occlusion (MCAO) technique. This technique was carried out by ligating the blood flow in External Carotid Artery (ECA), Common Carotid Artery (CCA) and Internal Carotid Artery (ICA) for 3 hours, followed by reperfusion. The MCAO technique proved a technique for preparing ischemic stroke rats. This technique induces releasing of Brain-Derived Nuclear Factor (BDNF) and Vascular Endothelial Growth Factor (VEGF) in the cerebellum of ischemic stroke rats. The levels of BDNF measured by ELISA technique and VEGF expression used immunohistochemistry technique. The results showed that anthocyanin from purple sweet potato increases the level of both BDNF and VEGF expression in the cerebellum of ischemic stroke rats. It is suggested that anthocyanin could be used as a therapeutic agent for ischemic stroke.

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“…The sample was taken and dried. The precipitate was mixed with Tris-HCl pH 6,8 with the ratio 1:1 (w/v) [18].…”

Section: Isolations Of Cerebellum Proteinmentioning

confidence: 99%

Potential of Anthocyanin From Purple Sweet Potato (Ipomoea batatas) To Increase BDNF Level and VEGF Expression in The Cerebellum of Ischemic Stroke Rats

Wati

Prasetyawan

Mahdi

et al. 2018

J. Pure App. Chem. Res.

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“…TBA from Sigma Aldrich. Spectrophotometric determination of MDA-TBA withUV-Vis spectrophotometer at λ 533 nm at room temperature, the principal measurement refers to Setin et al [10]. AI was calculated by dividing LDLc with HDLc level.…”

Section: Techniques Of Measurementmentioning

confidence: 99%

The Potential of Rambutan Seed Extract to Reduce Risk of Cardiovascular Disease in Diabetes Mellitus Type 2

Susilowati¹,

Ibrizah²,

Andriani³

et al. 2018

Proceedings of the 5th International Conference on Community Development (AMCA 2018)

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This study aimed to find out the potential of ethanol extract in rambutan seed to reduce the risk factors of cardiovascular disease by using animal model on diabetes-type 2 (DM-2). The animals used for this study were 3-4 months old male wistar mice with 20-25 grams weight. The diabetes mellitus induction type 2 to the animal model was performed through the administration of high fat diet for 4 weeks followed by the daily dosage of Streptozotocin (STZ) 40 mg/kgbw and it was repeated for 4 times in the last week of induction. The administration of 70% ethanol extract of Binjai rambutan seed (Naphelium lappaceum L.) was done in Carboxy Methyl Cellulose 0.5% and metformin as positive control. The result indicated that 70% ethanol extract of Binjai rambutan seeds had a significant effect on the atherosclerosis index (p <0,01) and all lipid profile parameters. The Binjai rambutan seed extract at 19.2 mg/kgbw dose was the most effective to decrease the atherosclerosis index, total cholesterol, triglyceride, low density lipoprotein, and malodialdehyde and to increase high density lipoprotein and super oxide dismutase of mice blood serum. It can be concluded that the rambutan seed extract at the dose of 19.2 mg/kgbw had the potential to reduce the risk of cardiovascular diabetes in mice by increasing lipid fraction and decreasing oxidative stress. Keywords: atherosclerotic index, binjai rambutan seed (Naphelium lappecium L.), diabetes mellitus, lipid profile5th International Conference on Community Development (AMCA 2018)

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Study on iNOS expression and levels of Malondialdehyde in the White Rat (Rattus norvegicus) Exposed with Aggregatibacter actinomycetemcomitans

Cited by 2 publications

References 6 publications

Potential of Anthocyanin From Purple Sweet Potato (Ipomoea batatas) To Increase BDNF Level and VEGF Expression in The Cerebellum of Ischemic Stroke Rats

Potential of Anthocyanin From Purple Sweet Potato (Ipomoea batatas) To Increase BDNF Level and VEGF Expression in The Cerebellum of Ischemic Stroke Rats

The Potential of Rambutan Seed Extract to Reduce Risk of Cardiovascular Disease in Diabetes Mellitus Type 2

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