Study on interaction between gold nanorod and bovine serum albumin

Pan, Bifeng; Cui, Daxiang; Xu, Ping; Li, Qing; Huang, Tuo; He, Rong; Gao, Feng

doi:10.1016/j.colsurfa.2006.09.002

Cited by 95 publications

(55 citation statements)

References 25 publications

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“…According to this method the BSA fluorescence intensity (F) scales up with increase in streptomycin concentration according to the following relation [36]:…”

Section: Fluorescence Measurementsmentioning

confidence: 99%

Binding of streptomycin with bovine serum albumin: Energetics and conformational aspects

Jha

Kishore

2009

Thermochimica Acta

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“…According to this method the BSA fluorescence intensity (F) scales up with increase in streptomycin concentration according to the following relation [36]:…”

Section: Fluorescence Measurementsmentioning

confidence: 99%

Binding of streptomycin with bovine serum albumin: Energetics and conformational aspects

Jha

Kishore

2009

Thermochimica Acta

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“…12,28 Even with all that is currently known about native BSA, detailed information on BSA-AuNP conjugates is relatively sparse. 6,7,9,15,[38][39][40][41][42][43] Additionally, a few related studies have examined the adsorption and conformation of BSA onto Au flats.…”

Section: Introductionmentioning

confidence: 99%

Adsorption and Conformation of Serum Albumin Protein on Gold Nanoparticles Investigated Using Dimensional Measurements and in Situ Spectroscopic Methods

et al. 2011

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ABSTRACT:The adsorption and conformation of bovine serum albumin (BSA) on gold nanoparticles (AuNPs) were interrogated both qualitatively and quantitatively via complementary physicochemical characterization methods. Dynamic light scattering (DLS), asymmetric-flow field flow fractionation (AFFF), fluorescence spectrometry, and attenuated total reflectanceFourier transform infrared (ATR-FTIR) spectroscopy were combined to characterize BSA-AuNP conjugates under fluid conditions, while conjugates in the aerosol state were characterized by electrospray-differential mobility analysis (ES-DMA). The presence of unbound BSA molecules interferes with DLS analysis of the conjugates, particularly as the AuNP size decreases (i.e., below 30 nm in diameter). Under conditions where the γ value is high, where γ is defined as the ratio of scattering intensity by AuNPs to the scattering intensity by unbound BSA, DLS size results are consistent with results obtained after fractionation by AFFF. Additionally, the AuNP hydrodynamic size exhibits a greater proportional increase due to BSA conjugation at pH values below 2.5 compared with less acidic pH values (3.4-7.3), corresponding with the reversibly denatured (E or F form) conformation of BSA below pH 2.5. Over the pH range from 3.4 to 7.3, the hydrodynamic size of the conjugate is nearly constant, suggesting conformational stability over this range. Because of the difference in the measurement environment, a larger increase of AuNP size is observed following BSA conjugation when measured in the wet state (i.e., by DLS and AFFF) compared to the dry state (by ES-DMA). Molecular surface density for BSA is estimated based on ES-DMA and fluorescence measurements. Results from the two techniques are consistent and similar, but slightly higher for ES-DMA, with an average adsorbate density of 0.015 nm -2 . Moreover, from the change of particle size, we determine the extent of adsorption for BSA on AuNPs using DLS and ES-DMA at 21°C, which show that increasing the concentration of BSA increases the measured change in AuNP size. Using ES-DMA, we observe that the BSA surface density reaches 90% of saturation at a solution phase concentration between 10 and 30 μmol/L, which is roughly consistent with fluorescence and ATR-FTIR results. The equilibrium binding constant for BSA on AuNPs is calculated by applying the Langmuir equation, with resulting values ranging from 0.51 Â 10 6 to 1.65 Â 10 6 L/mol, suggesting a strong affinity due to bonding between the single free exterior thiol on N-form BSA (associated with a cysteine residue) and the AuNP surface. Moreover, the adsorption interaction induces a conformational change in BSA secondary structure, resulting in less R-helix content and more open structures (β-sheet, random, or expanded).

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“…Among the different biosensors, biosensors based on nanomaterials are very useful for biomolecular recombination pathogenic diagnosis and environmental monitoring applications [20][21][22][23]. The nano-size of a surface and the fast interaction with the target analytes on the surface make biosensors based on a nanomaterial able to detect analytes even in small volumes.…”

Section: Introductionmentioning

confidence: 99%

“…To have a highly sensitive biosensor with a fast response and a specific application, the choice of the material of sensor is very important. Several nanomaterials such as nanowires/nanotubes [24], gold nanoparticles [23], magnetic nanoparticles [25], carbon nanotubes [26] and quantum dots [27] have been investigated to evaluate their application as biosensors. Experiments have looked at their physical, chemical, mechanical, magnetic and optical properties for increased sensitivity, faster response and specific biomolecules recognition.…”

Section: Introductionmentioning

confidence: 99%

Synthesising Metal Oxide Materials and Their Composite Nanostructures for Sensing and Optoelectronic Device Applications

Khun¹

2015

Linköping Studies in Science and Technology. Dissertations

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Research on nanomaterials has been revolutionized in the last few years because of the attractive properties they have in comparison to the bulk phase of similar materials. These properties are physical, chemical, catalytic and optical. Among these nanomaterials, the metal oxide nanostructures have become of particular interest to scientists for the development of different optical, biochemical and biomedical nanodevices. In the present research work using the advantageous features of nanotechnology, high performance nanodevices for optoelectronics with a wide band gap compound nanostructure and highly sensitive sensor devices have been demonstrated. The nanotechnology is used to fabricate sensitive and precise nanodevices based on nanomaterials for the application of sensing.Among metal oxide nanostructures, ZnO, CuO and NiO are attractive materials because of their unique properties; their high surface area to volume ratio, their energy band gap of 3.37 eV, 1.2 eV and 3.7 eV, respectively, biocompatibility, high electron mobility, fast electron transfer rate and they are environmental-friendly in many applications. When used in sensor devices, nanomaterials have indicated high selectivity for possible use to detect the various analytes even in small volumes. Metal oxide nanostructures have shown to be good for optoelectronic nanodevices because of their electrical characteristics, high optical absorption and low-processing temperature.In this thesis, the synthesis of different morphologies of metal oxide semiconductor nanostructures and their composite using the hydrothermal method are demonstrated for various applications. This thesis is divided into three parts:In the first part of this research work, the fabrication of well-aligned ZnO nanorods using different concentrations of composite seed layer of inorganic and organic materials when using the hydrothermal growth method is presented. The effect of the composite seed layer on the i Abstract alignment, density and optical properties of the grown ZnO nanorods is investigated (paper I).Utilizing the advantage of ZnO nanostructure, a comparative study of ZnO nanorods and thin films for chemical and biosensing application was carried out. The ZnO nanorods and thin films were functionalized with strontium ionophore membrane, immobilized the galactose oxidase and lactate oxidase for determining the strontium ions, D-galactose and L-lactic acid, respectively (paper II).In the second part, the effects of different urea concentrations on the morphology of CuO nanostructures is studied as described in paper III. Moreover, CuO nanoflowers were functionalized with cadmium ion ionophore for the detection of Cd ions, while CuO nanosheets were grown by the low temperature growth method and were used for the development of a nonenzymatic glucose sensor, respectively (Paper IV).In the last part of this thesis, composite nanostructures of CuO/ZnO and NiO/ZnO were applied to develop dopamine sensor and fast sensitive UV photodetector, respectively. A nanohybrid of C...

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Study on interaction between gold nanorod and bovine serum albumin

Cited by 95 publications

References 25 publications

Binding of streptomycin with bovine serum albumin: Energetics and conformational aspects

Binding of streptomycin with bovine serum albumin: Energetics and conformational aspects

Adsorption and Conformation of Serum Albumin Protein on Gold Nanoparticles Investigated Using Dimensional Measurements and in Situ Spectroscopic Methods

Synthesising Metal Oxide Materials and Their Composite Nanostructures for Sensing and Optoelectronic Device Applications

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