Study on interactions of aminoglycoside antibiotics with calf thymus DNA and determination of calf thymus DNA via the resonance Rayleigh scattering technique

Qiao, Man; Li, Chunyan; Shi, Ying; Liu, Shaopu; Liu, Zhongfang; Hu, Xiaoli

doi:10.1002/bio.2876

Cited by 11 publications

(5 citation statements)

References 46 publications

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“…According to the reports in some literatures, the modes in which foreign compounds interact with DNA are summarized as three types: intercalation binding between molecular and stacked base pairs in DNA, groove binding between molecular and major or minor grooves in DNA double-helix, and electrostatic binding between charged molecule and the external DNA backbone (60, 61). UV-visible spectral is a useful tool to study the interaction between E. coli DNA and foreign antibacterial agents (62). As we known, the hypochromic effect combined with a red shift in wavelength is observed only when the axial change of DNA conformation occurs, causing by the foreign molecular intercalating into DNA (63).…”

Section: Discussionmentioning

confidence: 99%

The key lethal effect existed in the antibacterial behavior of short, medium, and long chain fatty acid monoglycerides onEscherichia coli

Song

Xiong

Lou

et al. 2018

Preprint

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Monoglyceride is an amphiphilic molecule with promising antimicrobial 22 activity for bacteria; however, the key lethal effect in its antibacterial behavior was still 23 unknown. In the study, monobutyrate (MB), monolaurate (ML), monomyristate (MM) 24 were selected to represent the short, medium, and long chain monoglycerides to compare 25 their inhibitory effect against Escherichia coli, and a new dose-dependent inhibitory 26 mechanism was proposed by the key lethal effect. The minimal inhibitory concentration 27 and antibacterial curve showed a huge diversity existed in biology activity of MB, ML 28 and MM. The results in scanning electron microscopy and flow cytometry assay 29 indicated that the interference level of MB on cell membrane was obviously weaker than 30 that of ML and MM, while the latter two had similar performance in increasing cell 31 permeability at low doses. The results presented in UV-Vis spectroscopy, cell cycle and 32 biomacromolecules synthesis inhibition assay showed that the cell cycle of Escherichia 33 coli was obviously affected by three monoglycerides at doses near MIC, which was 34 therefore regarded as the key lethal effect. The reason for the better biological activity of 35 MM than ML was the stronger interference ability on bacterial cell cycle. In addition, an 36 expanded antibacterial mode was raised that cell permeability increase at low doses was 37 antimicrobial basis, cell cycle arrest at medium doses played the key lethal effect, and 38 cell lysis at high doses was the result of combined action. 39 40

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Section: Discussionmentioning

confidence: 99%

The key lethal effect existed in the antibacterial behavior of short, medium, and long chain fatty acid monoglycerides onEscherichia coli

Song

Xiong

Lou

et al. 2018

Preprint

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“…Spectroanalytical techniques are powerful tools in determining the method of DNA interaction and binding strength of many compounds. These include UV–visible absorption titration, isothermal titration calorimetry, fluorescence spectroscopy, circular dichroism spectroscopy, molecular docking studies, cyclic voltammetry, differential pulse voltammetry, hydrodynamic measurement, atomic force spectroscopy, resonance Rayleigh scattering technique and nuclear magnetic resonance …”

Section: Introductionmentioning

confidence: 99%

Fluorescence and absorption studies of DNA–Pd(II) complex interaction: Synthesis, spectroanalytical investigations and biological activities

et al. 2019

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Novel palladium(II) complexes (7a–7e) of substituted quinoline derivatives were synthesized. The complexes were characterized using various techniques such as thermogravimetric analysis (TGA), elemental analysis, conductance measurement, mass, absorption, infra‐red (IR), 1H NMR, 13C NMR and energy‐dispersive X‐ray spectroscopy (EDX). Complexes for herring sperm DNA (HS DNA) binding were explored and absorption titration and the binding constant (Kb) as well as Gibb's free energy were evaluated. Complex 7d exhibited the highest binding constant, therefore the thermodynamic parameters of 7d at different temperatures were evaluated. To support the results of the absorption titration, fluorescence titration, viscosity measurement and molecular docking studies were performed. The fluorescence quenching data as evaluated from Stern–Volmer equation were used to calculate KSV, Kf and the number of binding sites. The results of all these studies were in good agreement with the absorption study. DNA electrophoretic mobility was performed to explore the possible application of metal complexes as artificial metallonucleases. The antibacterial activity of the complexes was accessed against different pathogenic bacteria and cytotoxicity was measured using brine shrimp and S. pombe.

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“…Starting with the 2000s, a series of studies underlined the utility of the method in the assay of pharmaceuticals as ion pairs with organic dyes [79][80][81] or using a counterion attached to nanoparticles [82,83] but also for unravelling of their interaction mechanisms with macromolecules of biological interest (transport proteins, DNA) [84][85][86]. Recent studies have highlighted the potential of this technique to elucidate the action mechanisms of pharmaceutical substances at the molecular level: the mechanism of interaction of oridonin (natural substance with anticancer effect) with DNA macromolecule was revealed [87]; also, the molecular mechanism by which quercetol affects the bioavailability of propranolol was explained [88].…”

Section: Light Scattering Spectrometrymentioning

confidence: 99%

Ion-Pair Spectrophotometry in Pharmaceutical and Biomedical Analysis: Challenges and Perspectives

Florea¹,

Ilie²

2017

Spectroscopic Analyses - Developments and Applications

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Experimental and theoretical studies of the mechanisms that underlay ion-pair formation, their properties and applications in various fields have been and still are focused by researchers since the introduction of the concept in 1926, by Bjerrum. Ion pairs are distinct chemical entities, electrically neutral, formed between ions of opposite charge and held together by Coulomb forces, without formation of a covalent bond. Investigation methods used are various, from classical conductometric measurements to up-to-date methods, such as spectrophotometry, chromatography and capillary electrophoresis. In the pharmaceutical field, ion pairs were used to develop methods of separation, identification and assay for the active substances in complex matrices, to obtain pharmaceutical formulations with controlled release and to explain the mechanisms of transport and action for certain drugs. The chapter is an attempt to describe new trends in the spectrophotometry of ion pairs and their applications in the pharmaceutical field. The development of the concept and types of ion pairs are first presented; further, examples of applications using molecular absorption, fluorimetry and resonance light scattering spectrophotometry are presented. Based on the literature data and the authors' experience in the field, challenges and perspectives in the ion-pair spectrophotometry are also considered.

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Study on interactions of aminoglycoside antibiotics with calf thymus DNA and determination of calf thymus DNA via the resonance Rayleigh scattering technique

Cited by 11 publications

References 46 publications

The key lethal effect existed in the antibacterial behavior of short, medium, and long chain fatty acid monoglycerides onEscherichia coli

The key lethal effect existed in the antibacterial behavior of short, medium, and long chain fatty acid monoglycerides onEscherichia coli

Fluorescence and absorption studies of DNA–Pd(II) complex interaction: Synthesis, spectroanalytical investigations and biological activities

Ion-Pair Spectrophotometry in Pharmaceutical and Biomedical Analysis: Challenges and Perspectives

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