Study on Isolation and Identification of <i>Salmonella</i> and <i>Escherichia coli</i> from Different Poultry Feeds of Savar Region of Dhaka, Bangladesh

Abstract

The study was conducted aiming at the isolation and identification of Salmonella and Escherichia coli (E. coli) from different brands of poultry feeds sold in Savar, Dhaka, Bangladesh. Seven different poultry feeds were subjected to microbiological analysis. All these samples were analyzed by culturing in different media such as nutrient broth (NB), nutrient Agar (NA), SS Agar (Salmonella-Shigella Agar), BGA (brilliant-green Agar), Mac Conkey, DHL and EMB (eosin methylene blue) media. Total bacterial colonies of all the samples were counted separately on the nutrient Agar media. Hence, bacteria were counted as 9.5×105 in the feed sample C (Layer) which was found to be the highest in number among the poultry feeds. Total viable count (TVC) of Salmonella and E. coli in the feed samples were as 0 to 6.75×104 and 0 to 3.05×104 respectively. Both organisms were found in 71.43% and 57.14% of the analyzed feed samples, respectively. The highest number of Salmonella was found in sample C (Layer) feeds and that of E. coli was found in sample B (Grower) feeds. The widespread occurrence of Salmonella and E. coli in poultry feeds reinforces the need for effective control measures, hygiene in processing and handling of feeds. Keywords: Salmonella; Escherichia coli; Poultry feeds; Total viable count; Contamination; Hygiene. © 2011 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. doi:10.3329/jsr.v3i2.7128 J. Sci. Res. 3 (2), 403-411 (2011)

Microbiological quality of commercially available poultry feeds sold in Bangladesh

“…Possible reason for the absence of fungal count in feed sample A, B and C might be inclusion of antifungal agents, use of high temperature during preparation and manufacturing. The study showed that the percentage occurrence of E. coli was 65% (Table 5) which is in line with the findings of Akond et al (2009), Chowdhuri et al (2011) andDa Costa et al (2007) where it was 58%, 57.14% and 50% respectively. On the other hand the percentage of Salmonella spp.…”

“…The feed samples were processed as described by Chowdhuri et al (2011) by homogenizing 10 gms of each sample in 90 ml of peptone water in the beaker and mixed thoroughly. Then 900 μl PBS was taken to each of the small bottles accordingly.…”

“…Poultry feeds can be contaminated directly and indirectly through contact with soil, rodent, birds, dust, human carrier, sewage or water during processing and storage (Ezekiel et al, 2011). Poultry feeds can potentially become contaminated with food borne pathogenic microorganisms during harvesting, processing, handling and marketing of the bagged feeds (Chowdhuri et al, 2011). Poultry feeds contaminated with bacteria pathogenic to humans can contribute to human food borne illness through the feed-poultry-food-human chain.…”

Nanoencapsulation of Nisin and Ethylenediaminetetraacetic Acid in Niosomes and Their Antibacterial Activity

“…Contamination of food pathogens in animal feeds and foods has been a major concern in the distribution and storage of the products worldwide [1,2]. Among various preservations, biopreservation using natural derived compounds is more preferable compared to synthetic chemicals [3].…”

Role of egg in food born salmonellosis: A public health issue.

“…Contamination mainly by moist environment of market and poultry house, poultry feeds, unhygienic practice of farm handlers and surrounding environment 4. Another study was done to detect the Salmonella contamination rate from poultry feeds, where 71.43% Salmonella was detected from poultry feeds from different market in Savar area 38 .…”