Study on &lt;i&gt;Angelica sinensis&lt;/i&gt; Endophytic Fungi and its Antibacterial Activity

Yang, Ming; Wang, Yong Gang; Liu, Xiaofeng; Wu, Jing; Qian, Jiao

doi:10.4028/www.scientific.net/amr.641-642.816

Cited by 4 publications

(5 citation statements)

References 6 publications

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“…The results obtained in this study also agree with those published previously in the literature (Moein et al, 2008). In addition, there were few studies on the activity of A. sinensis endophytes besides (Yang et al, 2013). The antimicrobial activity of isolated endophytes from A. sinensis were detected using the paper disc diffusion method with five tested strains, including E. coli, S. aureus, B. licheniformis, P. aeruginosa, and Str.…”

Section: Discussionsupporting

confidence: 91%

“…and Aspergillus sp. The results also agreed with other previous reports that members of a variety of common fungal genera, such as Alternaria sp., Aspergillus sp., Penicillium sp., and Fusarium sp., are usually isolated from a variety of plants around the world (Yang et al, 2013;Wȩżowicz et al, 2014). In addition, organ specificity of endophytes were observed (Kusari et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…In addition, there were few studies on the activity of A . sinensis endophytes besides ( Yang et al, 2013 ). The antimicrobial activity of isolated endophytes from A .…”

Section: Discussionmentioning

confidence: 99%

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Diversity and Bioactivity of Endophytes From Angelica sinensis in China

et al. 2020

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Plant seeds are not merely reproductive organs, they are also carriers of microorganism, particularly, inherent and non-invasive characteristic endophytes in host plant. Therefore, in this study, the endophytic diversity of Angelica seeds was studied and compared with endophytes isolated from healthy leaves, stems, roots, and seeds of A. sinensis using 20 different media. The metabolites of endophytic strains were evaluated with six different methods for their antioxidant activity and the paper disc diffusion method for antimicrobial activities. As a result, 226 endophytes were isolated. Compared with the biodiversity and abundance of uncultured fungi from Angelica seed, the result showed that the most frequent endophytic fungi were Alternaria sp. as seen in artificial media; moreover, compared with artificial media, the pathogenic fungi, including Fusarium sp. and Pseudallescheria sp., were not found from the Angelica seed, the results suggested it may not be inherent endophytes in plants. In addition, bacteria from seven phyla were identified by high-throughput sequencing, while five phyla of endophytic bacteria were not isolated on artificial media including Proteobacteria, Actinobacteria, Bacteroidetes, Microgenomates, and Saccharibacteria. Furthermore, the sample JH-4 mycelium displayed the best antioxidant activity, and the active constituent may be a flavonoid as determined by total phenol and flavonoid content. Moreover, YH-12-1 mycelium had strong inhibitory activity against the five tested strains and the minimum inhibitory concentration (MIC) against Pseudomonas aeruginosa and Streptococcus pneumoniae was found to be 25 µg/mL. Our results confirm that plant endophytes are rich in biodiversity and contain important resource of many uncultured microorganisms.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

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Diversity and Bioactivity of Endophytes From Angelica sinensis in China

et al. 2020

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“…Its root is a widely used medicine-food Chinese medicinal herb known as Danggui (Angelica sinensis, AS) [1,2]. AS is rich in polysaccharides, essential oils, flavonoids, and organic acids [3,4]. AS is often used to treat blood deficiency, menstrual disorders, and constipation due to its properties as a blood tonic, menstrual pain reliever, and laxative [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Automated Grading of Angelica sinensis Using Computer Vision and Machine Learning Techniques

Zhang,

Xiao,

Wang

et al. 2024

Agriculture

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Angelica sinensis (Oliv.) Diels, a member of the Umbelliferae family, is commonly known as Danggui (Angelica sinensis, AS). AS has the functions of blood tonic, menstrual pain relief, and laxatives. Accurate classification of AS grades is crucial for efficient market management and consumer health. The commonly used method to classify AS grades depends on the evaluator’s observation and experience. However, this method has issues such as unquantifiable parameters and inconsistent identification results among different evaluators, resulting in a relatively chaotic classification of AS in the market. To address these issues, this study introduced a computer vision-based approach to intelligently grade AS. Images of AS at five grades were acquired, denoised, and segmented, followed by extraction of shape, color, and texture features. Thirteen feature parameters were selected based on difference and correlation analysis, including tail area, whole body area, head diameter, G average, B average, R variances, G variances, B variances, R skewness, G skewness, B skewness, S average, and V average, which exhibited significant differences and correlated with grades. These parameters were then used to train and test both the traditional back propagation neural network (BPNN) and the BPNN model improved with a growing optimizer (GOBPNN). Results showed that the GOBPNN model achieved significantly higher average testing precision, recall, F-score, and accuracy (97.1%, 95.9%, 96.5%, and 95.0%, respectively) compared to the BPNN model. The method combining machine vision technology with GOBPNN enabled efficient, objective, rapid, non-destructive, and cost effective AS grading.

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“…However, endophytic fungal strains which belonged to Alternaria sp (AS-R109) and Penicillium sp (AS-R203) were isolated from A. sinensis. Antimicrobial activity of the metabolites produced by the species was detected via a filter paper method using S. aureus, E. coli, Pseudomonas aeruginosa, and Klebsiella pneumonia (Yang et al 2013). Little is known about the interaction between A. sinensis and endophytes, particularly the effects of endophytes on the growth of A. sinensis and synthesis of secondary metabolites in A. sinensis.…”

Section: Introductionmentioning

confidence: 99%

Study on effects of endophytes on growth and production of Z-Ligustilide and ferulic acid in Angelica sinensis

Zhang

Wang

et al. 2016

Braz. J. Bot

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With the application of artificial fertilizers and pesticides, the quality of the artificial medicinal plants has been found to be in a marked decline. The present paper focuses on the separation of endophytes from wild Angelica sinensis and the effects of endophytes on the growth of A. sinensis and synthesis of secondary metabolites. In this study, the endophytes, namely actinomycetes, fungi, and bacteria, were isolated. Z-ligustilide and ferulic acid were analyzed by HPLC. Our results confirmed that the contents of Z-ligustilide and ferulic acid showed an obvious increase on adding the endophytic fungi, but the additions of the endophytic actinomycetes and bacteria had shown inhibiting effects. Compared with the control group, the contents of Z-ligustilide and ferulic acid in the fungi group increased by 4.12-and 6.53fold, respectively. This work demonstrates the fact that endophytic fungi can enhance the growth of plants and the production of natural products in A. sinensis.

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Study on <i>Angelica sinensis</i> Endophytic Fungi and its Antibacterial Activity

Cited by 4 publications

References 6 publications

Diversity and Bioactivity of Endophytes From Angelica sinensis in China

Diversity and Bioactivity of Endophytes From Angelica sinensis in China

Automated Grading of Angelica sinensis Using Computer Vision and Machine Learning Techniques

Study on effects of endophytes on growth and production of Z-Ligustilide and ferulic acid in Angelica sinensis

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