Study on mechanism of elemene reversing tumor multidrug resistance based on luminescence pharmacokinetics in tumor cells <i>in vitro</i> and <i>in vivo</i>

Chen, Liying; Chen, Zhi; Zheng, Shaokuan; Fan, Liping; Zhu, Lixin; Yu, Jianan; Tang, Chaoyuan; Liu, Qi; Xiong, Yang

doi:10.1039/d0ra00184h

Cited by 3 publications

(3 citation statements)

References 38 publications

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“…These two approaches have resulted in luciferin analogues with improved characteristics for in vivo imaging, as discussed below. [15], [16], [18], [19], [20], [21], [24], [28], [29], [30], [31], [32], [33], [34], [35], [36], [ Miller et al developed CycLuc1, in which a pyrrolidine moiety is fused to the benzothiazole moiety of DLH2. The wavelength of CycLuc1 is 599 nm (Km = 0.10 and 1.06 μM in Table 2), which is longer than that of DLH2 due to electron donation from the fused N-hetero ring [75].…”

Section: Luciferin Analogues That Produce Nir Lightmentioning

confidence: 99%

“…Luciferin–luciferase reactions are also used for in vivo real-time monitoring of gene expression, cell numbers, and other biological events. Specifically, firefly BLI has been utilized for in vivo study in numerous mouse models [ 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 ]. For instance, it has been used to quantify tumor cells [ 37 ] and monitor gene expression in transgenic mouse models [ 38 ].…”

Section: Introductionmentioning

confidence: 99%

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How to Select Firefly Luciferin Analogues for In Vivo Imaging

Saito-Moriya

Nakayama²,

Kamiya

et al. 2021

IJMS

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Bioluminescence reactions are widely applied in optical in vivo imaging in the life science and medical fields. Such reactions produce light upon the oxidation of a luciferin (substrate) catalyzed by a luciferase (enzyme), and this bioluminescence enables the quantification of tumor cells and gene expression in animal models. Many researchers have developed single-color or multicolor bioluminescence systems based on artificial luciferin analogues and/or luciferase mutants, for application in vivo bioluminescence imaging (BLI). In the current review, we focus on the characteristics of firefly BLI technology and discuss the development of luciferin analogues for high-resolution in vivo BLI. In addition, we discuss the novel luciferin analogues TokeOni and seMpai, which show potential as high-sensitivity in vivo BLI reagents.

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Section: Luciferin Analogues That Produce Nir Lightmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

How to Select Firefly Luciferin Analogues for In Vivo Imaging

Saito-Moriya

Nakayama²,

Kamiya

et al. 2021

IJMS

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“…In recent biological research, BLI technology has observed biological events in vivo [2][3][4][5][6][7][8]. For example, in cancer research, BLI has been applied to real-time monitoring of gene expression, cell numbers, and other biological events in transgenic mouse models [9][10][11][12][13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Near-Infrared Luciferin Analogs for In Vivo Optical Imaging

Saito-Moriya¹,

Obata²,

Maki³

2021

Bioluminescence - Technology and Biology

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The firefly bioluminescence reaction has been exploited for in vivo optical imaging in life sciences. To develop highly sensitive bioluminescence imaging technology, many researchers have synthesized luciferin analogs and luciferase mutants. This chapter first discusses synthetic luciferin analogs and their structure–activity relationships at the luminescence wavelength of the firefly bioluminescence reaction. We then discuss the development of luciferin analogs that produce near-infrared (NIR) light. Since NIR light is highly permeable for biological tissues, NIR luciferin analogs might sensitively detect signals from deep biological tissues such as the brain and lungs. Finally, we introduce two NIR luciferin analogs (TokeOni and seMpai) and a newly developed bioluminescence imaging system (AkaBLI). TokeOni can detect single-cell signals in mouse tissue and luminescence signals from marmoset brain, whereas seMpai can detect breast cancer micro-metastasis. Both reagents are valid for in vivo bioluminescence imaging with high sensitivity.

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Liposomal Codelivery of Doxorubicin and Curcumin Sensitizes Antitumor Activity and Reduces Tumor Metastasis

Kim,

Park,

Hong

et al. 2024

ACS Appl. Bio Mater.

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Multidrug resistance (MDR) is a major obstacle to traditional cancer treatment using chemotherapeutic agents like doxorubicin (DOX). MDR affects drug dosage regimens and enables the recurrence and metastasis of cancer. Because DOX causes severe side effects at high dosages, it is important to use an MDR modulator to make cancer cells sensitive to DOX. This work focused on a liposome-based codelivery system containing curcumin (CUR) and DOX, focusing on CUR as an MDR modulator. The synergistic effect was maximized when the ratio of DOX and CUR was 1:1, and the synthesis of liposomal drugs was successfully verified. In addition, a successful MDR reversal effect was demonstrated through rhodamine 123 assay, Western blotting, and immunofluorescence. Compared to the conventional DOX treatment, the dual-drug treatment exhibits a significantly improved anticancer effect. In the murine metastasis 4T1 IP tumor model, the dual-drug-encapsulating liposomes successfully suppressed tumor growth and reversed the tumoral effect (omental tumor metastasis, fat, and muscle weight loss) into a normal state.

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Study on mechanism of elemene reversing tumor multidrug resistance based on luminescence pharmacokinetics in tumor cells in vitro and in vivo

Abstract: The correlation between efflux functionality of ATP-binding cassette transporters and tumor multidrug resistance reversing via elemene was investigated using bioluminescence imaging (BLI) technology and luciferase reporter gene technology.

Cited by 3 publications

References 38 publications

How to Select Firefly Luciferin Analogues for In Vivo Imaging

How to Select Firefly Luciferin Analogues for In Vivo Imaging

Near-Infrared Luciferin Analogs for In Vivo Optical Imaging

Liposomal Codelivery of Doxorubicin and Curcumin Sensitizes Antitumor Activity and Reduces Tumor Metastasis

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