Study on molecularly imprinted nanoparticle modified microplates for pseudo-ELISA assays

Cenci, Lucia; Piotto, Chiara; Bettotti, Paolo; Bossi, Alessandra Maria

doi:10.1016/j.talanta.2017.10.018

Cited by 29 publications

(17 citation statements)

References 48 publications

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“…MIPs have gained popularity ever since Mosbach’s group, in 1993, reported their application in a pseudo-immunoassay for the determination of drugs 4 , whereby for the first time, MIPs were coined ‘antibody mimics’ 5 . Hence, MIPs have been around for some time now and they have been widely developed and applied in solid-phase extraction 6,7 , sensors 8,9 , pseudo-immunoassays 10,11 , drug delivery 12,13 and very recently for optical bioimaging 14–16 . Nevertheless, despite many efforts to make MIPs become ‘ a useful general alternative to antibodies ’ 5 , they are still not very much commercialized.…”

Section: Introductionmentioning

confidence: 99%

Solid-phase synthesis of molecularly imprinted polymer nanolabels: Affinity tools for cellular bioimaging of glycans

Rangel

Laclef

et al. 2019

Sci Rep

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Hyaluronic acid (HA) is a glycosaminoglycan that plays many roles in health and disease and is a key biomarker of certain cancers. Therefore, its detection at an early stage, by histochemical methods, is of importance. However, intracellular HA can be masked by other HA-binding macromolecules, rendering its visualization somehow problematic. We show that fluorescent molecularly imprinted polymer nanogels (MIP-NPs), can localize and detect intracellular HA. MIP-NPs were synthesized by solid-phase synthesis on glass beads (GBs). GBs were functionalized with terminal alkyne groups on which an azide derivative of the template molecule glucuronic acid was immobilized via click chemistry. Immobilization via the anomeric carbon left the template’s carboxyl moiety free to enable strong stoichiometric electrostatic interactions with a benzamidine-based functional monomer, to confer selective recognition to the MIP-NPs. Due to the two-point orientation of the template, the resulting MIP-NPs were endowed with improved binding site homogeneity and specificity, reminiscent of monoclonal antibodies. These synthetic antibodies were then applied for probing and staining HA, of which glucuronic acid is a substructure (epitope), on human epidermal cells. Their excellent sensitivity, small size and water compatibility, enabled the MIP-NPs to visualize HA, as evidenced by confocal fluorescence micrographs.

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Section: Introductionmentioning

confidence: 99%

Solid-phase synthesis of molecularly imprinted polymer nanolabels: Affinity tools for cellular bioimaging of glycans

Rangel

Laclef

et al. 2019

Sci Rep

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“…[67,68] On the other hand, the lack of reproducibility and the difficult coating of the microplates wells makes it unfeasible to be used in quantitative analyses. [46,69] In general, a reasonable number of studies employing MIPs in off-line techniques can be found (Table 1), probably because less sophisticated equipment is required. However, sample handling can amplify the experimental errors and decrease analytical frequency.…”

Section: Off-line Solid-phase Extractionmentioning

confidence: 99%

“…Cenci et al [46] took a closer look at the preparation and stability of the pseudo-ELISA plates. Fluorescent MIPs of acrylamide, methacrylic acid, and acryloxyethyl thiocarbamoyl Rhodamine B were prepared by precipitation polymerization.…”

Section: Pseudo-elisamentioning

confidence: 99%

Molecularly Imprinted Solid Phase Extraction Aiding the Analysis of Disease Biomarkers

Silva

Tavares

Faria

et al. 2020

Critical Reviews in Analytical Chemistry

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Low concentrations of biomarkers as well as the complexity of biological samples make the clinical diagnoses of several diseases a challenging task. Sample preparation protocols remain a fundamental piece in the puzzle of analytical processes, and smart sorbents including molecularly imprinted polymers (MIPs) have been successfully used in this case. In this review, we depict the state of art for the rational design of MIPs to be used in solid phase extraction of disease biomarkers from biological samples. The topics are divided into (1) strategies for MIP syntheses, (2) setups for sample preparation protocols with MIPs, (3) the applications of these combined principles in the analyses of different classes of disease biomarkers, and (4) remaining challenges and future trends for the application of Molecular Imprinting Technology in sample preparation for clinical diagnosis.

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“…One good thing about MIP is that once the synthesis method is established, it can be further utilized as a versatile binding site for selectively recognizing the target molecule in multiple forms. Interestingly, the MIP has been applied to the detection of many other iron status markers, such as quantification of hepcidin by SPR and pseudo‐ELISA methods (Cenci et al, 2015; Cenci, Piotto, Bettotti, & Maria Bossi, 2018), separation and detection of Tf based on the so‐called magnetic fluorescence molecularly imprinted nanoparticles (MFMINPs) (Y. D. Zhang, Huang, Ma, Liu, & Zhang, 2018), and ferritin sensing achieved by arrays of carbon‐nanotube tips coated with nonconductive MIP and readout via electrochemical impedance spectroscopy (Cai et al, 2010). For better depicting the iron status in point‐of‐care testing, a dual‐functional microfluidic paper analytical device (μPAD) has been developed for Fe 3+ and ferritin analysis (Hu et al, 2017).…”

Section: Nanotechnology In Coordination With Iron Homeostasismentioning

confidence: 99%

Nano‐sensing and nano‐therapy targeting central players in iron homeostasis

2020

WIREs Nanomed Nanobiotechnol

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Iron plays vital roles in many life activities and it is strictly controlled via elaborate metabolic system. Growing evidence has suggested that the dysfunctional iron homeostasis is implicated to many refractory diseases including cancers and neurodegenerations. Systemic and cellular iron are regulated through different pathways but are meanwhile interconnecting with each other via a few key regulators, whose abnormal expressions are often found to be the root causes of many iron disorders. Nano‐sensing techniques have enabled the detection and monitoring of such central players, which provide rich information for the iron homeostasis profile through multiplexing and flexible designs. In addition to general sensing, nanoprobes are capable of target imaging and precise local access, which are particularly beneficial for revealing the conditions of intra‐/extracellular environments. Nanomaterials have also been applied in some therapies, targeting the aberrant iron metabolism. Various iron uptake pathways have been utilized for target drug delivery and iron level manipulation, while abnormal iron content is notably useful in tumor killing. With brief introduction to the significance of iron homeostasis, this review includes recent works regarding the nanotechnology that has been applied in iron‐related diagnostic and therapeutic applications. This article is categorized under: Diagnostic Tools > Biosensing Therapeutic Approaches and Drug Discovery > Nanomedicine for Oncologic Disease Diagnostic Tools > in vivo Nanodiagnostics and Imaging

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Study on molecularly imprinted nanoparticle modified microplates for pseudo-ELISA assays

Cited by 29 publications

References 48 publications

Solid-phase synthesis of molecularly imprinted polymer nanolabels: Affinity tools for cellular bioimaging of glycans

Solid-phase synthesis of molecularly imprinted polymer nanolabels: Affinity tools for cellular bioimaging of glycans

Molecularly Imprinted Solid Phase Extraction Aiding the Analysis of Disease Biomarkers

Nano‐sensing and nano‐therapy targeting central players in iron homeostasis

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