Study on Multi-Elements in Traditional Chinese Medicines &lt;i&gt;Ophiopogon japonicus&lt;/i&gt; and Lotus Seeds by the ICP-AES with Microwave Digestion

Zhang, Sheng Bang; Ji, Xiao Wu; Liu, Cui Ping

doi:10.4028/www.scientific.net/amr.535-537.1126

Cited by 4 publications

(3 citation statements)

References 27 publications

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“…The sample (0.3 g of dry black or brown seeds for each sample) was prepared by microwave digestion in HNO 3 -H 2 O 2 , and ultrapure water was added to obtain a final volume of 25 mL [ 74 ]. Then, these were analyzed using an inductively coupled plasma (ICP-OES) spectrometer (Optima 7300 DV, Perkin-Elmer, USA).…”

Section: Methodsmentioning

confidence: 99%

Transcriptomic profiling of genes in matured dimorphic seeds of euhalophyte Suaeda salsa

Zhao

Duan

et al. 2017

BMC Genomics

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Background Suaeda salsa (S. salsa) is a euhalophyte with high economic value. S. salsa can produce dimorphic seeds. Brown seeds are more salt tolerant, can germinate quickly and maintain the fitness of the species under high saline conditions. Black seeds are less salt tolerant, may become part of the seed bank and germinate when soil salinity is reduced. Previous reports have mainly focused on the ecophysiological traits of seed germination and production under saline conditions in this species. However, there is no information available on the molecular characteristics of S. salsa dimorphic seeds.ResultsIn the present study, a total of 5825 differentially expressed genes were obtained; and 4648 differentially expressed genes were annotated based on a sequence similarity search, utilizing five public databases by transcriptome analysis. The different expression of these genes may be associated with embryo development, fatty acid, osmotic regulation substances and plant hormones in brown and black seeds. Compared to black seeds, most genes may relate to embryo development, and various genes that encode fatty acid desaturase and are involved in osmotic regulation substance synthesis or transport are upregulated in brown seeds. A large number of differentially expressed genes related to plant hormones were found in brown and black seeds, and their possible roles in regulating seed dormancy/germination were discussed.ConclusionsUpregulated genes involved in seed development and osmotic regulation substance accumulation may relate to bigger seed size and rapid seed germination in brown seeds, compared to black seeds. Differentially expressed genes of hormones may relate to seed dormancy/germination and the development of brown and black seeds. The transcriptome dataset will serve as a valuable resource to further understand gene expression and functional genomics in S. salsa dimorphic seeds.Electronic supplementary materialThe online version of this article (10.1186/s12864-017-4104-9) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Transcriptomic profiling of genes in matured dimorphic seeds of euhalophyte Suaeda salsa

Zhao

Duan

et al. 2017

BMC Genomics

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“…Inductively coupled plasma atomic emission spectrometry (ICP-AES) is a rapid and accurate technique for the simultaneous determination of the minor and major element contents [6][7][8]. In the digestion step the organic matter present in the seeds is destroyed in order to reduce interferences during the ICP-AES measurements.…”

Section: Introductionmentioning

confidence: 99%

ICP-AES Determination of Mineral Elements in Five Edible Seeds after Microwave Assisted Digestion

Wang

Qin

et al. 2014

AMR

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The concentrations of 12 elements (Ca, P, K, Na, Mg, Fe, Se, Mn, Zn, Sn, Cu and Al) were determined in raw and roasted pumpkin seeds by ICP-AES following microwave-assisted acid digestion. The accuracy of the method determined by spiking experiments was very good (recoveries 95.48103.2%) and the limits of detection of elements of interest were from 0.1 up to 17.4μg g-1, the relative standard deviations were less than 3.52%. Compared with watermelon seeds, winter melon seeds, sunflower seeds and hanging melon seeds, the results showed the five kinds of roasted melon seeds are all rich in mineral elements.

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“…and the heavy metals deposition can cause damaging effect on human system [1]. Most of the standard methods for determination of atmospheric heavy metals include ICP-MS [2][3][4][5], AAS [6][7], ICP-AES [8], etc. These methods often adopt an aerosol sampler with filters to collect particles in the air, and then the filters are used for lab analysis.…”

Section: Introductionmentioning

confidence: 99%

Development and Application of Continuous Atmospheric Heavy Metals Monitoring System Based on X-Ray Fluorescence

Liao

Guo

et al. 2012

AMR

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This paper presents a continuous atmospheric heavy metals monitoring system (AMMS-100) for heavy metals analysis based on X-ray fluorescence (XRF) technology. The AMMS-100 is used to collect the sample automatically with filters and then measure the concentrations of heavy metals by XRF. The accuracy of AMMS-100 for heavy metals analysis has been verified by the standard method. The relative errors, such as Pb and Fe, are lower than 10%. Moreover, the AMMS-100 can be used for long-term continuous monitoring the atmospheric heavy metals under different weather condition. The AMMS-100 described herein presents a continuous system for accurate analysis of atmospheric heavy metals with long-term stability.

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Study on Multi-Elements in Traditional Chinese Medicines <i>Ophiopogon japonicus</i> and Lotus Seeds by the ICP-AES with Microwave Digestion

Cited by 4 publications

References 27 publications

Transcriptomic profiling of genes in matured dimorphic seeds of euhalophyte Suaeda salsa

Transcriptomic profiling of genes in matured dimorphic seeds of euhalophyte Suaeda salsa

ICP-AES Determination of Mineral Elements in Five Edible Seeds after Microwave Assisted Digestion

Development and Application of Continuous Atmospheric Heavy Metals Monitoring System Based on X-Ray Fluorescence

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