Study on porosity of electrospun nanofiber membrane by neural network

Wang, Ting; Dong, Wenxia; Chen, Ying; Pan, Tiandi; Chen, Rudong

doi:10.12988/ams.2018.8582

Cited by 9 publications

(8 citation statements)

References 12 publications

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“…The porosity and surface area of fibrous mats dictate their breathability abilities for prospective uses in face masks. In electrospinning, porosity can be increased by raising the applied voltage [70]. Our data corroborate that premise; the incorporation of EOs within the PCL blends resulted in an almost 16.2-21.5% increase in porosity compared to PCL.…”

Section: Electrospun Mats Morphologysupporting

confidence: 87%

Inhibition of Escherichia Virus MS2, Surrogate of SARS-CoV-2, via Essential Oils-Loaded Electrospun Fibrous Mats: Increasing the Multifunctionality of Antivirus Protection Masks

et al. 2022

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One of the most important measures implemented to reduce SARS-CoV-2 transmission has been the use of face masks. Yet, most mask options available in the market display a passive action against the virus, not actively compromising its viability. Here, we propose to overcome this limitation by incorporating antiviral essential oils (EOs) within polycaprolactone (PCL) electrospun fibrous mats to be used as intermediate layers in individual protection masks. Twenty EOs selected based on their antimicrobial nature were examined for the first time against the Escherichia coli MS2 virus (potential surrogate of SARS-CoV-2). The most effective were the lemongrass (LGO), Niaouli (NO) and eucalyptus (ELO) with a virucidal concentration (VC) of 356.0, 365.2 and 586.0 mg/mL, respectively. PCL was processed via electrospinning, generating uniform, beadless fibrous mats. EOs loading was accomplished via two ways: (1) physisorption on pre-existing mats (PCLaEOs), and (2) EOs blending with the polymer solution prior to fiber electrospinning (PCLbEOs). In both cases, 10% v/v VC was used as loading concentration, so the mats’ stickiness and overwhelming smell could be prevented. The EOs presence and release from the mats were confirmed by UV-visible spectroscopy (≈5257–631 µg) and gas chromatography-mass spectrometry evaluations (average of ≈14.3% EOs release over 4 h), respectively. PCLbEOs mats were considered the more mechanically and thermally resilient, with LGO promoting the strongest bonds with PCL (PCLbLGO). On the other hand, PCLaNO and PCLaELO were deemed the least cohesive combinations. Mats modified with the EOs were all identified as superhydrophobic, capable of preventing droplet penetration. Air and water-vapor permeabilities were affected by the mats’ porosity (PCL < PCLaEOs < PCLbEOs), exhibiting a similar tendency of increasing with the increase of porosity. Antimicrobial testing revealed the mats’ ability to retain the virus (preventing infiltration) and to inhibit its action (log reduction averaging 1). The most effective combination against the MS2 viral particles was the PCLbLGO. These mats’ scent was also regarded as the most pleasant during sensory evaluation. Overall, data demonstrated the potential of these EOs-loaded PCL fibrous mats to work as COVID-19 active barriers for individual protection masks.

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Section: Electrospun Mats Morphologysupporting

confidence: 87%

Inhibition of Escherichia Virus MS2, Surrogate of SARS-CoV-2, via Essential Oils-Loaded Electrospun Fibrous Mats: Increasing the Multifunctionality of Antivirus Protection Masks

et al. 2022

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“…To measure the porosity of the nanofibers, the images were analyzed using the ImageJ software. [ 19,20 ] Eight‐bit sample images (400 × 400 pixels) were converted into binary images. The threshold value was identified, distinguishing between black and white pixels.…”

Section: Methodsmentioning

confidence: 99%

“…To measure the porosity of the nanofibers, the images were analyzed using the ImageJ software. [19,20] Eight-bit…”

Section: Confocal Laser Scanning Microscopy and Porosity Calculationmentioning

confidence: 99%

Control of mat thickness in electrospinning with transparent conductive glass collector

Nishiuchi

Tonami

2022

Polymer Engineering & Sci

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Electrospinning is a versatile technique for producing micro-and nanofibrous mats with great potential in many fields. However, reproducibility remains a key issue for product development. In particular, thickness is an important parameter for controlling electrospun fiber mats. In this study, we developed a method to control the polymer mat thickness during electrospinning. The mat thickness was controlled on the basis of the light transmittance and by using conductive indium tin oxide (ITO) glass connected to ground as a collector.Living cells were seeded on the fiber mat without detaching from the ITO glass, which enabled observation under a microscope and the evaluation of cell behavior. This study indicates that the thickness of the fiber mat can be consistently controlled by monitoring the light transmittance instead of the electrospinning duration and the mat can be easily observed under a microscope. This is expected to be particularly useful in the field of tissue engineering.

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“…Then, a histogram equalization (0.4% saturation) was performed and a threshold of 110 was set. Thus, porosity was calculated by using the following Formula (1) [ 22 ]:

…”

Section: Methodsmentioning

confidence: 99%

In Vitro Models for the Development of Peripheral Nerve Conduits, Part I: Design of a Fibrin Gel-Based Non-Contact Test

2021

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Current clinical strategies to repair peripheral nerve injuries draw on different approaches depending on the extent of lost tissue. Nerve guidance conduits (NGCs) are considered to be a promising, off-the-shelf alternative to autografts when modest gaps need to be repaired. Unfortunately, to date, the implantation of an NGC prevents the sacrifice of a healthy nerve at the price of suboptimal clinical performance. Despite the significant number of materials and fabrication strategies proposed, an ideal combination has not been yet identified. Validation and comparison of NGCs ultimately requires in vivo animal testing due to the lack of alternative models, but in the spirit of the 3R principles, a reliable in vitro model for preliminary screening is highly desirable. Nevertheless, more traditional in vitro tests, and direct cell seeding on the material in particular, are not representative of the actual regeneration scenario. Thus, we have designed a very simple set-up in the attempt to appreciate the relevant features of NGCs through in vitro testing, and we have verified its applicability using electrospun NGCs. To this aim, neural cells were encapsulated in a loose fibrin gel and enclosed within the NGC membrane. Different thicknesses and porosity values of two popular polymers (namely gelatin and polycaprolactone) were compared. Results indicate that, with specific implementation, the system might represent a useful tool to characterize crucial NGC design aspects.

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Study on porosity of electrospun nanofiber membrane by neural network

Cited by 9 publications

References 12 publications

Inhibition of Escherichia Virus MS2, Surrogate of SARS-CoV-2, via Essential Oils-Loaded Electrospun Fibrous Mats: Increasing the Multifunctionality of Antivirus Protection Masks

Inhibition of Escherichia Virus MS2, Surrogate of SARS-CoV-2, via Essential Oils-Loaded Electrospun Fibrous Mats: Increasing the Multifunctionality of Antivirus Protection Masks

Control of mat thickness in electrospinning with transparent conductive glass collector

In Vitro Models for the Development of Peripheral Nerve Conduits, Part I: Design of a Fibrin Gel-Based Non-Contact Test

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