Study on the antifungal mechanism of Chinese herbal extract on
            <i>Botryosphaeria dothidea</i>

Shi, Hao; Zhou, Xiangyu; XiaoE, He; Wang, Rencai; Zeng, Enni Liang; Zhou, Wen hua

doi:10.1111/jfpp.16631

Cited by 6 publications

(3 citation statements)

References 48 publications

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“…This is consistent with the antifungal mechanism of the Eriobotrya japonica leaf extract and the Chinese herbal extract compound (CHEC) on MDA. 67,68 The destruction of the cell membrane was also confirmed through the phenomenon of the mycelium being transparent and ruined by light microscopy. In the meantime, according to the increased transparency and breaking of the mycelium, we speculate that the petroleum ether extract may also do harm to the cell wall.…”

Section: ■ Discussionmentioning

confidence: 85%

Anti-Fungal Activity of Moutan cortex Extracts against Rice Sheath Blight (Rhizoctonia solani) and Its Action on the Pathogen’s Cell Membrane

Zhao

Wang

et al. 2022

ACS Omega

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Rice sheath blight (RSB) caused by Rhizoctonia solani is one of the most destructive diseases of rice (Oryza sativa). Although chemical fungicides are the most important control methods, their long-term unreasonable application has brought about problems such as environmental pollution, food risks, and non-target poisoning. Therefore, considering the extraction of fungistatic substances from plants may be an alternative in the future. In this study, we found that the Moutan cortex ethanol extract has excellent antifungal activity against R. solani, with a 100% inhibition rate at 1000 μg/mL, which aroused our great exploration interest. In-depth exploration found that the antifungal active ingredients of M. cortex were mainly concentrated in the petroleum ether extract of the M. cortex ethanol extract, which still maintained a 100% inhibition rate with 250 μg/mL, and its effective medium concentration (EC 50 ) was 145.33 μg/mL against R. solani. Through the measurement of extracellular relative conductivity and OD 260 , the petroleum ether extract induced leakage of intracellular electrolytes and nucleic acids, indicating that the cell membrane was ruined. Therefore, we preliminarily determined that the cell membrane may be the target of the petroleum ether extract. Moreover, we found that petroleum ether extract reduced the content of ergosterol, a component of the cell membrane, which may be one of the reasons for the cell membrane destruction. Furthermore, the increase of MDA content would lead to membrane lipid peroxidation, further aggravating membrane damage, resulting in increased membrane permeability. Also, the destruction of the cell membrane was observed by the phenomenon of the mycelium being transparent and broken. In conclusion, this is the first report of the M. cortex petroleum ether extract exhibiting excellent antifungal activity against R. solani. The effect of the M. cortex petroleum ether extract on R. solani may be on the cell membrane, inducing the disorder of intracellular substances and metabolism, which may be one of the antifungal mechanisms against R. solani.

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Section: ■ Discussionmentioning

confidence: 85%

Anti-Fungal Activity of Moutan cortex Extracts against Rice Sheath Blight (Rhizoctonia solani) and Its Action on the Pathogen’s Cell Membrane

Zhao

Wang

et al. 2022

ACS Omega

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“…Nevertheless, it can be observed that G. lucidum -based treatments exhibit some of the highest activities against the four phytopathogens. Regarding B. dothidea , the efficacy of the pure extract is comparable to that of a compound herbal extract compound consisting of seven Chinese medicinal plants [ 60 ]. Meanwhile, the activity of the conjugate complex is intermediate between those of COS- U. dioica and COS- E. arvense conjugates [ 41 ], tested against the same isolate.…”

Section: Discussionmentioning

confidence: 99%

Chemical Constituents and Antimicrobial Activity of a Ganoderma lucidum (Curtis.) P. Karst. Aqueous Ammonia Extract

Sánchez-Hernández

Teixeira

Pereira

et al. 2023

Plants

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Mushroom extracts have shown potential as a source of new antimicrobial agents. This study investigates the chemical profile of an aqueous ammonia extract obtained from the carpophores of Ganoderma lucidum, which grows on Quercus ilex trees, and explores its valorization as a biorational. The major chemical constituents of the extract, identified through gas chromatography–mass spectrometry, include acetamide, oleic acid, 1,2,3,4-butanetetrol, monomethyl azelate, undecane, and palmitic acid. The anti-oomycete and antifungal activity of G. lucidum extract was evaluated against Phytophthora cinnamomi, the primary threat to Quercus spp. in the dehesa biome, as well as three Botryosphaeriaceae fungi. In vitro tests revealed minimum inhibitory concentration (MIC) values of 187.5 μg·mL−1 against P. cinnamomi and 187.5–1000 μg·mL−1 against the fungi. Furthermore, conjugation of the G. lucidum extract with chitosan oligomers (COS) synergistically enhanced its antimicrobial activity, resulting in MIC values of 78.12 and 375–500 μg·mL−1 against P. cinnamomi and the fungi, respectively. These MIC values are among the highest reported to date for natural products against these phytopathogens. Subsequent ex situ testing of the COS-G. lucidum conjugate complex on artificially inoculated Q. ilex excised stems resulted in high protection against P. cinnamomi at a dose of 782 µg·mL−1. These findings support the potential utilization of this resource from the dehesa ecosystem to protect the holm oak, aligning with sustainable and circular economy approaches.

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“…DPPH scavenging capacity and SOD activity reflect antioxidant activities, which are associated with fungal adaptability to different environmental and stress conditions and host-infection ability [ 46 ]. Besides SOD, Shi et al [ 47 ] found that preservative treatment can reduce enzyme activities, including CAT, POD, NADP-malate dehydrogenase, succinate dehydrogenase, cellulase, and glucanase, in Bot. dothidea .…”

Section: Discussionmentioning

confidence: 99%

Exploring the Efficacy of Biocontrol Microbes against the Fungal Pathogen Botryosphaeria dothidea JNHT01 Isolated from Fresh Walnut Fruit

Zhang

Shi

Shen

et al. 2022

Foods

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Biological control by antagonistic microorganisms are an effective and environmentally friendly approach in postharvest disease management. In order to develop a biocontrol agent for fresh walnut fruit preservation, the potential biocontrol effects of Bacillus amyloliquefaciens RD.006 and Hanseniaspora uvarum FA.006 against the main fungal pathogen of walnuts were evaluated. Botryosphaeria species showed the highest detection, and the JNHT01 strain showed the strongest pathogenicity. Bot. dothidea JNHT01 caused gray mold and brown rot on fresh walnuts, and its incidence rate reached 100% after an 8 days incubation. The growth of this fungal strain can be promoted by lighting, with a maximum growth rate achieved at a pH of 7 and at 28 °C. B. amyloliquefaciens RD.006 and H. uvarum FA.006 supernatants at a concentration of 1–15% v/v showed antifungal activity. The mycelial growth inhibition rates of Bot. dothidea JNHT01 were 23.67–82.61% for B. amyloliquefaciens RD.006 and 1.45–21.74% for H. uvarum FA.006. During Bot. dothidea JNHT01 growth, the biomass, nucleic acid leakage, and malondialdehyde content gradually increased, while the DPPH scavenging capacity and SOD activity decreased. The B. amyloliquefaciens RD.006 and H. uvarum FA.006 strains showed antifungal activity by damaging fungal cell membranes and reducing fungal antioxidant activity. Moreover, the antifungal effect of B. amyloliquefaciens RD.006 was higher than that of H. uvarum FA.006. Hence, the RD.006 strain of B. amyloliquefaciens can be considered a potential biocontrol agent for the management of postharvest walnut diseases caused by Bot. dothidea.

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Study on the antifungal mechanism of Chinese herbal extract on Botryosphaeria dothidea

Cited by 6 publications

References 48 publications

Anti-Fungal Activity of Moutan cortex Extracts against Rice Sheath Blight (Rhizoctonia solani) and Its Action on the Pathogen’s Cell Membrane

Anti-Fungal Activity of Moutan cortex Extracts against Rice Sheath Blight (Rhizoctonia solani) and Its Action on the Pathogen’s Cell Membrane

Chemical Constituents and Antimicrobial Activity of a Ganoderma lucidum (Curtis.) P. Karst. Aqueous Ammonia Extract

Exploring the Efficacy of Biocontrol Microbes against the Fungal Pathogen Botryosphaeria dothidea JNHT01 Isolated from Fresh Walnut Fruit

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