Study on the effects of several operational variables on the enzymatic batch saccharification of orange solid waste

Torre, Isabel de la; Ravelo, Marianela; Segarra, Silvia; Tortajada, Marta; Santos, Victoria E.; Ladero, Miguel

doi:10.1016/j.biortech.2017.08.094

Cited by 39 publications

(14 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The OPW hydrolysates were prepared following the methodology described in Section 2.4. and developed by de la Torre and colleagues [22] obtaining a glucose yield around 60% w/w which corresponds to around 30 g L −1 , and obtaining a total sugar concentration above 50 g L −1 . Therefore, OPW is a good source of several monosaccharides but also have essential oils rich in limonene and containing terpenes and phenolics with some antimicrobial activity [21].…”

Section: Use Of Opw Hydrolysate For D-la Production By Selected Strainsmentioning

confidence: 96%

“…The results obtained by applying the NREL methodology are compiled in Table 2. For D-LA production assays, OPW was milled down to 1-2 mm particle size and hydrolysis was carried out at 10% w/w of dry solid, 50 • C, 300 rpm and initial pH of 5.2 using enzyme cocktails with cellulases, β-glucosidase, xylanase, β-xylosidase, pectinase, and auxiliary activities (Celluclast 1.5 l, Novozym 188, Pectinex Ultra SP-L gifted by Novozymes) as described by de la Torre and colleagues [22].…”

Section: Opw Pretreatmentsmentioning

confidence: 99%

“…In this sense, the valorization of food waste to useful products such as D-LA is a good alternative [1,18,19]. In particular, orange peel and pulp waste (OPW) can be used to produce D-LA after adequate pre-treatment processes [20][21][22].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Production of D-Lactic Acid by the Fermentation of Orange Peel Waste Hydrolysate by Lactic Acid Bacteria

et al. 2019

Self Cite

View full text Add to dashboard Cite

Lactic acid is one the most interesting monomer candidates to replace some petroleum- based monomers. The application of conventional poly-lactic acid (PLA) is limited due to insufficient thermal properties. This limitation can be overcome by blending poly-D and poly-L-lactic acid. The main problem is the limited knowledge of D-lactic acid (D-LA) production. Efficient biochemical processes are being developed in order to synthesize D-LA from orange peel waste (OPW). OPW is an interesting renewable raw material for biorefinery processes of biocatalytic, catalytic or thermal nature owing to its low lignin and ash content. Bioprocessing of the pretreated OPW is carried out by enzymatic hydrolysis and fermentation of the released sugars to produce D-LA. Several strains of the species Lactobacillus delbrueckii ssp. bulgaricus have been evaluated for the production of D-LA from OPW hydrolysate using Lactobacillus delbrueckii ssp. delbrueckii CECT 286 as a reference strain since its performance in this kind of substrate have been widely reported in previous studies. Preliminary results show that Lactobacillus delbrueckii ssp. bulgaricus CECT 5037 had the best performance with a yield of 84% w/w for D-LA production and up to 95% (e.e.).

show abstract

Section: Use Of Opw Hydrolysate For D-la Production By Selected Strainsmentioning

confidence: 96%

Section: Opw Pretreatmentsmentioning

confidence: 99%

See 1 more Smart Citation

Production of D-Lactic Acid by the Fermentation of Orange Peel Waste Hydrolysate by Lactic Acid Bacteria

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…On the other hand, orange juice processing waste consists of high levels of lignin, cellulose and hemicellulose, which has been reported to be equal to 40% of the dry weight [3]. A range of biotechnological applications have recently been proposed for the valorization of orange juice processing waste, including the recovery of high added-value products like natural antioxidants, essential oils and pectin [4,5], the manufacturing of absorbent material [6] and the production of bioethanol and biomethane [7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Diversity and Biotechnological Potential of Xylan-Degrading Microorganisms from Orange Juice Processing Waste

Zerva

Remmas

Ntougias

2019

Water

View full text Add to dashboard Cite

The orange juice processing sector produces worldwide massive amounts of waste, which is characterized by high lignin, cellulose and hemicellulose content, and which exceeds 40% of the fruit’s dry weight (d.w.). In this work, the diversity and the biotechnological potential of xylan-degrading microbiota in orange juice processing waste were investigated through the implementation of an enrichment isolation strategy followed by enzyme assays for the determination of xylanolytic activities, and via next generation sequencing for microbial diversity identification. Intracellular rather than extracellular endo-1,4-β-xylanase activities were detected, indicating that peripheral cell-bound (surface) xylanases are involved in xylan hydrolysis by the examined microbial strains. Among the isolated microbial strains, bacterial isolates belonging to Pseudomonas psychrotolerans/P. oryzihabitans spectrum (99.9%/99.8% similarity, respectively) exhibited activities of 280 U/mg protein. In contrast, almost all microbial strains isolated exerted low extracellular 1,4-β-xylosidase activities (<5 U/mg protein), whereas no intracellular 1,4-β-xylosidase activities were detected for any of them. Illumina data showed the dominance of lactic and acetic acid bacteria and of the yeasts Hanseniaspora and Zygosaccharomyces. This is the first report on indigenous xylanolytic microbiota isolated from orange juice processing waste, possessing the biotechnological potential to serve as biocatalysts for citrus biomass valorization through the production of high-added value products and energy recovery.

show abstract

“…Adicionalmente, outros autores mostraram que o controle do pH também está relacionado ao sucesso da sacarificação, possivelmente por favorecer a ação de enzimas importantes para o processo (LA TORRE et al, 2017 Fonte: Elaborado pela autora.…”

Section: Caseína Farinha De Penaunclassified

Estudo da alteração do perfil de proteínas de fungos filamentosos em diferentes condições de cultivo utilizando ferramentas proteômicas e ensaios enzimáticos

Garzon¹

View full text Add to dashboard Cite

Study on the effects of several operational variables on the enzymatic batch saccharification of orange solid waste

Cited by 39 publications

References 44 publications

Production of D-Lactic Acid by the Fermentation of Orange Peel Waste Hydrolysate by Lactic Acid Bacteria

Production of D-Lactic Acid by the Fermentation of Orange Peel Waste Hydrolysate by Lactic Acid Bacteria

Diversity and Biotechnological Potential of Xylan-Degrading Microorganisms from Orange Juice Processing Waste

Estudo da alteração do perfil de proteínas de fungos filamentosos em diferentes condições de cultivo utilizando ferramentas proteômicas e ensaios enzimáticos

Contact Info

Product

Resources

About