Study on the killing effect of cold atmospheric pressure plasma on MRSA Staphylococcus aureus in vitro and in vivo infection model

Namini, Yasaman Nazar; Heidarzadeh, Siamak; Khaledi, Azad; Abbasi, Elham; Abbasi, Awais Saeed; Esmaeili, Davood

doi:10.21161/mjm.180295

Cited by 4 publications

(1 citation statement)

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“…CAP is highly effective in eradicating multi-drug resistant bacteria in vitro and in vivo , such as isolates of methicillin-resistant Staphylococcus aureus (MRSA) ( 44 , 54 , 55 ). This is in concordance with findings in our study showing that the treatment of two methicillin-resistant isolates of S. pseudintermedius achieved very similar results compared to methicillin-sensitive isolates.…”

Section: Discussionmentioning

confidence: 99%

Argon cold atmospheric plasma eradicates pathogens in vitro that are commonly associated with canine bacterial keratitis

Marx,

Oltmanns,

Meißner

et al. 2024

Front. Vet. Sci.

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PurposeTo investigate the antimicrobial effect of cold atmospheric plasma (CAP) on pathogens associated with canine bacterial keratitis.Materials and methodsPseudomonas aeruginosa, Staphylococcus pseudintermedius, and Streptococcus canis strains, which were obtained from dogs with infectious keratitis, were subjected to testing. For each species, four isolates and a reference strain were cultivated on Columbia sheep blood agar and treated with the kiNPen Vet® plasma pen from Neoplas GmbH, Greifswald, Germany. Various continuous treatment durations (0.5, 2, and 5 min) were applied, along with a 0.5-min treatment repeated four times at short intervals. These treatments were conducted at distances of 3 and 18 mm between the agar surface and the pen.ResultsCAP treatment reduced bacterial growth in all three species. The most effective treatment duration was 5 min at 3 mm distance, resulting in inhibition zones ranging from 19 to 22 mm for P. aeruginosa, 26–45 mm for S. pseudintermedius and an overall reduction of bacterial growth for Str. canis. Inhibition zones were smaller with decreasing treatment duration and larger distance. Treatment times of 30 s repeated four times and 2 min showed comparable results. Treatment with argon alone did not lead to visible reduction of bacterial growth.ConclusionArgon cold atmospheric plasma demonstrated a potent in vitro antimicrobial effect on P. aeruginosa, S. pseudintermedius and Str. canis strains with the latter showing the highest sensitivity.

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Section: Discussionmentioning

confidence: 99%