Study on the Mechanism of Arsenic-Induced Lung Injury Based on SWATH Proteomics Technology

Wang, Yi; Zhang, Jun; Zhang, Xinglai; Zhang, Huajie; Cao, Xueshan; Hu, Ting; Jing, Lin; Tang, Xiaoxiao; Chen, Xiaolu; Jiang, Yuxuan; Yan, Xi; Zhuang, Hongbin; Luo, Peng; Shen, Liming

doi:10.1007/s12011-022-03466-2

Cited by 7 publications

(7 citation statements)

References 67 publications

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“…SWATH‐MS analysis was performed as described in previous studies 63,64 . In information‐dependent acquisition (IDA) analysis, 3 μg peptide of each batch was injected for eluting that used a gradient elution with solvents A and B at 5 μL/min for 60 min.…”

Section: Methodsmentioning

confidence: 99%

“…A filter‐aided sample preparation method was employed in‐solution protein digestion 62 . Approximately 100 μg of proteins from each sample were reduced, alkylated, and digested with trypsin (Promega) as described previously 63,64 . Centrifugation was used to separate the peptides for 15 min at 12,000 g, 4°C.…”

Section: Methodsmentioning

confidence: 99%

“…SWATH‐MS analysis was performed as described in previous studies. 63 , 64 In information‐dependent acquisition (IDA) analysis, 3 μg peptide of each batch was injected for eluting that used a gradient elution with solvents A and B at 5 μL/min for 60 min. Based on IDA raw data, 75 variable Q1 isolation window scheme was calculated by SWATH Variable Window Assay Calculator V1.1.…”

Section: Methodsmentioning

confidence: 99%

“…62 Approximately 100 μg of proteins from each sample were reduced, alkylated, and digested with trypsin (Promega) as described previously. 63,64 Centrifugation was used to separate the peptides for 15 min at 12,000 g, 4 • C. Following reverse-phase chromatography (Durashell, C18, 250 × 4.6 mm, 5 μm; Bonna-Agela Technologies Inc.) at high pH, the peptides (400 μg) were subjected to salt removal using an Agilent HPLC system (Agilent Technologies). The peptides were then lyophilized and kept at 80 • C after being eluted and mixed into 30 batches.…”

Section: Protein Sample Preparationmentioning

confidence: 99%

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A study of genetic heterogeneity in autism spectrum disorders based on plasma proteomic and metabolomic analysis: multiomics study of autism heterogeneity

Tang,

Feng,

Zhao

et al. 2023

MedComm

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Genetic heterogeneity poses a challenge to research and clinical translation of autism spectrum disorder (ASD). In this study, we conducted a plasma proteomic and metabolomic study of children with ASD with and without risk genes (de novo mutation) and controls to explore the impact of genetic heterogeneity on the search for biomarkers for ASD. In terms of the proteomic and metabolomic profiles, the groups of children with ASD carrying and those not carrying de novo mutation tended to cluster and overlap, and integrating them yielded differentially expressed proteins and differential metabolites that effectively distinguished ASD from controls. The mechanisms associated with them focus on several common and previously reported mechanisms. Proteomics results highlight the role of complement, inflammation and immunity, and cell adhesion. The main pathways of metabolic perturbations include amino acid, vitamin, glycerophospholipid, tryptophan, and glutamates metabolic pathways and solute carriers‐related pathways. Integrating the two omics analyses revealed that L‐glutamic acid and malate dehydrogenase may play key roles in the pathogenesis of ASD. These results suggest that children with ASD may have important underlying common mechanisms. They are not only potential therapeutic targets for ASD but also important contributors to the study of biomarkers for the disease.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Protein Sample Preparationmentioning

confidence: 99%

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A study of genetic heterogeneity in autism spectrum disorders based on plasma proteomic and metabolomic analysis: multiomics study of autism heterogeneity

Tang,

Feng,

Zhao

et al. 2023

MedComm

Self Cite

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show abstract

“…Previous studies have shown that As has the property of accumulating in tissues. In animal experiments with arsenate or arsenite, the concentration of As in different tissues and organs of animals increases, such as kidney, liver, lung and spleen [33][34][35]. As is neurotoxic and causes CNS dysfunction, such as cognitive dysfunction, behavioral de cits and mood disorders [36].…”

Section: Discussionmentioning

confidence: 99%

Effects of arsenic exposure on trace element levels in the hippocampus and cortex of rats and their gender differences

Yan,

Zhang,

et al. 2023

Journal of Trace Elements in Medicine and Biology

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Study on the mechanism of arsenic-induced renal injury based on SWATH proteomics technology

Chen,

Yan,

Tang

et al. 2024

Journal of Trace Elements in Medicine and Biology

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Study on the Mechanism of Arsenic-Induced Lung Injury Based on SWATH Proteomics Technology

Cited by 7 publications

References 67 publications

A study of genetic heterogeneity in autism spectrum disorders based on plasma proteomic and metabolomic analysis: multiomics study of autism heterogeneity

A study of genetic heterogeneity in autism spectrum disorders based on plasma proteomic and metabolomic analysis: multiomics study of autism heterogeneity

Effects of arsenic exposure on trace element levels in the hippocampus and cortex of rats and their gender differences

Study on the mechanism of arsenic-induced renal injury based on SWATH proteomics technology

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