Study on the role of patulin on pathogenicity and virulence of Penicillium expansum

Sanzani, Simona Marianna; Reverberi, Massimo; Punelli, Marta; Ippolito, Antonio; Fanelli, C.

doi:10.1016/j.ijfoodmicro.2011.11.021

Cited by 120 publications

(79 citation statements)

References 36 publications

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“…38) In addition, P. expansum mutants that show reduced transcription of msas produced reduced amounts of patulin. 39) Our results strongly suggest that the step catalyzed by the 6-MSA synthase might be one of the rate-determining steps in patulin biosynthesis, and might prove an effective target in an effort to control patulin production.…”

Section: Discussionmentioning

confidence: 59%

Effects of Aliphatic Aldehydes on the Growth and Patulin Production ofPenicillium expansumin Apple Juice

Taguchi¹,

Kozutsumi²,

Nakamura³

et al. 2013

Bioscience, Biotechnology, and Biochemistry

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Section: Discussionmentioning

confidence: 59%

Effects of Aliphatic Aldehydes on the Growth and Patulin Production ofPenicillium expansumin Apple Juice

Taguchi¹,

Kozutsumi²,

Nakamura³

et al. 2013

Bioscience, Biotechnology, and Biochemistry

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“…Application of isolated AOH on wounded tomato tissue leads to a distinct opening of the wound which would facilitate the colonization by the fungus. Interestingly Sanzani et al (2012) very recently demonstrated in a similar approach, that patulin produced by P. expansum plays a role as pathogenicity factor.…”

Section: Discussionmentioning

confidence: 98%

HOG MAP kinase regulation of alternariol biosynthesis in Alternaria alternata is important for substrate colonization

Graf

Schmidt‐Heydt

Geisen

2012

International Journal of Food Microbiology

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“…These authors showed that the BCA R. kratochvilovae LS11, a basidiomycete red yeast belonging to the subphylum Pucciniomycotina, was able to resist and degrade a high concentration of patulin both in vitro and in a model system mimicking P. expansum-infected apple tissue (8,14). Intriguingly, it has been recently reported that patulin is a pathogenicity factor of P. expansum (15), suggesting that patulin degradation may itself be considered a mechanism of biocontrol.…”

mentioning

confidence: 99%

Searching for Genes Responsible for Patulin Degradation in a Biocontrol Yeast Provides Insight into the Basis for Resistance to This Mycotoxin

Ianiri

Idnurm

Wright

et al. 2013

Appl Environ Microbiol

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f Patulin is a mycotoxin that contaminates pome fruits and derived products worldwide. Basidiomycete yeasts belonging to the subphylum Pucciniomycotina have been identified to have the ability to degrade this molecule efficiently and have been explored through different approaches to understand this degradation process. In this study, Sporobolomyces sp. strain IAM 13481 was found to be able to degrade patulin to form two different breakdown products, desoxypatulinic acid and (Z)-ascladiol. To gain insight into the genetic basis of tolerance and degradation of patulin, more than 3,000 transfer DNA (T-DNA) insertional mutants were generated in strain IAM 13481 and screened for the inability to degrade patulin using a bioassay based on the sensitivity of Escherichia coli to patulin. Thirteen mutants showing reduced growth in the presence of patulin were isolated and further characterized. Genes disrupted in patulin-sensitive mutants included homologs of Saccharomyces cerevisiae YCK2, PAC2, DAL5, and VPS8. The patulin-sensitive mutants also exhibited hypersensitivity to reactive oxygen species as well as genotoxic and cell wall-destabilizing agents, suggesting that the inactivated genes are essential for tolerating and overcoming the initial toxicity of patulin. These results support a model whereby patulin degradation occurs through a multistep process that includes an initial tolerance to patulin that utilizes processes common to other external stresses, followed by two separate pathways for degradation.

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Study on the role of patulin on pathogenicity and virulence of Penicillium expansum

Cited by 120 publications

References 36 publications

Effects of Aliphatic Aldehydes on the Growth and Patulin Production ofPenicillium expansumin Apple Juice

Effects of Aliphatic Aldehydes on the Growth and Patulin Production ofPenicillium expansumin Apple Juice

HOG MAP kinase regulation of alternariol biosynthesis in Alternaria alternata is important for substrate colonization

Searching for Genes Responsible for Patulin Degradation in a Biocontrol Yeast Provides Insight into the Basis for Resistance to This Mycotoxin

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