Study on the transcriptional regulatory mechanism of the MyoD1 gene in Guanling bovine

Zhou, Di; Xu, Houqiang; Chen, Wei; Wang, Yuanyuan; Zhang, Ming; Yang, Tao

doi:10.1039/c7ra11795g

Cited by 4 publications

(3 citation statements)

References 48 publications

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“…surrounding 200 kb) to the significant SNPs affecting Nellore age at first calving [ 27 ]. Also, the MYOD1 gene is a component of myogenic regulatory factors involved in myoblast differentiation and in concordance with [ 28 , 29 ] results, this gene may potentially affecting meat production.…”

Section: Discussionmentioning

confidence: 60%

A genome-wide scan of copy number variants in three Iranian indigenous river buffaloes

et al. 2021

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Background In Iran, river buffalo is of great importance. It plays an important role in the economy of the Country, because its adaptation to harsh climate conditions and long productive lifespan permitting its farming across the Country and to convert low-quality feed into valuable milk. The genetic variability in Iranian buffalo breeds have been recently studied using SNPs genotyping data, but a whole genome Copy Number Variants (CNVs) mapping was not available. The aim of this study was to perform a genome wide CNV scan in 361 buffaloes of the three Iranian river breeds (Azeri, Khuzestani and Mazandarani) through the analysis of data obtained using the Axiom® Buffalo Genotyping Array 90 K. Results CNVs detection resulted in a total of 9550 CNVs and 302 CNVRs identified in at least 5% of samples within breed, covering around 1.97% of the buffalo genome. and A total of 22 CNVRs were identified in all breeds and a different proportion of regions were in common among the three populations. Within the more represented CNVRs (n = 302) mapped a total of 409 buffalo genes, some of which resulted associated with morphological, healthy, milk, meat and reproductive traits, according to Animal Genome Cattle database. Conclusions This work provides a step forward in the interpretation of genomic variation within and among the buffalo populations, releasing a first map of CNVs and providing insights about their recent selection and adaptation to environment. The presence of the set of genes and QTL traits harbored in the CNVRs could be possibly linked with the buffalo’s natural adaptive history together to a recent selection for milk used as primary food source from this species.

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Section: Discussionmentioning

confidence: 60%

A genome-wide scan of copy number variants in three Iranian indigenous river buffaloes

et al. 2021

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“…MyoD1 , as a member of its family, is a major transcriptional regulator of muscle-specific genes, and its activity is directly related to muscle growth. Although previous studies related to the promoter binding site of the MyoD1 gene in Guanling cattle have been reported [ 12 , 21 , 22 ], the mechanism of action of the bovine MyoD1 gene on muscle cells has rarely been explored. In view of the cell growth arrest caused by MyoD1 knockdown in muscle cells in previous experiments, in the present study, after knockdown of the MyoD1 gene in bovine kidney cells, we discovered that PDE1B, histone 3.1, ADAMTS1, DPT, and CYP4F2 were upregulated, implying they may be a compensatory pathway after MyoD1 knockdown.…”

Section: Discussionmentioning

confidence: 99%

“…Guanling cattle are a local fine breed in Guizhou Province, one of the 78 local livestock breeds under national key protection, ranking first among the four major yellow cattle in Guizhou with a high reproduction rate, high slaughter rate, high meat yield, high amino acid content, high protein content, low fat content, and other characteristics, with high food value, economic value, and development value. Previous studies of Guanling cattle have utilized promoter and transcription factor analyses combined with yeast one-hybrid systems to demonstrate that Myf6 strongly activated the MyoD1 promoter [ 12 ]. However, the role of MyoD1 in muscle differentiation is unclear.…”

Section: Introductionmentioning

confidence: 99%

The MyoD1 Promoted Muscle Differentiation and Generation by Activating CCND2 in Guanling Cattle

Zhou¹,

Wang²,

Yang³

et al. 2022

Animals

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The purpose of this study was to analyze the transcriptome of MyoD1 gene knockout MDBK cells (bovine kidney cells) using high-throughput sequencing. For the first time, CRISPR/CAS9 technology was used to construct a MyoD1 knockout in MDBK cells and transcriptome sequence analysis was used to examine MyoD1-related target gene expression. Transcriptome sequencing indicated the presence of 723 differentially expressed genes (DEGs) by comparing wild type and MyoD1 knockout MDBK cells and included 178 upregulated and 72 downregulated genes. The DEGs are mainly enriched in Pl-3-kinase and AKT, p53 signaling pathways. Quantitative RT-PCR confirmed that PDE1B, ADAMTS1, DPT, and CCND2 were highly expressed in the leg muscle, longissimus dorsi, and shoulder of Guanling cattle, and CCND2 was inhibited after MyoD1 knockout, suggesting it may be a key downstream gene of MyoD1 and associated with muscle formation and differentiation in Guanling cattle. This provides experimental data for subsequent studies on the regulatory mechanisms of muscle differentiation in Guanling cattle.

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MicroRNA, mRNA and protein responses to dehydration in skeletal muscle of the African-clawed frog, Xenopus laevis

2022

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Study on the transcriptional regulatory mechanism of the MyoD1 gene in Guanling bovine

Abstract: The MyoD1 gene plays a key role in regulating the myoblast differentiation process in the early stage of skeletal muscle development.

Cited by 4 publications

References 48 publications

A genome-wide scan of copy number variants in three Iranian indigenous river buffaloes

A genome-wide scan of copy number variants in three Iranian indigenous river buffaloes

The MyoD1 Promoted Muscle Differentiation and Generation by Activating CCND2 in Guanling Cattle

MicroRNA, mRNA and protein responses to dehydration in skeletal muscle of the African-clawed frog, Xenopus laevis

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