This paper presents a critical review on the measuring methods and parameters affecting nano-tribology in the context of nano-scale wear. Nano-scale wear phenomena play a crucial role in various industries, including micro/nano-systems, electronics, and biotechnology. The review begins by discussing the significance of nano-scale wear and its impact on device performance, lifespan, durability, energy efficiency, cost savings, and environmental sustainability. It then delves into the measuring methods employed to assess nano-scale wear, including scanning probe microscopy (SPM) techniques such as atomic force microscopy (AFM) and friction force microscopy (FFM). The capabilities of AFM and FFM in studying the roughness of surface, adhesion, friction, scratch, abrasion, and nano-scale material transfer are highlighted. Additionally, the review explores the parameters affecting nano-wear, such as lubrication strategies, stress levels, sliding velocity, and atomic-scale reactions. The article concludes by emphasizing the importance of advanced microscopy techniques in understanding tribological mechanisms at different scales, bridging the gap between macro and nano-tribology studies.