Study the Anti-Toxoplasma Antibodies (IgG and IgM) in Hemodialysis Patients of Abadan and Khoramshahr Cities Southwest Iran in 2011 using ELISA

Abstract

Background: Toxoplasmosis is a worldwide disease caused by an obligate intracellular protozoan, Toxoplasma gondii and can cause severe infections in immune-compromised individuals. Objectives: The aim of this study was to determinate the anti-Toxoplasma antibodies in hemodialysis patients of Abadan and Khoramshahr, Southwest of Iran. Materials and Methods: Sera of 150 patients (test group) aged 21 to 87 years referred regularly to hemodialysis departments in Abadan and Khoramshahr cities, and 150 healthy individuals (control group) were examined for anti-Toxoplasma (IgG and IgM) antibodies using ELISA kits and the results were analyzed using Chi-square and fisher exact test. Results: 61 (40.67%) out of 150 sera of patients were positive, 6 (4%) were borderline and 83 (55.33%) were negative for anti-Toxoplasma IgG. For anti-Toxoplasma IgM, 13 (8.67%) of 150 were positive, 21 (14%) were borderline and 116 (77.33%) were negative. In control group 39 (26%) of 150 individuals were positive, 14 (9.33%) were borderline and 97 (64.67%) were negative for anti-Toxoplasma IgG. In the sera of individuals in control group, anti-Toxoplasma IgM was not detected. In hemodialysis patients, 7 (4.66%) cases were positive for anti-Toxoplasma IgG and IgM and 8 (5.33%) cases were IgG positive and IgM borderline. There were significant differences in IgG and IgM level of the test and control groups (P ˂ 0.05) Conclusions: Hemodialysis patients are high risk group for toxoplasmosis and should be tested periodically to prevent the dissemination of toxoplasmosis during dialysis.

Serological and molecular survey of toxoplasmosis in renal transplant recipients and hemodialysis patients in Kashan and Qom regions, central Iran

“…[13][14][15][16][17][18][19][20] Moreover, higher seroprevalence of toxoplasmosis has been reported in hemodialysis and chronic renal failure patients. 12,23,25,32 The diagnosis of toxoplasmosis is based on serology. If an individual was IgM or IgM þ IgG positive, the patient has an active infection.…”

“…T. gondii IgM seropositivity was detected in 8.6% of HD patients and none of the control group. 25 The diagnosis of active T. gondii infection by molecular methods is more sensitive than serological tests. 33,34 In the current study, T. gondii DNA was detected in 6 of 50 (12%) RTR patients.…”

“…24 Maraghi et al 25 found that T. gondii IgG and IgM antibodies were significantly elevated in HD patients than the control group. According to the results, 25 T. gondii IgG was detected in 40.67% and 26% of the case and control groups, respectively. T. gondii IgM seropositivity was detected in 8.6% of HD patients and none of the control group.…”

“…T. gondii IgM antibody was also detected in 13.5% of the case and none of the control group. 24 Maraghi et al 25 found that T. gondii IgG and IgM antibodies were significantly elevated in HD patients than the control group. According to the results, 25 T. gondii IgG was detected in 40.67% and 26% of the case and control groups, respectively.…”

“…[23][24][25] Iran is one of the pioneering countries in hemodialysis and renal transplantation. 26,27 While the first case of renal transplantation took place in 1967, the number of transplantation has been dramatically increased until now.…”