Studying of Isotopic Effects of Deuterium in Biological Objects

Mosin, Oleg; Ignatov, Ignat

doi:10.13187/ercr.2015.3.25

Cited by 5 publications

(6 citation statements)

References 16 publications

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“…However, it is known for decades that D 2 O directly interferes with the cell cycle, markedly slows down the overall gene expression, and reduces the proliferation of various cell types, which has proven a possibility to impair human tumor growth in animal models. [1][2][3][4][5][6][7] D 2 O is the only known chemical substance that affects the period of circadian oscillations, consistently increasing the length of each cycle. [8] Generally, heavy water or deuterium oxide (D 2 O) is a form of water, where both protium atoms ( 1 H-only one proton) of normal H 2 O are replaced by the hydrogen isotope deuterium ( 2 H-one proton + one neutron).…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cells in Slow Motion: Apparent Undercooling Increases Glassy Behavior at Physiological Temperatures

et al. 2021

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Solvent conditions are unexpectedly sufficient to drastically and reversibly slow down cells. In vitro on the molecular level, protein–solvent interactions drastically change in the presence of heavy water (D2O) and its stronger hydrogen bonds. Adding D2O to the cell medium of living cells increases the molecular intracellular viscosity. While cell morphology and phenotype remain unchanged, cellular dynamics transform into slow motion in a changeable manner. This is exemplified in the slowdown of cell proliferation and migration, which is caused by a reversible gelation of the cytoplasm. In analogy to the time–temperature superposition principle, where temperature is replaced by D2O, an increase in viscosity slows down the effective time. Actin networks, crucial structures in the cytoplasm, switch from a power‐law‐like viscoelastic to a more rubber‐like elastic behavior. The resulting intracellular resistance and dissipation impair cell movement. Since cells are highly adaptive non‐equilibrium systems, they usually respond irreversibly from a thermodynamic perspective. D2O induced changes, however, are fully reversible and their effects are independent of signaling as well as expression. The stronger hydrogen bonds lead to glass‐like, drawn‐out intramolecular dynamics, which may facilitate longer storage times of biological matter, for instance, during transport of organ transplants.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cells in Slow Motion: Apparent Undercooling Increases Glassy Behavior at Physiological Temperatures

et al. 2021

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“…Another logical explanation for the observed effect, if we take into account the origin of leucine and isoleucine due to biosynthesis in various pathways (leucine belongs to the family of pyruvate, while isoleucine -to the family of aspartate), could be assimilation by the cell of the unlabeled leucine from the growth media under the background biosynthesis of isotopic-labeled isoleucine de novo. Taking into account these effects it should be emphasized that the use of auxotrophic forms of microorganisms for production isotope-labeled amino acids could not be justified practically because of the multiple character of inclusion of isotopes into the molecule [20]. On the contrary, the use for this purpose the prototrophic forms of microrganisms seem to be more promising for these aims.…”

Section: Study Of Levels Of Inclusion Of Stable Isotopes Of 2 н and 1mentioning

confidence: 99%

To the question of mobility of particles and molecules in porous media

Sharfarets¹,

Курочкин²

2015

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The work demonstrates the possibility of using electron impact mass spectrometry (EI) on a MB-80A device ("Hitachi", Japan) with a double electron focusing for analysis of [ 2 H, 13 C]amino acid mixtures of L-phenylalanine producing strain of Brevibacterium methylicum and L-leucine-producing strain of Methylobacillus flagellatum, and [ 2 H, 13 C]amino acids of the total biomass protein isolated while growing the bacterial cells on media containing as a source of stable isotopes [ 2 H]methanol, [ 13 C] methanol and 2 H 2 O. For mass-spectrometric analysis of the level of incorporation of stable isotopes 2 H and 13 C into the molecules of [ 2 H, 13 C]amino acids the multi-componential mixtures of [ 2 H, 13 C]amino acids, derived from cultural media and protein hydrolysates after hydrolysis in 6 M 2 HСl (3 % phenol) and 2 M Ва(OH) 2 were modified into N-benzyloxycarbonyl-derivatives of [ 2 H, 13 C]amino acids as well as into the methyl esters of N-dansyl-derivatives of [ 2 H, 13 C]amino acids, which were separated by the method of reverse phase high performance liquid chromatography (RP HPLC) on a column with octadecylsilane gel Separon SGX C18. The levels of 2 H and 13 С enrichment of secreted amino acids and amino acid residues of protein were found to be varied depending on the metabolic pathways of biosynthesis and concentration of 2 Hand 13 C-labelled substrates in growth media from 20.0 atom. % to L-leucine/isoleucine up to 97.5 atom. % for L-alanine.

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“…The assimilation rate of methylotrophic biomass by prokaryotic and eukaryotic cells makes up 85-98 %, and their productivity calculated on the level of methanol bioconversion into cell components reaches 50 % [12]. As we have earlier reported, methylotrophic bacteria are convenient objects capable to grow on minimal salt media containing 2-4 % [ 2 H]methanol, whereon other bacteria are unable to reproduce, and may easily be adapted to maximal 2 Н 2 О concentrations [13]. However, the experiments discovered a bacteriostatic effect of 2 Н 2 О consisted in inhibition of vitally important cell functions of the bacterial producer cells [14].…”

Section: Introductionmentioning

confidence: 99%

Сreating a precision circuits for discharge of charge integrator for use in spectrometric devices for observations in the far space

Lisin¹,

Lebedev²

2016

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In this paper was demonstrated the possibility of the use of FAB mass spectrometry on an impulse mass spectrometer VG-70 SEQ ("Fisons VG Analytical", USA) for the study of biosynthetic pathways of 2 H-labeled purine ribonucleoside inosine secreted into the liquid culture (LC) by Gram-positive chemoheterotrophic bacterium Bacillus subtilis VKPM B-3157 when grown on heavy water (HW) medium with 2 % hydrolyzate of deuterated biomass of methylotrophic bacterium Brevibacterium methylicum VKPM B-5662 as a source of growth 2 H-labeled substrates. Isolation of 2 H-labeled inosine from the LC was performed by adsorption/desorption on activated carbon with following extraction by 0.3 M ammonium-formate buffer (pH = 8.9), crystallization in 80 % ethanol and ion exchange chromatography (IEC) on a column with AG50WX 4 cation exchange resin equilibrated with 0.3 M ammonium-formate buffer and 0.045 M NH 4 Cl. The investigation of deuterium incorporation into the molecule of biosynthetic [ 2 H]inosine by FAB mass spectrometry demonstrated the incorporation of 5 deuterium atoms into the molecule (the total level of deuterium enrichment -62.5 atom. % 2 H) with incorporation of 3 deuterium atoms into the ribose and 2 deuterium atoms -into the hypoxanthine residue of the molecule. Three non-exchangeable deuterium atoms were incorporated into the ribose residue owing to reactions of enzymatic izomerization of glucose in 2 H 2 O-medium due to reactions of glycolysis, associated with the Embden-Meyerhof pathway with participation of reactions of isotope ( 1 Н-2 Н) exchange, while two other deuterium atoms at C2, C8-positions in the hypoxanthine residue were synthesized from [ 2 H]amino acids that originated from the deuterated hydrolysate of the methylotrophic bacterium Brevibacterium methylicum VKPM B-5662. named after M. V. Lomonosov, Russia (Shvets V. I.) Moscow State University of Fine Chemical Technology

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Studying of Isotopic Effects of Deuterium in Biological Objects

Cited by 5 publications

References 16 publications

Cells in Slow Motion: Apparent Undercooling Increases Glassy Behavior at Physiological Temperatures

Cells in Slow Motion: Apparent Undercooling Increases Glassy Behavior at Physiological Temperatures

To the question of mobility of particles and molecules in porous media

Сreating a precision circuits for discharge of charge integrator for use in spectrometric devices for observations in the far space

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