Studying Plaque Biofilms on Various Dental Surfaces

Steinberg, Doron

doi:10.1385/1-59259-224-4:353

Cited by 41 publications

(55 citation statements)

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“…Surface proteins that cause oral bacterial aggregation include glucan-binding proteins and adhesion P1 (Figure 4), which have high affinity for microorganisms and the tooth surface and thereby promote plaque formation [7,35]. We found that the DD2 culture supernatant reduced the expression levels of both genes, suggesting that DD2 also inhibits the aggregation step of biofilm formation.…”

Section: Discussionmentioning

confidence: 87%

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Antimicrobial and anti-biofilm activities of Lactobacillus kefiranofaciens DD2 against oral pathogens

Jeong

Kim

Song

et al. 2018

Journal of Oral Microbiology

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Background: Streptococcus mutans and Streptococcus sobrinus are major causative bacterial pathogens of dental caries.Objective: We investigated the applicability of three Lactobacillus strains (L. kefiranofaciens DD2, DD5, and DD6) isolated from kefir and three commercial Lactobacillus strains (L. plantarum ATCC 10,012, L. johnsonii JCM 1022, and L. rhamnosus ATCC 7469) as potential oral probiotics with respect to their survivability in an experimental oral environment, antimicrobial activity, and anti-biofilm formation activity against S. mutans and S. sobrinus.Results: Strains DD2, ATCC 10012, ATCC 7469, and JCM 1022 had the best oral survivability, including aerotolerance and enzymatic resistance, and inhibited the growth and biofilm formation of S. mutans and S. sobrinus. In particular, DD2 suppressed all three classes of biofilm formation-associated genes: those associated with carbohydrate metabolism and those encoding regulatory biofilm and adhesion proteins.Conclusions: These results indicate that the novel kefir isolate L. kefiranofaciens DD2 effectively and directly inhibits S. mutans and S. sobrinus.

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Section: Discussionmentioning

confidence: 87%

“…Biofilm formation involves many factors associated with carbohydrate metabolism, adhesion, and regulation [34,35]. The ftf gene encodes fructosyltransferase, which synthesizes fructan polymers that act as binding sites for S. mutans and thereby promote biofilm formation [36] (Figure 4).…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial and anti-biofilm activities of Lactobacillus kefiranofaciens DD2 against oral pathogens

Jeong

Kim

Song

et al. 2018

Journal of Oral Microbiology

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“…Biofilm formation can be considered a developmental process, which is characterized through the identification of structural and regulatory genes required at the various steps of the process (O'Toole et al, 2000;Steinberg, 2000). To gain an insight into similarities and differences in global gene expression between growth in planktonic and biofilm environments, we used S. mutans DNA microarray analysis.…”

Section: Resultsmentioning

confidence: 99%

“…These bacteria are strongly associated with caries formation (Burne et al, 1997;Hamilton, 2000;Kolenbrander, 2000;Liljemark & Bloomquist, 1996;Munson et al, 2004). Following the adhesion of cells to surfaces and accumulation to form multilayered cell clusters, the biofilm bacteria demonstrate a radically different phenotype from the planktonic state (Gilmore et al, 2003;Marsh, 2005, Steinberg, 2000. Sessile bacteria are generally much more tolerant to antibiotics, biocides and hydrodynamic shear forces than their planktonic counterparts (Hall-Stoodley et al, 2004;Stewart & Costerton, 2001).…”

Section: Introductionmentioning

confidence: 99%

Differential gene expression profiling of Streptococcus mutans cultured under biofilm and planktonic conditions

2007

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“…Oral bacteria are the main constituents of the dental biofilm plaque which covers the tooth surface 10,11 and, therefore enamel demineralization is caused by the production of organic acids from bacterial metabolism 12,13 Several factors determine caries development; acid production, plaque adherence onto the enamel surface and bacterial metabolism which is influenced by the dietary regime of the host 14 Caries activity of dental plaque is dependent on the degree of calcium saturation within the plaque which influences demineralization of the enamel surface. 15 The anticariogenic effect of fluoride is well established 16 and widely accepted.…”

Section: Introductionmentioning

confidence: 99%

The in vitro effect of fluoridated milk in a bacterial biofilm - Enamel model

Arnold

Forer²,

Heesen³

et al. 2006

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub

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Objectives:The purpose of this study was to investigate the effect of milk and fluoridated milk on bacterially induced caries-like lesions. Sample and methods: Extracted impacted human molars were cut in half and covered with a varnish leaving a 4×4 mm window. The samples were coated with biofilm of S. sobrinus and were further divided into three experimental groups of S. sobrinus, S. sobrinus and milk and S. sobrinus and fluoridated milk. As negative controls served teeth incubated in saline. Of twenty tooth halves serial ground sections were cut through the lesions and investigated with polarization light microscopy (PLM) and scanning electron microscopy (SEM) and EDX element analysis. The PLM photographs were used for 3D reconstruction, volumetric assessment and determination of the extension of the lesion zones. Of eight tooth halves the biofilm on the enamel surface was studied with SEM and EDX element analysis. Results: Volumetric assessment showed a statistically significant difference in the volume of the body of the lesion and the translucent zone between the milk group and fluoridated milk group. Quantitative element analysis demonstrated significant differences between sound enamel and the superficial layer in the fluoridated milk group. The biofilm on the enamel surface showed an increased Ca content in the milk group and fluoridated milk group. Conclusions: Milk as a common nutrient seems to play a complex role in in-vitro biofilm -enamel interactions stimulating bacterial demineralization on one hand, and, as effective fluoride carrier, inhibits caries-like demineralization.

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Studying Plaque Biofilms on Various Dental Surfaces

Cited by 41 publications

References 0 publications

Antimicrobial and anti-biofilm activities of Lactobacillus kefiranofaciens DD2 against oral pathogens

Antimicrobial and anti-biofilm activities of Lactobacillus kefiranofaciens DD2 against oral pathogens

Differential gene expression profiling of Streptococcus mutans cultured under biofilm and planktonic conditions

The in vitro effect of fluoridated milk in a bacterial biofilm - Enamel model

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