Studying the dynamic mechanism of transporting a single drug carrier-polyamidoamine dendrimer through cell membranes by force tracing

Yang, Boyu; Xu, Hao; Wang, Shaowen; Cai, Mingjun; Shi, Yan; Yang, Guocheng; Wang, Hongda; Shan, Yuping

doi:10.1039/c6nr05838h

Cited by 18 publications

(13 citation statements)

References 27 publications

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“…Since confocal microscopy has a non-invasive ability and can penetrate deep into a sample, it has been applied to many scientific fields, such as drug delivery and virus invasion [26,27]. The principle of confocal microscopy is shown in Figure 2; in confocal microscopy, the illumination and detection are restricted to a diffraction-limited area in the sample, which rejects a higher background and yields a higher resolution in contrast to wide-field epifluorescence microscopy [28,29].…”

Section: Principles Of Atomic Force Microscopy and Optical Microscopymentioning

confidence: 99%

Progress in the Correlative Atomic Force Microscopy and Optical Microscopy

Zhou

Cai

Tong

2017

Sensors

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Atomic force microscopy (AFM) has evolved from the originally morphological imaging technique to a powerful and multifunctional technique for manipulating and detecting the interactions between molecules at nanometer resolution. However, AFM cannot provide the precise information of synchronized molecular groups and has many shortcomings in the aspects of determining the mechanism of the interactions and the elaborate structure due to the limitations of the technology, itself, such as non-specificity and low imaging speed. To overcome the technical limitations, it is necessary to combine AFM with other complementary techniques, such as fluorescence microscopy. The combination of several complementary techniques in one instrument has increasingly become a vital approach to investigate the details of the interactions among molecules and molecular dynamics. In this review, we reported the principles of AFM and optical microscopy, such as confocal microscopy and single-molecule localization microscopy, and focused on the development and use of correlative AFM and optical microscopy.

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Section: Principles Of Atomic Force Microscopy and Optical Microscopymentioning

confidence: 99%

Progress in the Correlative Atomic Force Microscopy and Optical Microscopy

Zhou

Cai

Tong

2017

Sensors

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“…The polyvalent interaction of dendrimers with protein [29,30] resembles a common type of interaction between biological entities such as receptors and ligands or virus and cell surface, etc. [31][32][33][34] Nano-sized carriers systems with dendrimer core are monodisperse therapeutic scaffolds that would possibly allow BDNF delivery to damaged cells, enhancing its local concentration and protein stability against enzymatic degradation. Therefore, we thought to use dendrimers to improve the extracellular retaining of BDNF without the need of covalent chemistry.…”

Section: Introductionmentioning

confidence: 99%

Novel design of (PEG-ylated)PAMAM-based nanoparticles for sustained delivery of BDNF to neurotoxin-injured differentiated neuroblastoma cells

et al. 2020

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Brain-derived neurotrophic factor (BDNF) is essential for the development and function of human neurons, therefore it is a promising target for neurodegenerative disorders treatment. Here, we studied BDNF-based electrostatic complex with dendrimer nanoparticles encapsulated in polyethylene glycol (PEG) in neurotoxin-treated, differentiated neuroblastoma SH-SY5Y cells, a model of neurodegenerative mechanisms. PEG layer was adsorbed at dendrimerprotein core nanoparticles to decrease their cellular uptake and to reduce BDNF-other proteins interactions for a prolonged time. Cytotoxicity and confocal microscopy analysis revealed PEG-ylated BDNF-dendrimer nanoparticles can be used for continuous neurotrophic factor delivery to the neurotoxin-treated cells over 24 h without toxic effect. We offer a reliable electrostatic route for efficient encapsulation and controlled transport of fragile therapeutic proteins without any covalent cross-linker; this could be considered as a safe drug delivery system. Understanding the polyvalent BDNF interactions with dendrimer core nanoparticles offers new possibilities for design of well-ordered protein drug delivery systems. Keywords: Brain-derived neurotrophic factor (BDNF), Poly(amidoamine) dendrimers (PAMAM), Neurotoxin-treated neuroblastoma cells, Model of neurodegenerative mechanisms, Nanoparticles encapsulated in polyethylene glycol (PEG), Controlled transport of fragile therapeutic proteins

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“…27 The polyvalent interaction of dendrimers with protein 28,29 resembles a common type of interaction between biological entities such as receptors and ligands or virus and cell surface, etc. [30][31][32][33] Nano-sized carriers systems with dendrimer core are monodisperse therapeutic scaffolds that would possibly allow BDNF delivery to damaged cells, enhancing its local concentration and protein stability against enzymatic degradation.…”

Section: Introductionmentioning

confidence: 99%

Enhanced brain-derived neurotrophic factor distribution in differentiated human neuroblastoma SH-SY5Y cells by PEG-ylated protein nanoparticles: model of application in neurodegenerative diseases

Dąbkowska

Łuczkowska

Rogińska

et al. 2020

Preprint

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Brain-derived neurotrophic factor (BDNF) is essential for the development and function of human neurons, therefore it is a promising target for neurodegenerative disorders treatment. Here, we studied BDNF-based electrostatic complex with dendrimer nanoparticles encapsulated in polyethylene glycol (PEG) in injured, differentiated neuroblastoma SH-SY5Y cells, a model of neurodegenerative mechanisms. PEG layer was adsorbed at dendrimer-protein core nanoparticles to decrease their cellular uptake and to reduce BDNF-serum proteins interaction for prolonged time. Cytotoxicity and confocal microscopy analysis revealed PEG-ylated BDNF-dendrimer nanoparticles can be used for continuous neurotrophic factor delivery enhancing its distribution into cells over 24 hours without toxic effect. We offer reliable electrostatic route for efficient encapsulation and controlled transport of fragile therapeutic proteins without any covalent cross-linker; this could be considered as safe drug delivery system. Understanding of polyvalent BDNF interactions with dendrimer core nanoparticles offers new possibilities for design of well-ordered protein drug delivery systems.

show abstract

Studying the dynamic mechanism of transporting a single drug carrier-polyamidoamine dendrimer through cell membranes by force tracing

Cited by 18 publications

References 27 publications

Progress in the Correlative Atomic Force Microscopy and Optical Microscopy

Progress in the Correlative Atomic Force Microscopy and Optical Microscopy

Novel design of (PEG-ylated)PAMAM-based nanoparticles for sustained delivery of BDNF to neurotoxin-injured differentiated neuroblastoma cells

Enhanced brain-derived neurotrophic factor distribution in differentiated human neuroblastoma SH-SY5Y cells by PEG-ylated protein nanoparticles: model of application in neurodegenerative diseases

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