Pre-plant treatment of barley seeds with hot mist of biopreparations has some protective effect against harmful microflora, improves the absorption of nutrients from the soil, and increases the physiological activity. For pre-plant treatment of barley seeds of “Creshendo” variety, hot mist of the following biopreparations was used: Baktofit, Potassium humate, Fitosporin M, Innopraktika complex. The evaluation of the influence of the treatment was carried out according to the elements of the structure of the yield of plant samples and the physical-chemical indicators of grain samples from experimental plots. The statistical assessment showed good reliability (coefficient of variation was about 10). The number of productive stems per 1 m2 was increased by the hot mist treatment with Innopraktika complex by 54.1 % and Fitosporin M by 31.0 % in comparison with the samples from the control plot. The weight of 1,000 grains exceeded by 5.9 % in samples treated with Potassium humate and by 4.6 % with Fitosporin M. The highest biological yield was achieved when hot mist treatment with Innopraktika complex (50.822 dt/ha) and Fitosporin M (43.17 dt/ha). In terms of physical-chemical indicators, the best values were obtained when treated with Fitosporin M and Potassium humate in comparison with control samples: an increase in the mass fraction of starch in grain was by 10.8 % and 5.1 %, respectively; crude protein increased by 5.4 % and 6.9 %; an increase in the exchange energy in both cases was by 0.8 %. The best effect of pre-plant treatment of barley seeds of “Creshendo” variety is achieved with the use of hot mist of biopreparations Fitosporin M and Innopraktika complex.