Studying the interaction of carbohydrate–protein on the dendrimer-modified solid support by microarray-based plasmon resonance light scattering assay

Development of Sphere-Polymer Brush Hierarchical Nanostructure Substrates for Fabricating Microarrays with High Performance

“…For biotin-Con A, the fluorescence intensity of Man-α spots is stronger than that of Glc-β spots, whereas the fluorescence intensity of Gal-β spots is relatively poor. These results are consistent with the previous literature reports, which suggest that the PGMA@3D(160) substrate-based carbohydrate microarray has good selectivity and can be used to discriminate the binding affinities of lectins with saccharides. , …”

“…These results are consistent with the previous literature reports, which suggest that the PGMA@3D(160) substrate-based carbohydrate microarray has good selectivity and can be used to discriminate the binding affinities of lectins with saccharides. 32,41 A series of experiments were designed to evaluate the sensitivity of PGMA@3D(160) substrate-based carbohydrate microarrays. Amino-modified monosaccharides (Gal-β and Man-α) with various concentrations in printing solution 1 were first immobilized on PGMA@3D(160) substrates and then incubated in the corresponding lectin solution.…”

Multiplex profiling of glycoproteins using a novel bead‐based lectin array

“…Bio-Plex suspension array achieves biological affinity reactions on the microspheres in 3D format, and the fluorescent intensities of the microspheres are subsequently identified and detected individually, providing high sensitivity and specificity for immunoassay [14]. Previous report also proved that binding affinity between lectins and carbohydrates can be increased in 3D condition [23]. The present study took advantages of Bio-Plex system to conjugate lectins onto the microspheres optimally, thus, achieving higher sensitivity for glycosylation assay.…”

Carbohydrate microarrays fabricated on poly(2-methylacrylic acid)-based substrates for analysis of carbohydrate–protein interactions

“…In order to improve the performance of carbohydrate microarray-based assays, more recent studies have focused on improving the detection sensitivity by increasing specific binding and decreasing background noise. 9 On one hand, increasing the carbohydrate loading capability 10 and varying the carbohydrate presentation on the substrates, such as the spacing and orientation of the carbohydrate ligands to guarantee multivalent binding, can significantly enhance specific binding. Liu et al adopted silica nanoparticle-coated glass substrates with post-modification with a poly(glycidylmethacrylate) brush layer for carbohydrate microarray fabrication so as to improve the carbohydrate loading capacity on the substrates, and the obtained carbohydrate microarrays exhibited good sensitivity and strong multivalent interactions.…”