Studying the Variations of Complex Electrical Bio-Impedance of Plant Tissues During Boiling

Bera, Tushar Kanti; Bera, Sampa; Kar, Kalyan; Mondal, Shubha

doi:10.1016/j.protcy.2016.03.024

Cited by 12 publications

(10 citation statements)

References 54 publications

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“…As R r >> R w (e.g., Ehosioke et al., 2018 showed that R r ≈ 1 MΩ), the resistor with the low resistivity (the nutrient solution) controls the total resistance of the system. Since the current does not pass through the cell membrane, the major volume of the root can be considered to be highly resistive (Bera, Bera, Kar, & Mondal, 2016). The main outcome of this is that as long as the connection between roots and the growing media can be considered in parallel (i.e., current is not forced to cross through the root), and R r ≫ R w , the contribution of roots to the in‐phase conductivity is expected to be small.…”

Section: Resultsmentioning

confidence: 99%

Relationship between wheat root properties and its electrical signature using the spectral induced polarization method

Tsukanov

Schwartz

2020

Vadose Zone Journal

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Measuring root properties, the "hidden half" of the plant, is challenging due to their heterogeneous and dynamic nature. A promising method for noninvasive mapping of roots and their activity, spectral induced polarization (SIP), has been introduced. However, measurements of root properties together with their SIP responses are missing, limiting the interpretation of a root's SIP signature. In this study, we coupled SIP measurements of roots in hydroponic solution with measurements of root biomass, surface area, and diameter. Furthermore, we monitored the SIP response of roots poisoned by cyanide, which results in depolarization of the root's cell membrane potential. We found a linear correlation between root biomass and surface area, and the low-frequency electrical polarization. In addition, we demonstrate the relationship between root cell membrane potential and root polarization. Based on the results, we suggest that in comparison with the stem-based approach used by other researchers, the polarization in the contact-free method used in this study is related to the external surface area of the root and external architectural structures such as root diameter and root hair. Overall, a direct link between root properties and their electrical signature was established. 1 INTRODUCTION Roots are the "hidden half" of plants (Eshel & Beeckman, 2013), and despite ever-growing efforts to study their structure, dynamics, and functions, our understanding of roots is still incomplete, especially in comparison with the aboveground part of the plant (Ryan, Delhaize, Watt, & Richardson, 2016). The lag in root research is mainly due to root heterogeneity, their dynamic nature, opacity of the growing media, and the absence of a technology that will overcome these

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Section: Resultsmentioning

confidence: 99%

Relationship between wheat root properties and its electrical signature using the spectral induced polarization method

Tsukanov

Schwartz

2020

Vadose Zone Journal

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“…Electrical impedance spectroscopy determines the impedance of the soil–plant continuum across a range of frequencies (hertz to megahertz). This method has been used in many studies in plant science and medicine (Bera, Bera, et al., 2016; Bera, Nagaraju, & Lubineau, 2016; Coster, Chilcott, & Coster, 1996; Hayden, Moyse, Calder, Crawford, & Fensom, 1969; Inaba, Manabe, Tsuji, & Lwamuto, 1995; Klauke et al., 2005; Lin, Chen, & Chen, 2012; Macdonald, 1992; Shrivastava, Barde, Mishra, & Phadke, 2014a, 2014b). For root studies, EIS has been used to assess the morphological and physiological properties of roots such as root growth (Ozier‐Lafontaine & Bajazet, 2005; Repo, Laukkanen, & Silvennoinen, 2005), estimation of root system size (Cao, Repo, Silvennoinen, Lehto, & Pelkonen, 2011), and mycorrhizal colonization of roots (Cseresnyés, Takács, Végh, Anton, & Rajkai, 2013; Repo, Korhonen, Laukkanen, Lehto, & Silvennoinen, 2014; Repo, Korhonen, Lehto, & Silvennoinen, 2016), as summarized in Table 4.…”

Section: Methods Targeting Electrical Resistivitymentioning

confidence: 99%

“…Instead, the current passes through the apoplast (Bera, Bera, Kar, & Mondal, 2016; Repo et al., 2012), as shown in Figure 1b. In this low‐frequency case, the total impedance will be mainly determined by the resistance of the extracellular fluid (Bera, Bera, et al., 2016; Repo et al., 2012); this is termed “counterion polarization” (Table 1). As the frequency increases, the applied electrical field at the outer surface of the membrane changes the transmembrane potential difference that regulates the gating of ion channels and ion fluxes across the cell membranes (Hille, 2001; Kinraide, 2001; Mathie et al., 2003).…”

Section: Introductionmentioning

confidence: 99%

“…The opening of ion channels leads to decreased negativity of the membrane surface potential and an increased cation flux into the cell that results in a weaker EDL (Kessouri et al., 2019; Kinraide, 2001; Kinraide & Wang, 2010; Wang, Kinraide, Zhou, Kopittke, & Peijnenburg, 2011; Weigand & Kemna, 2019), and a lower polarizability of the EDL. At these high frequencies, current thus flows through the entire cell and crosses different interfaces (Figure 1c) such that the resulting total impedance will be a combination of the properties of the apoplast and the extracellular fluid, the cell membranes, the cytoplasm, and the ICF (Bera, Bera, et al., 2016; Repo et al., 2012). In this case, the polarization is considered to be interfacial (Table 1).…”

Section: Introductionmentioning

confidence: 99%

“…In this case, the EDL prevents any passage of current through the cell membrane (i.e., the impedance of the cell membranes is so high that it does not allow current passage). Instead, the current passes through the apoplast (Bera, Bera, Kar, & Mondal, 2016; Repo et al., 2012), as shown in Figure 1b. In this low‐frequency case, the total impedance will be mainly determined by the resistance of the extracellular fluid (Bera, Bera, et al., 2016; Repo et al., 2012); this is termed “counterion polarization” (Table 1).…”

Section: Introductionmentioning

confidence: 99%

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Sensing the electrical properties of roots: A review

Ehosioke

Nguyen

Rao

et al. 2020

Vadose Zone Journal

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Thorough knowledge of root system functioning is essential to understand the feedback loops between plants, soil, and climate. In situ characterization of root systems is challenging due to the inaccessibility of roots and the complexity of root zone processes. Electrical methods have been proposed to overcome these difficulties. Electrical conduction and polarization occur in and around roots, but the mechanisms are not yet fully understood. We review the potential and limitations of low‐frequency electrical techniques for root zone investigation, discuss the mechanisms behind electrical conduction and polarization in the soil–root continuum, and address knowledge gaps. A range of electrical methods for root investigation is available. Reported methods using current injection in the plant stem to assess the extension of the root system lack robustness. Multi‐electrode measurements are increasingly used to quantify root zone processes through soil moisture changes. They often neglect the influence of root biomass on the electrical signal, probably because it is yet to be well understood. Recent research highlights the potential of frequency‐dependent impedance measurements. These methods target both surface and volumetric properties by activating and quantifying polarization mechanisms occurring at the root segment and cell scale at specific frequencies. The spectroscopic approach opens up a range of applications. Nevertheless, understanding electrical signatures at the field scale requires significant understanding of small‐scale polarization and conduction mechanisms. Improved mechanistic soil–root electrical models, validated with small‐scale electrical measurements on root systems, are necessary to make further progress in ramping up the precision and accuracy of multi‐electrode tomographic techniques for root zone investigation.

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Electrical bioimpedance spectroscopy as a non-invasive monitoring tool of physiological states of macroalgae tissues: example on the impact of electroporation on 8 different seaweed species

Robin,

Levkov,

González-Díaz

et al. 2024

Eur Food Res Technol

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In this study, we evaluated Electrical Impedance Spectroscopy (EIS) as a monitoring tool of the physiological state of Bryopsis, Cystoseira, Stypopodium, Cladophora, Taonia, Padina, Ulva and Sargassum tissues. We analyzed the electrical response differences in the EIS between species and in the same seaweed tissue before and after electroporation. Electroporation using high voltage pulsed electric field (PEF) treatment was used as a model for cell disruption affecting the tissue physiology without being noticeable to the naked-eye. Significant differences in all the seaweeds were observed before and after electroporation. We found that seaweed species with smaller and rounder cells have a clearer dispersion profile (around a frequency of 10–100 kHz) compared to the dispersion profile of seaweed with larger cells with unround form. Those results suggest that EIS could be used as a fast non-invasive monitoring technique of the changes in the physiology of seaweeds.

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Studying the Variations of Complex Electrical Bio-Impedance of Plant Tissues During Boiling

Cited by 12 publications

References 54 publications

Relationship between wheat root properties and its electrical signature using the spectral induced polarization method

Relationship between wheat root properties and its electrical signature using the spectral induced polarization method

Sensing the electrical properties of roots: A review

Electrical bioimpedance spectroscopy as a non-invasive monitoring tool of physiological states of macroalgae tissues: example on the impact of electroporation on 8 different seaweed species

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