STXBP5L and its related molecules as potential biomarkers in lymph node metastatic breast cancer

Abstract

BackgroundLymph node metastasis is a common problem in breast cancer, and its underlying molecular mechanism is still unclear. The purpose of this study is to research the molecular mechanism and to explore the key RNAs and pathways that mediate lymph node metastasis in breast cancer.MethodsGSE100453 and GSE38167 were downloaded from the Gene Expression Omnibus (GEO) database and 569 breast cancer statistics were also downloaded from the TCGA database. Differentially expressed miRNAs were calculated by using R software and GEO2R. Gene ontology and Enriched pathway analysis of target mRNAs were analyzed by using DAVID and R software. The PPI network was constructed by using Metascape and Cytoscape software.ResultsIn total, 6 differentially expressed miRNAs were selected, and 499 mRNAs were identified after filtering. The research of KEGG indicated that mRNAs enriched in many cancer pathways. Besides, some hub mRNAs were analyzed after constructing the PPI network. A total of 3 out of 6 miRNAs had a significant relationship with the overall survival (P<0.05) and showed a good ability of risk prediction model of over survival.ConclusionBy utilizing bioinformatics analyses, differently expressed miRNAs were identified and constructed a complete gene network. Several potential mechanisms and therapeutic and prognostic targets of lymph node metastasis were also demonstrated in breast cancer.