Sub-chronic toxicity of Garcinia atroviridis Griff Fruit's ethanol extract on Wistar rats (Ratus norvegicus)

Abstract

Garcinia atroviridis Griff. (GA) is a tropical fruit that commonly used as a traditional medicine to this day. This study was conducted to determine the sub-chronic toxicity effect of GA fruits ethanol extract on body weight, clinical hematology and biochemical parameters, and organ's histopathology. This study is an experimental research by oral treatments for 90 days with completely randomized design. The treatment group consists of five classes. Each class was given the treatment with a dosage of 50 mg/kg BW, 200 mg/kg BW, 800 mg/kg BW and satellite group with 2% PGA, or 800 mg/kgBW. Based on ANOVA and advanced Tukey test results using SPSS, the hematological parameters such as mean corpuscular volume, mean corpuscular hemoglobin concentration, and white blood cell had significant differences with the control group. In the biochemical parameters, the serum glutamic-oxaloacetic transaminase values and triglycerides (in male rats), serum glutamic-pyruvic transaminase and creatinine (in female rats) had significant differences with the control group. In conclusion, GA fruits ethanol extract is safe and non toxic to body weight, clinical hematology and biochemical parameters, and histopathology of ten organs.

Kajian Toksisitas Tanaman Genus Garcinia

“…Secara analisis, berat badan menurun ditandai dengan penurunan konsumsi makanan dan air yang signifikan (p<0,05), tidak ada perbedaan yang signifikan antara kelompok kontrol dan kelompok pengujian (p≥0,05) pada parameter biokimia darah, hematologi, dan berat organ relatif limpa meningkat pada dosis 5000 mg/KgBB , serta nilai LD50 > 5000 mg/KgBB (Dyary et al, 2016) Ekstrak etanol buah Garcinia atroviridis dilakukan pengujian toksisitas subkronis dengan pemberian dosis oral sebesar 50, 200 dan 800 mg/KgBB tidak menunjukkan adanya gejala klinis dan tidak bersifat toksik berdasarkan profil parameter uji toksisitas subkronis. Ekstrak etanol buah Garcinia atroviridis dengan nilai LD50 2000 mg/KgBB tidak menyebabkan gejala toksisitas akut pada tikus (Tiana et al, 2019).…”