Sub-lethal Camphor Exposure Triggers Oxidative Stress, Cardiotoxicity, and Cardiac Physiology Alterations in Zebrafish Embryos

Du, Zhenhong; Xia, Zhong-Shang; Zhang, Mingzhe; Wei, Yanting; Malhotra, Nemi; Saputra, Ferry; Audira, Gilbert; Roldan, Marri Jmelou M.; Hsiao, Chung-Der; Hao, Erhong; Hou, Xin; Deng, Jiagang

doi:10.1007/s12012-021-09682-x

Cited by 7 publications

(2 citation statements)

References 46 publications

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“…Furthermore, we hypothesized that our program was capable of detecting bradycardia (heartbeat slow down) and cardiac dysregulation (arrhythmia). In order to validate our hypothesis, we used a previously published dataset showing heart rate slow down and cardiac rhythm dysregulation after 500 ppm Camphor exposure reported by Du et al [76]. After 500 ppm Camphor exposure, we found a significant slowdown in the heart rate in both atrium and ventricle chambers compared to control fish (Figure 6A,B).…”

Section: Use Of Opencv Methods To Detect Cardiac Rhythm Dysregulation After Camphor Treatmentsupporting

confidence: 59%

An OpenCV-Based Approach for Automated Cardiac Rhythm Measurement in Zebrafish from Video Datasets

et al. 2021

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Cardiac arrhythmia has been defined as one of the abnormal heart rhythm symptoms, which is a common problem dealt with by cardiologists. Zebrafish were established as a powerful animal model with a transparent body that enables optical observation to analyze cardiac morphology and cardiac rhythm regularity. Currently, research has observed heart-related parameters in zebrafish, which used different approaches, such as starting from the use of fluorescent transgenic zebrafish, different software, and different observation methods. In this study, we developed an innovative approach by using the OpenCV library to measure zebrafish larvae heart rate and rhythm. The program is designed in Python, with the feature of multiprocessing for simultaneous region-of-interest (ROI) detection, covering both the atrium and ventricle regions in the video, and was designed to be simple and user-friendly, having utility even for users who are unfamiliar with Python. Results were validated with our previously published method using ImageJ, which observes pixel changes. In summary, the results showed good consistency in heart rate-related parameters. In addition, the established method in this study also can be widely applied to other invertebrates (like Daphnia) for cardiac rhythm measurement.

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Section: Use Of Opencv Methods To Detect Cardiac Rhythm Dysregulation After Camphor Treatmentsupporting

confidence: 59%

An OpenCV-Based Approach for Automated Cardiac Rhythm Measurement in Zebrafish from Video Datasets

et al. 2021

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“…In fact, secondary photo-oxidation of the terpenes can determine the generation of unstable allylic hydroperoxides whose reduction and subsequent oxidation can lead to the formation of alcohols and then aldehydes or ketones [24]. These compounds are studied for their potential toxicity, inherent to the promotion of oxidative stress [25,26].…”

Section: Introductionmentioning

confidence: 99%

An Optimized Terpene Profile for a New Medical Cannabis Oil

et al. 2022

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The purpose of this analytical study was to develop an advanced formulation of medical Cannabis oil (MCO) comparing the chemical profile of different extracts obtained with two existing methods (SIFAP and CALVI) and one original upgraded (CERFIT) method. Preparation methods were applied with varying solvent, temperature, and duration of the decarboxylation and extraction steps. HPLC-MS/MS TSQ and GC/FID-HS analyses were performed to investigate cannabinoid and terpene contents in the three oil extracts. Cannabinoids profile remained comparable between the formulations. CERFIT extracts exhibited a superior quantity of total terpene hydrocarbon forms (e.g., limonene and α-pinene) with no degradation occurrence (i.e., oxidized terpenes not quantifiable). Thus, this new method optimized the phytochemical profile of the MCO presenting a value opportunity to obtain a standardized high-level therapeutic product.

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Exposure to Pyriproxyfen Impacts Heart Development Causing Tissue and Cellular Impairments, Heart Arrhythmia and Reduced Embryonic Growth

Bernhardt,

Ronconi-Krüger,

Nazari

2024

Cardiovasc Toxicol

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Sub-lethal Camphor Exposure Triggers Oxidative Stress, Cardiotoxicity, and Cardiac Physiology Alterations in Zebrafish Embryos

Cited by 7 publications

References 46 publications

An OpenCV-Based Approach for Automated Cardiac Rhythm Measurement in Zebrafish from Video Datasets

An OpenCV-Based Approach for Automated Cardiac Rhythm Measurement in Zebrafish from Video Datasets

An Optimized Terpene Profile for a New Medical Cannabis Oil

Exposure to Pyriproxyfen Impacts Heart Development Causing Tissue and Cellular Impairments, Heart Arrhythmia and Reduced Embryonic Growth

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