Sub-optimal or reduction in temperature and salinity decrease antioxidant activity and cellularity in the hemolymph of the Pacific abalone (Haliotis discus hannai)

Yang, Sung Jin; Min, Byung Hwa

doi:10.1016/j.fsi.2018.10.041

Cited by 15 publications

(10 citation statements)

References 35 publications

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“…Considering GSH as the substrate of GPX, it neutralizes hydrogen peroxide produced by SOD and has the effects of antioxidant and integrated detoxification, some studies have suggested that it plays an important role in osmotic and oxidative stress (Figueira et al, 2005;Manduzio et al, 2005;Paital and Chainy, 2010). In this study, the GSH of black P. fucata decreased significantly with the increase of salinity at 1.5 and 3 h. The result was consistent with the findings reported in three Veneridae clams (Venerupis decussata, Venerupis corrugate, and Venerupis philippinarum) (Carregosa et al, 2014), Pacific abalone (H. discus hannai) (Yang and Min, 2019), and arcid blood clam (Anadara granosa) (Anthony and Patel, 2000). Our results may suggest that black P. fucata can enhance the antioxidant defense ability to cope with the low salinity environment, while the antioxidant defense ability was inhibited under high salinity conditions.…”

Section: Fitness Performancesupporting

confidence: 91%

Physical Responses of Pinctada fucata to Salinity Stress

Yang

Chen

et al. 2022

Front. Mar. Sci.

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This study was conducted to understand the changes of physiological and biochemical indexes of black and red shell Pinctada fucata under acute high and low salt stress. In this study, the salinity of 35‰ was used as the control, while the salinities of 20 and 50% salinity were used as the low and high salt treatment groups, respectively. The osmotic pressure (OSM) and ion concentration in the hemolymph, Na+-K+ -ATPase (NKA) activity and respiratory metabolism in gills, and antioxidant and immune (non) enzymes in the hepatopancreas of P. fucata with two shell colors were compared and analyzed at the time periods of 1.5 and 3 h post-salinity stress. The results showed that the OSM and inorganic ion (Na+, Ca2+, and Cl–) concentration in the hemolymph of the black and red P. fucata increased significantly with the increase of salinity after the time periods of 1.5 and 3 h. At 3 h, the black P. fucata NKA activity decreased significantly with the increase of salinity, while red P. fucata reached the highest value at high salinity. The succinate dehydrogenase (SDH) and lactate dehydrogenase (LDH) activities of red P. fucata showed U-shaped and inverted U-shaped distributions with the increase of salinity after 1.5 h, respectively. With the increase of salinity, the phenoloxidase (POX) activity of red and black P. fucata showed inverted U-shaped and U-shaped distributions, respectively. The contents of glutathione (GSH) and vitamin C (VC) in black P. fucata decreased significantly with the increase of salinity at 1.5 and 3 h. Red P. fucata GSH and VC reached their maximum value in the 1.5-h low salinity group and 3-h high salinity group. The vitamin E (VE) content in black P. fucata increased significantly with the increase of salinity at 1.5 h, and reached the maximum at 3 h in the control group. Red P. fucata VE reached the maximum at 1.5 and 3 h in the control group. The results obtained from the present study revealed that the sensitivity of P. fucata to salinity varied in shell color. Compared to black P. fucata, red P. fucata responds more quickly to sharp salinity changes, thereby reducing more likely damage. Compared with a high salt environment, P. fucata was more adaptable to the changes of acute low salt environment. The results obtained from the present study provide the physical references for subsequent selective breeding of this species.

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Section: Fitness Performancesupporting

confidence: 91%

Physical Responses of Pinctada fucata to Salinity Stress

Yang

Chen

et al. 2022

Front. Mar. Sci.

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“…In the absence of mechanisms to reduce the thermal sensitivity of their metabolism 11 or to compensate for the increased cost of metabolism 2 during the summer, organisms will experience a metabolic deficit that may suppress maintenance and further growth. It is generally accepted that gastropods become quiescent as environmental temperatures decrease (below 7–8 °C in the case of H. discus hannai ) 38,39 , allowing them to reduce their metabolic rate. Indeed, higher Q 10 values indicate that the metabolic rates of abalone are more rapidly reduced at lower temperatures than would be predicted from their response to higher temperatures.…”

Section: Discussionmentioning

confidence: 99%

Physiological responses of the abalone Haliotis discus hannai to daily and seasonal temperature variations

Kang

Lee

Song

et al. 2019

Sci Rep

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Organisms inhabiting tidal mixing-front zones in shallow temperate seas are subjected to large semidiurnal temperature fluctuations in summer. The ability to optimize energy acquisition to this episodic thermal oscillation may determine the survival, growth and development of these ectotherms. We compared the physiological and molecular responses of Haliotis discus hannai cultivated in suspended cages to fluctuating or stable temperature conditions. Several physiological indicators (respiration, excretion rates and O:N) were measured in both conditions, and alterations in the proteome during thermal fluctuations were assessed. No summer mortality was observed in abalone cultivated in fluctuating temperatures compared with that at stable high temperatures. Metabolic rates increased sharply during stable warm summer conditions and fluctuated in accordance with short-term temperature fluctuations (20–26 °C). Ammonia excretion rates during acute responses were comparable in both conditions. When abalone were exposed to fluctuating temperatures, enzyme activities were downregulated and structure-related protein expression was upregulated compared with that at an acclimation temperature (26 °C), highlighting that exposure to low temperatures during fluctuations alters molecular processes. Our results reveal that modulation of physiological traits and protein expression during semidiurnal thermal fluctuations may buffer abalone from the lethal consequences of extreme temperatures in summer.

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“…Moreover, abalone encounters a sudden drop in seawater salinity during severe summer rainstorms and typhoon events [ 5 ]. However, changes in ambient salinity could significantly affect abalone inflammatory and immune responses and are associated with several other physiological responses, such as disrupting electrolyte equilibrium, oxidative damage, and metabolism disorders [ 6 – 9 ], which eventually influence their survival and growth [ 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome analysis reveals fluid shear stress (FSS) and atherosclerosis pathway as a candidate molecular mechanism of short-term low salinity stress tolerance in abalone

et al. 2022

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Background Transcriptome sequencing is an effective tool to reveal the essential genes and pathways underlying countless biotic and abiotic stress adaptation mechanisms. Although severely challenged by diverse environmental conditions, the Pacific abalone Haliotis discus hannai remains a high-value aquaculture mollusk and a Chinese predominantly cultured abalone species. Salinity is one of such environmental factors whose fluctuation could significantly affect the abalone’s cellular and molecular immune responses and result in high mortality and reduced growth rate during prolonged exposure. Meanwhile, hybrids have shown superiority in tolerating diverse environmental stresses over their purebred counterparts and have gained admiration in the Chinese abalone aquaculture industry. The objective of this study was to investigate the molecular and cellular mechanisms of low salinity adaptation in abalone. Therefore, this study used transcriptome analysis of the gill tissues and flow cytometric analysis of hemolymph of H. discus hannai (DD) and interspecific hybrid H. discus hannai ♀ x H. fulgens ♂ (DF) during low salinity exposure. Also, the survival and growth rate of the species under various salinities were assessed. Results The transcriptome data revealed that the differentially expressed genes (DEGs) were significantly enriched on the fluid shear stress and atherosclerosis (FSS) pathway. Meanwhile, the expression profiles of some essential genes involved in this pathway suggest that abalone significantly up-regulated calmodulin-4 (CaM-4) and heat-shock protein90 (HSP90), and significantly down-regulated tumor necrosis factor (TNF), bone morphogenetic protein-4 (BMP-4), and nuclear factor kappa B (NF-kB). Also, the hybrid DF showed significantly higher and sustained expression of CaM and HSP90, significantly higher phagocytosis, significantly lower hemocyte mortality, and significantly higher survival at low salinity, suggesting a more active molecular and hemocyte-mediated immune response and a more efficient capacity to tolerate low salinity than DD. Conclusions Our study argues that the abalone CaM gene might be necessary to maintain ion equilibrium while HSP90 can offset the adverse changes caused by low salinity, thereby preventing damage to gill epithelial cells (ECs). The data reveal a potential molecular mechanism by which abalone responds to low salinity and confirms that hybridization could be a method for breeding more stress-resilient aquatic species.

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Sub-optimal or reduction in temperature and salinity decrease antioxidant activity and cellularity in the hemolymph of the Pacific abalone (Haliotis discus hannai)

Cited by 15 publications

References 35 publications

Physical Responses of Pinctada fucata to Salinity Stress

Physical Responses of Pinctada fucata to Salinity Stress

Physiological responses of the abalone Haliotis discus hannai to daily and seasonal temperature variations

Transcriptome analysis reveals fluid shear stress (FSS) and atherosclerosis pathway as a candidate molecular mechanism of short-term low salinity stress tolerance in abalone

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