Sub-picomole high-performance liquid chromatographic/mass spectrometric determination of glutathione in the maize (Zea mays L.) kernels exposed to cadmium

Klejdus, Bořivoj; Zehnálek, Josef; Adam, Vojtěch; Petřek, Jiřı́; Kizek, René; Vacek, Jan; Trnková, Libuše; Rozik, Roland; Havel, Ladislav; Kubáň, Vlastimil

doi:10.1016/j.aca.2004.02.060

Cited by 52 publications

(29 citation statements)

References 33 publications

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“…Oxidative stress is considered to be involved in ageing (14)(15)(16)(17)(18)(19)(20) Redox status expressed as GSH:GSSG ratio as a marker for oxidative stress in paediatric tumour patients (36)(37)(38)(39)(40)(41)(42)(43)(44). There is an increasingly growing interest in identifying biomarkers for diseases, in which oxidative stress is involved (45). For many years, GSH has been measured by several analytical methods.…”

Section: Introductionmentioning

confidence: 99%

Redox status expressed as GSH:GSSG ratio as a marker for oxidative stress in paediatric tumour patients

et al. 2012

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Abstract.Oxidative stress causes profound alterations of various biological structures, including cellular membranes, lipids, proteins and nucleic acids, and it is involved in numerous malignancies. Reduced glutathione (GSH) is considered to be one of the most important scavengers of reactive oxygen species (ROS), and its ratio with oxidised glutathione (GSSG) may be used as a marker of oxidative stress. The main aim of this study was to determine GSH:GSSG ratio in the blood serum of paediatric cancer patients to use this ratio as a potential marker of oxidative stress. The whole procedure was optimised and the recoveries for both substances were greater than 80% under the optimised conditions. We analysed a group of paediatric patients (n=116) with various types of cancer, including neuroblastoma, anaplastic ependymoma, germ cell tumour, genital tract tumour, lymphadenopathy, rhabdomyosarcoma, nephroblastoma, Ewing's sarcoma, osteosarcoma, Hodgkin's lymphoma, medulloblastoma and retinoblastoma. We simultaneously determined the levels of reduced and oxidised glutathione, and thus, its ratio in the blood serum of the patients. The highest ratio was observed in retinoblastoma patients and the lowest in anaplastic ependymoma. We were able to distinguish between the diagnoses based on the results of the obtained GSH:GSSG ratio.

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Section: Introductionmentioning

confidence: 99%

Redox status expressed as GSH:GSSG ratio as a marker for oxidative stress in paediatric tumour patients

et al. 2012

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“…PCs have a basic formula (γ-Glu-Cys)n-Gly (n = 2 to 11) and form M-PC complexes with the heavy metals (M), in which the metal is bound via the cysteine unit SH group [1,6,7]. A very wide range of analytical techniques, including both chromatographic coupled with different detectors and stationary ones, such as electrochemical methods, have been utilized for determination of thiols in samples of interest [8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24].…”

Section: Introductionmentioning

confidence: 99%

Multi-instrumental Investigation of Affecting of Early Somatic Embryos of Spruce by Cadmium(II) and Lead(II) Ions

Šupálková

Petřek

Baloun

et al. 2007

Sensors

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Abstract:The main aim of this work was to use multi-instrumental analytical apparatus to investigate the effects of treatment with cadmium(II) and/or lead(II) ions (50, 250 and 500 µM) for twelve days on early somatic spruce embryos (ESEs). Primarily we used image analysis for estimation of growth and a fluorimetric sensor for enzymatic detection of viability of the treated ESEs. It follows from the obtained results that Cd caused higher toxicity to ESEs than Pb. Besides this fundamental finding, we observed that ESEs grew and developed better in the presence of 500 µM of the metal ions than in the presence of 250 µM. Based on the results obtained using nuclear magnetic resonance this phenomenon was related to an increase of the area of ESE clusters by intensive uptake of water from the cultivation medium, due to dilution of the heavy metal concentration inside the cluster. In addition we studied the glutathione content in treated ESEs by the adsorptive transfer stripping technique coupled with the differential pulse voltammetry Brdicka reaction. GSH contents increased up to 148 ng/mg (clone 2/32) and 158 ng/mg (clone PE 14) after twelve Sensors 2007, 7 744 day long treatment with Cd-EDTA ions. The GSH content was about 150 and 160 % higher in comparison with the ESEs treated with Pb-EDTA ions, respectively. The difference between GSH contents determined in ESEs treated with Pb-EDTA and Cd-EDTA ions correlates with the higher toxicity of cadmium(II) ions.

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“…The accumulation of metals in various plant parts of Zea mays L. has been reported in a number of studies (Jarausch -Wehrheim et al, 1996; Xu et al, 2002;Klejdus et al, 2004). Heavy metal accumulation in edible plant parts may adversely affect consumers health.…”

Section: Soils Adjacent To Major Roadsmentioning

confidence: 99%

Medical geochemical investigations in taking precautionary measures against diseases. Protection and human health

2013

Global NEST Journal

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A considerable number of diseases are directly related to environmental impact. Toxic metals such as Hg, Pb, Cd, and As may damage significantly the human health when they exceed certain levels in the body. For example specific precautions should be taken for the diet of pregnant women and the children. Lead concentrations exceeding the safe values can cause severe damage to the development of central nervous system, as well as a general developmental delay of fetuses and young children, interfering with the functioning of almost every brain neurotransmitter. In particular for the pregnant women, it has been found that the exposure of the fetus on high lead values may cause, apart from neurological and behavioral problems, low birth weight, pre-term delivery, spontaneous abortion and stillbirth. Organic mercury (methyl mercury) is the most dangerous form of mercury, because it is the most easily absorbed orally and crosses into the brain and fetus so readily. Populations exposed to chemical compounds containing As, Ni, Cr, Cd, etc. are considered of high-risk in developing cancer.Environmental geochemical studies can help in assessing the quality of the environment as well as the determination of the sources of pollutants, their behaviour and other characteristics. This knowledge is necessary in any application of remediation methodologies and waste management for the prevention of pollutants in getting into the food chain. It is also used in determining safe criteria regarding the quality of soils, drinking water, construction of schools, playgrounds etc. In this work the importance of environmental geochemical research and its applications towards the protection of human health is demonstrated. KEYWORDS:Medical geochemistry, metals in human health, metals in cancer disease, metals in nervous diseases, Pb in fetus development, remediation methods, and job diseases.

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Sub-picomole high-performance liquid chromatographic/mass spectrometric determination of glutathione in the maize (Zea mays L.) kernels exposed to cadmium

Cited by 52 publications

References 33 publications

Redox status expressed as GSH:GSSG ratio as a marker for oxidative stress in paediatric tumour patients

Redox status expressed as GSH:GSSG ratio as a marker for oxidative stress in paediatric tumour patients

Multi-instrumental Investigation of Affecting of Early Somatic Embryos of Spruce by Cadmium(II) and Lead(II) Ions

Medical geochemical investigations in taking precautionary measures against diseases. Protection and human health

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