Subcellular Localization of Rat Brain Esterases

Bernsohn, Joseph; Barron, Kevin D.; Doolin, Paul F.; Hess, Adeline R.; Hedrick, Marjorie T.

doi:10.1177/14.6.455

Cited by 52 publications

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“…Short and long chain naphthyl ester substrates are hydrolysed by esterase enzymes associated with the brain myelin samples used in this work, in agreement with previous observations with myelin from other animal species (BERNSOHN et al, 1966;FREY et al, 1971). A marked specificity for the shorter chain length substrates is demonstrated in the present study.…”

Section: Discussionsupporting

confidence: 93%

“…The present results demonstrate high esterase activity in the microsomal fraction of brain tissue confirming earlier observations by BERNSOHN et al (1966). In view of these findings, myelin samples were assayed further for the presence of possible microsomal contamination which could affect the esterase measurements even though our previous studies (RUMSBY et al, 1970) suggested negligible activity from other membrane structures.…”

Section: Discussionsupporting

confidence: 91%

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On the Association of Non‐specific Esterase Activity With Central Nerve Myelin Preparations

Rumsby

Getliffe

Riekkinen

1973

Journal of Neurochemistry

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Abstract— Isolated bovine central nerve myelin sheath preparations showed non‐specific esterase activity towards naphthyl ester substrates of increasing chain length from acetate to palmitate. Short chain esters were hydrolysed much faster than long chain substrates by myelin, the specific activity for the hydrolysis of β‐naphthyl acetate being the highest. Micro‐somal fractions from brain white matter were much higher in esterase activity to all naphthyl ester substrates. NADPH‐cytochrome c reductase activity was absent from isolated myelin samples. Distilled water and salt and buffer solutions of different ionic strengths and pH were ineffective in releasing non‐specific esterase activity from myelin although tri‐potassium citrate caused marked inhibition of the membrane‐bound esterase activity. The detergent Triton X‐100 released esterase activity from the myelin preparations but at a concentration of 0.1 per cent was also inhibitory.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 91%

On the Association of Non‐specific Esterase Activity With Central Nerve Myelin Preparations

Rumsby

Getliffe

Riekkinen

1973

Journal of Neurochemistry

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Stereospecificity of Esterases Hydrolyzing Oxazepam Acetate

Maksay

Tegyey

Ötvös

1978

Journal of Pharmaceutical Sciences

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Preparative resolution and identification of synaptic components of rat neocortex

Garey¹,

Harper²,

Best³

et al. 1972

J. Neurobiol.

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SUMMARYA varity of density gradient centrifugation techniques have been used with varying degrees of success, for the preparative resolution of pinched off nerve endings, i.e. "synaptosomes," and synaptic components from brain homogenates. The physics of such gradients was critically considered from the standpoint of improving the degree of resolution of particulate classes and preserving morphological integrity. A procedure, using continuous linear Ficoll gradients, is presented which yields improved particulate resolution with good preservation of ultrastructural morphology. Further purification of the membraneous fraction yielded relatively high purity preparations of PSM particulates. The PSM particulates were identified as dissociated postsynaptic membranes by three kinds of electron microscope studies. Broad field electron micrographs, which permit assessment of fraction homogeneity, are presented for all fractions.

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Subcellular Localization of Rat Brain Esterases

Abstract: A 109, 000g x 60 in MICROSOME FRACTION WASH MEDIA A, 50, 000g x 120 mimi. 4BjC jD jEJ TRITON X-100 EXTRACT (for starch electrophoresis) Preparation of fractions: Male albino rats weighing 300-400 g were killed by spinal fracture, 456 BERNSOHN ET AL.

Cited by 52 publications

References 0 publications

On the Association of Non‐specific Esterase Activity With Central Nerve Myelin Preparations

On the Association of Non‐specific Esterase Activity With Central Nerve Myelin Preparations

Stereospecificity of Esterases Hydrolyzing Oxazepam Acetate

Preparative resolution and identification of synaptic components of rat neocortex

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