Subcellular optochemical nanobiosensors: probes encapsulated by biologically localised embedding (PEBBLEs)

Clark, Heather A.; Barker, Susan L. R.; Brasuel, Murphy; Miller, Mary; Monson, Eric; Parus, Steve; Shi, Zhu‐Pei; Song, Antonius; Thorsrud, Bjorn A.; Kopelman, Raoul; Ade, Alex; Meixner, Walter; Athey, Brian D.; Hoyer, Marion; Hill, Daniel H.; Lightle, Rhonda; Philbert, Martin A.

doi:10.1016/s0925-4005(98)00212-3

Cited by 133 publications

(107 citation statements)

References 10 publications

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“…As another type of hosting material, polymer or phospholipid also provide a diversity and biocompatible matrix for dye-encapsulation [28]. For instance, the PEBBLEs (Probes Encapsulated By Biologically Localized Embedding) nanosensors, developed by Kopelman et al, were fabricated from polyacrylamide, decylmethacrylate or sol-gel silica [29].…”

Section: Dye-doped Fluorescent Nanoparticlesmentioning

confidence: 99%

Fluorescent nanoparticles for chemical and biological sensing

Liu

Yang

et al. 2011

Sci. China Chem.

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Fluorescent nanoparticles (NPs), including quantum dots (QDs), dye-doped NPs, and rare earth-based NPs, etc., have been a major focus of research and development during the past decade. The impetus behind such endeavors can be attributed to their unique chemical and optical properties, such as bright fluorescence, high photostability, large Stocks shift and flexible processability. The introduction of fluorescent NPs into analytical chemistry has opened up new venues for fluorescent analysis. In this review, we focus on the developments and analytical applications of fluorescent NPs in the chemical and biological sensing of pH, ions, organic compounds, small biological molecules, nucleic acids, proteins, virus and bacteria. The review also points out the in vitro and in vivo imaging application of fluorescent NPs at the cell and body levels. Meanwhile, the advantages of NPs brought field of sensing and signal transductions are also discussed. fluorescence, sensing, quantum dots, dye-doped nanoparticles, rare earth-based nanoparticles

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Section: Dye-doped Fluorescent Nanoparticlesmentioning

confidence: 99%

Fluorescent nanoparticles for chemical and biological sensing

Liu

Yang

et al. 2011

Sci. China Chem.

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“…Moreover, by functionalization with therapeutic antisense DNA, which was explored to overcome the obstacle of multidrug resistance in chemotherapy, the multifunctional nanogel could be constructed to selectively kill drug-resistant cancer cells. Many cellular processes depend on the concentrations of small molecules and ions in the cellular environment, including cations, anions, pH values, amino acids, sugars, and ATP [159,160]. Quantitation of ions or metabolites is important in understanding cell metabolism [161].…”

Section: Aptamer-based Hydrogels For In Vivo Applicationsmentioning

confidence: 99%

Aptamer-Based Hydrogels and Their Applications

Lü

et al. 2015

Aptamers Selected by Cell-Selex for Theranostics

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Hydrogels are water-retainable materials that can absorb a large amount of water. Different stimuli can be used to stimulate the hydrogels with a variety of physical and chemical changes, thus leading to numerous applications in bioanalysis and biomedicine. Aptamers are special types of single-stranded DNA generated by a process called systematic evolution of ligands by exponential enrichment (SELEX). They are able to specifically recognize a wide range of targets which vary from ions, small molecules, to proteins, and even whole cells. Aptamer incorporation has greatly expanded the applications of hydrogels. Due to their unique properties, such as biocompatibility, selective binding, and molecular recognition, these aptamer-based hydrogels can be utilized for target-responsive hydrogel engineering. In this chapter, we discuss a variety of applications of aptamer-based hydrogels, especially in sensing, target capture and separation, control of target release and in vivo applications.

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“…In order to monitor the pH in living cells in vivo, in situ and real time, the pH sensors delivery methods should be efficient and noninvasive. Up to now, the methods for inserting nanosensors into single viable cells mainly include picoinjection, gene gun delivery, liposomal delivery, receptor mediated endocytosis, nonspecific endocytosis and so on [14,15] . The nanosensors were inserted into viable cells through nonspecific endocytosis when the nanosensors were not modified with ligand.…”

Section: Characterization Of the Ratiometric Ph Nanosensorsmentioning

confidence: 99%

Research of the relationship of intracellular acidification and apoptosis in Hela cells based on pH nanosensors

Wang

et al. 2007

SCI CHINA SER B

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In this paper, the relationship of intracellular acidification and apoptosis in Hela cells induced by vincristine sulfate has been studied by use of the ratiometric pH nanosensors that have been developed by our group, employing fluorescein isothiocyanate (FITC) doped as the pH-sensitive dye and Tris(2,2'-bipyidyl) dichlororuthenium(II) hexahydrate (RuBpy) doped as reference dye. The pH change of the Hela cells induced by vincristine sulfate has been monitored in vivo, in situ and real time by use of the ratiometric pH nanosensors. The experimental results show that the pH of the apoptotic Hela cells induced by vincristine sulfate has been acidified from 7.11 to 6.51, and the percentage of intracellular acidification is correlated with the induced concentration and incubation time of the vincristine sulfate. The further study of the percentage of intracellular acidification and the percentage of apoptosis of Hela cells at the same time reveals that apoptosis of Hela cells induced by vincristine sulfate is preceded by intracellular acidification. These results would provide theoretical foundation for the therapy of cancer through interfering the pH of cells by use of vincristine sulfate or other anti-cancer drugs. ratiometric pH nanosensors, vincristine sulfate, Hela cells, intracellular acidification, apoptosis of cellsApoptosis is a programmed cell death process with great active roles in biology, which regulates and controls the physiological process together with mitosis such as development of embryo, differentiation of tissue, immunity and decrepitude of individual and so on [1] . The occurrence of apoptosis involves not only the related genes including survivable genes (e.g. Bcl-2 and Bcl-x) and deadly genes (e.g. C-myc and P53), but also the accommodation of intracellular signal systems such as the concentration of dissociative calcium ion, endogenous endonuclease, intracellular pH and so on [2,3] . Therefore, illustration of the influencing factors and relationships between intracellular signals transmission during the cell apoptosis is all of great importance to understand the evolution of life, the occurrence of disease and the directed disease therapy of drugs. Up to now, many research results [4][5][6] have showed that the cell apoptosis was correlated with the change of intracellular pH. The intracellular acidification has become a notable feature

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Subcellular optochemical nanobiosensors: probes encapsulated by biologically localised embedding (PEBBLEs)

Cited by 133 publications

References 10 publications

Fluorescent nanoparticles for chemical and biological sensing

Fluorescent nanoparticles for chemical and biological sensing

Aptamer-Based Hydrogels and Their Applications

Research of the relationship of intracellular acidification and apoptosis in Hela cells based on pH nanosensors

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