Subcellular sites of calcium accumulation and relationships with conidiation in Penicillium cyclopium

Ugalde, Unai; Pitt, D.

doi:10.1016/s0007-1536(84)80173-4

Cited by 14 publications

(2 citation statements)

References 29 publications

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“…Ugalde & Pitt (1986) showed that as little as 30 s exposure of P. cycfopium to 9 mM-Ca2+ stimulated subsequent differentiation of conidiophores as efficiently as if the fungus was left in the Ca2+ environment. The Ca2+ uptake was biphasic, initial binding to hyphae being followed by active transport of the cation to the subcellular sites described earlier (Ugalde & Pitt, 1984). However, the study did not extend to include redistribution into spores.…”

Section: Discussionmentioning

confidence: 87%

Submerged Fermentation of Penicillium paxilli Biosynthesizing Paxilline, a Process Inhibited by Calcium-induced Sporulation

Ibba¹,

Taylor²,

Weedon³

et al. 1987

Microbiology

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A submerged fermentation process for the production of the tremorgenic mycotoxin paxilline by Penicillium paxilli has been developed. The fungus did not sporulate and accumulated paxilline to 1.5 % (w/w) in freeze-dried cells within 6 d in a 60 1 stirred fermenter. Induction of extensive differentiation of conidiophores and profuse sporulation by adding 2% (w/v) CaCl,. 2 H 2 0 to the medium at batching reduced paxilline yield by 97 %. Paxilline biosynthesis occurred when the glucose in the medium had been exhausted, implying that carbon catabolite repression may be involved in the biosynthesis of this alkaloid, even when calcium-induced sporulation inhibits or delays the onset of paxilline biosynthesis. Sporulation-induced inhibition of indole-terpenoid alkaloid biosynthesis in P. paxilli contrasts with the situation in some other penicillia elaborating indole alkaloids and allows disassociation of aspects of secondary metabolite biosynthesis from growth-associated differentiation, which formerly seemed to be linked.

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Section: Discussionmentioning

confidence: 87%

Submerged Fermentation of Penicillium paxilli Biosynthesizing Paxilline, a Process Inhibited by Calcium-induced Sporulation

Ibba¹,

Taylor²,

Weedon³

et al. 1987

Microbiology

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“…This process of differentiation in shake flasks appeared to be fairly synchronous and took about 7 hr for completion. Using the radioisotope 45Ca, Ugalde and Pitt (1984) demonstrated that calcium is bound principally to membrane components of the cytoplasm such as the plasma membrane and mitochondria. For these studies, density-gradient centrifugation in silica-sol gradients was used to fractionate organelles liberated from mycelium homogenized with acid-washed glass beads in a Braun MSK cell homogenizer.…”

Section: Growth In Liqid Mediamentioning

confidence: 99%

Penicillium and Acremonium

Peberdy¹

1987

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v Foreword Biotechnology is a word that was originally coined to describe the new processes which could be derived from our ability to manipulate, in vitro, the genetic material common to all organisms. I t has now become a generic term encompassing all "applications" of living systems, including the more traditional fermentation and agricultural industries. Recombinant DNA technology has opened up new opportunities for the exploitation of microorganisms and animal and plant cells as producers or modifiers of chemical and biological products.This series of handbooks deals exclusively with microorganisms which are at the forefront of the new technologies and brings together in each of its volumes the background information necessary to appreciate the historical development of the organisms making up a particular genus, the degree to which molecular biology has opened up new opportunities, and the place they occupy in today's biotechnology industry. Our aim was to make this primarily a practical approach, with emphasis on methodology, combining for the first time information which has largely been spread across a wide literature base or only touched upon briefly in review articles. Each handbook should provide the reader with a source text, from which the importance of the genus to his or her work can be identified, and a practical guide to the handling and exploitation of the organisms included.It is perhaps apt that Volume I of the series should deal with a group of organisms involved in producing chemicals which have dominated the fermentation and pharmaceutical industries for the past forty years-the antibiotics. In terms of the degree to which molecular biology has made an impact on this genus to date, it is clear that the major rewards are still to come.

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Morphology and Physiology of Penicillium and Acremonium

Moss

1987

Penicillium and Acremonium

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Subcellular sites of calcium accumulation and relationships with conidiation in Penicillium cyclopium

Cited by 14 publications

References 29 publications

Submerged Fermentation of Penicillium paxilli Biosynthesizing Paxilline, a Process Inhibited by Calcium-induced Sporulation

Submerged Fermentation of Penicillium paxilli Biosynthesizing Paxilline, a Process Inhibited by Calcium-induced Sporulation

Penicillium and Acremonium

Morphology and Physiology of Penicillium and Acremonium

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