Subchronic feeding study of grain from herbicide-tolerant maize DP-Ø9814Ø-6 in Sprague-Dawley rats

Appenzeller, Laura M.; Munley, Susan M.; Hoban, Denise; Sykes, Greg P.; Malley, Linda A.; Delaney, Bryan

doi:10.1016/j.fct.2009.06.014

Cited by 30 publications

(15 citation statements)

References 54 publications

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“…This finding is consistent with those of previous studies in which no biologically significant differences in organ indices (McNaughton et al, 2008) or serum biochemical parameters (Appenzeller et al, 2009) were observed between animals fed diets prepared with GM or non-GM grain. This suggests that a diet prepared from PTC has no unintended effects on serum biochemical parameters and organ weight as compared to a CC-based diet.…”

Section: Resultssupporting

confidence: 93%

Effect of Dietary Phytase Transgenic Corn on Physiological Characteristics and the Fate of Recombinant Plant DNA in Laying Hens

Gao

Zhao

et al. 2014

Asian Australas. J. Anim. Sci

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The study aimed to evaluate the potential effects of feeding with phytase transgenic corn (PTC) on organ weight, serum biochemical parameters and nutrient digestibility, and to determine the fate of the transgenic DNA in laying hens. A total of 144 50-week-old laying hens were grouped randomly into 2 treatments, with 8 replicates per treatment and 9 hens per replicate. Each treatment group of hens was fed with diets containing 62.4% non-transgenic conventional corn (CC) or PTC for 16 weeks. The phytase activity for CC was 37 FTU/kg of DM, whereas the phytase activity for PTC was 8,980 FTU/kg of DM. We observed that feeding PTC to laying hens had no adverse effect on organ weight or serum biochemical parameters (p>0.05). A fragment of a poultry-specific ovalbumin gene (ov) was amplified from all tissues of hens showing that the DNA preparations were amenable to PCR amplification. Neither the corn-specific invertase gene (ivr) nor the transgenic phyA2 gene was detected in the breast muscle, leg muscle, ovary, oviduct and eggs. The digestibility data revealed no significant differences between the hens that received the CC- and PTC-based diets in the digestibility of DM, energy, nitrogen and calcium (p>0.05). Phosphorus digestibility of hens fed the PTC-based diet was greater than that of hens fed the CC-based diet (58.03% vs 47.42%, p<0.01). Based on these results, it was concluded that the PTC had no deleterious effects on the organ weight or serum biochemical parameters of the laying hens. No recombinant phyA2 gene was detected in muscle tissues and reproductive organs of laying hens. The novel plant phytase was efficacious in improving the phosphorus digestibility of laying hens.

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Section: Resultssupporting

confidence: 93%

Effect of Dietary Phytase Transgenic Corn on Physiological Characteristics and the Fate of Recombinant Plant DNA in Laying Hens

Gao

Zhao

et al. 2014

Asian Australas. J. Anim. Sci

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“…There were no significant differences between the groups in terms of clinical observations, haematological parameters, biochemical markers and absolute or relative organ weights. Histopathology revealed a similar prevalence of anomalies among the three groups which are regarded as spontaneous, in agreement with data from previous feeding trials involving crops engineered for herbicide tolerance and pest resistance (Appenzeller et al. , 2009; Hammond et al.…”

Section: Discussionsupporting

confidence: 89%

Mice fed on a diet enriched with genetically engineered multivitamin corn show no sub‐acute toxic effects and no sub‐chronic toxicity

Arjó¹,

Capell²,

Matías‐Guiu³

et al. 2012

Plant Biotechnology Journal

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SummaryMultivitamin corn is a novel genetically engineered variety that simultaneously produces high levels of b-carotene, ascorbate and folate, and therefore has the potential to address simultaneously multiple micronutrient deficiencies caused by the lack of vitamins A, B9 and C in developing country populations. As part of the development process for genetically engineered crops and following European Food Safety Authority (EFSA) recommendations, multivitamin corn must be tested in whole food ⁄ feed sub-chronic animal feeding studies to ensure there are no adverse effects, and potential allergens must be identified. We carried out a 28-day toxicity assessment in mice, which showed no short-term sub-acute evidence of dietrelated adverse health effects and no difference in clinical markers (food consumption, body weight, organ ⁄ tissue weight, haematological and biochemical blood parameters and histopathology) compared to mice fed on a control diet. A subsequent 90-day sub-chronic feeding study again showed no indications of toxicity compared to mice fed on control diets. Our data confirm that diets enriched with multivitamin corn have no adverse effects on mice, do not induce any clinical signs of toxicity and do not contain known allergens.

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“…Long-term and multigenerational feeding studies Long-term feeding studies. In a 90-day or 13-weeks feeding studies on rats, several groups have reported that there were no effects on male reproductive organs such as testes, epididymides and prostate in rodents compared with rats receiving non-GM food in long-term study when the diet was treated with different GM foods (Hammond et al, 2006;MacKenzie et al, 2007;Schroder et al, 2007;Appenzeller et al, 2008Appenzeller et al, , 2009aAppenzeller et al, and 2009bDelaney et al, 2008b;Healy et al, 2008;He et al, 2008 and No apparent differences in percentages of testicular cell populations (haploid, diploid and tetraploid).…”

Section: Velimirov Et Al (2008)mentioning

confidence: 99%

“…In the 90-day or 13-week feeding studies in rats, no statistical difference was uncovered in either the relative body and ovary weight of rats fed transgenic corns (Hammond et al, 2006;Healy et al, 2008), maize (MacKenzie et al, 2007;He et al, 2009;Appenzeller et al, 2009a and2009b), rice (Schroder et al, 2007) or soybeans (Appenzeller et al, 2008;Delaney et al, 2008b); and there were no histopathologic lesions in ovaries from rats fed DAS-59122-7 maize (Cry34Ab1 and Cry35Ab1 proteins; He et al, 2008). Furthermore, Malley et al (2007) reported higher mean uterus weight during the estrous stage of rats fed maize DAS-59122-7 (Cry34Ab1 and Cry35Ab1 proteins) or 5002B (commercial rodent diets) v. the 33R77 group (non-transgenic reference maize grain), 091 group (non-transgenic nearisogenic maize grain) or 5002A (commercial rodent diets); however, this might be due to the fact that the proportion of rats in proestrus and estrus in the 59122 and 5002B groups was greater than that in the 5002A, 091 and 33R77 maize grain groups, in which a greater proportion of rats were in metestrus and diestrus.…”

Section: Introductionmentioning

confidence: 99%

Do genetically modified crops affect animal reproduction? A review of the ongoing debate

Zhang

Shi

2011

Animal

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In the past few years, genetically modified (GM) crops aimed at producing food/feed that became part of the regular agriculture in many areas of the world. However, we are uncertain whether GM food and feed can exert potential adverse effects on humans or animals. Of importance, the reproductive toxicology of GM crops has been studied using a number of methods, and by feeding GM crops to a number species of animals to ensure the safety assessment of GM food and feed. It appears that there are no adverse effects of GM crops on many species of animals in acute and short-term feeding studies, but serious debates of effects of long-term and multigenerational feeding studies remain. The aims of this review are to focus on the latest (last 3 to 4 years) findings and debates on reproduction of male and female animals after feeding daily diets containing the GM crops, and to present the possible mechanism(s) to explain their influences.

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Subchronic feeding study of grain from herbicide-tolerant maize DP-Ø9814Ø-6 in Sprague-Dawley rats

Cited by 30 publications

References 54 publications

Effect of Dietary Phytase Transgenic Corn on Physiological Characteristics and the Fate of Recombinant Plant DNA in Laying Hens

Effect of Dietary Phytase Transgenic Corn on Physiological Characteristics and the Fate of Recombinant Plant DNA in Laying Hens

Mice fed on a diet enriched with genetically engineered multivitamin corn show no sub‐acute toxic effects and no sub‐chronic toxicity

Do genetically modified crops affect animal reproduction? A review of the ongoing debate

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