Subclinical, chronic intramammary infection lowers steroid concentrations and gene expression in bovine preovulatory follicles

Lavon, Y.; Leitner, Gabriel; Klipper, Eyal; Moallem, U.; Meidan, Rina; Wolfenson, D.

doi:10.1016/j.domaniend.2010.09.004

Cited by 66 publications

(64 citation statements)

References 41 publications

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“…These might reduce GnRH secretion which, in turn, will lead to low and delayed preovulatory LH surge [55]. Supporting this, the low follicular steroid production found in the preovulatory follicular fluid was associated with low expression of major steroidogenic enzymes and LH receptor in both theca and granulosa cells [64].…”

Section: Effects Of Mastitismentioning

confidence: 85%

“…Lavon et al [55] found that 30% of subclinical, mastitic cows exhibit delayed ovulation which may interfere with fertilization and lower conception rate. The mechanism associated with delayed ovulation involves low concentrations of both follicular estradiol and androstenedione [64], and low circulating estradiol close to estrus. These might reduce GnRH secretion which, in turn, will lead to low and delayed preovulatory LH surge [55].…”

Section: Effects Of Mastitismentioning

confidence: 99%

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Comparing the effects of heat stress and mastitis on ovarian function in lactating cows: basic and applied aspects

Roth

Wolfenson

2016

Domestic Animal Endocrinology

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Section: Effects Of Mastitismentioning

confidence: 85%

Section: Effects Of Mastitismentioning

confidence: 99%

Comparing the effects of heat stress and mastitis on ovarian function in lactating cows: basic and applied aspects

Roth

Wolfenson

2016

Domestic Animal Endocrinology

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“…While infection of the ovary itself is a rare event, bacterial infections of the reproductive tract or mammary gland are common following parturition in dairy cattle. These infections perturb ovarian antral follicle growth and function [3,5,6]. During uterine disease, LPS is evident within follicular fluid in vivo, and LPS elicits an inflammatory response from granulosa cells in antral follicles or cumulusoocyte complexes in vitro, which also perturbs their endocrine function [4,14].…”

Section: Discussionmentioning

confidence: 99%

“…The incidence of mastitis is at least as common as metritis, and mastitis is also associated with reduced fertility [2]. Metritis and mastitis perturbation result in several aspects of ovarian function, including retarded follicular growth, reduced circulating and intrafollicular estradiol concentrations, altered duration of luteal phases, and disruption of ovarian cyclic activity [3][4][5][6]. However, infections of the uterus and mammary gland not only cause infertility at the time of infection but reduced fecundity also persists even after resolution of disease [1,7].…”

Section: Introductionmentioning

confidence: 99%

“…One link between bacterial infection and ovarian dysfunction is the accumulation of LPS in the follicular fluid of animals with metritis in vivo [4]. Estradiol is reduced in granulosa cells cultured with LPS [5], while animals with mastitis have altered granulosa cell gene expression and lower follicular estradiol [6]. The innate immune system relies on pattern recognition receptors on host mammalian cells to detect microbe-or pathogen-associated molecular patterns, including LPS [8].…”

Section: Introductionmentioning

confidence: 99%

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Lipopolysaccharide Reduces the Primordial Follicle Pool in the Bovine Ovarian Cortex Ex Vivo and in the Murine Ovary In Vivo1

Bromfield

Sheldon

2013

Biology of Reproduction

110

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Infections of the uterus or mammary gland with Gram-negative bacteria cause infertility in cattle, not only during disease but also for some time afterward. Even though these infections are in organs distant from the ovary, metritis and mastitis perturb antral follicle development and function in vivo. Although granulosa cells from antral follicles express toll-like receptor 4 (TLR4), and detect and mount an inflammatory response to lipopolysaccharide (LPS) from Gram-negative bacteria, it is not known whether LPS impacts preantral follicle development. The present study tested the hypothesis that LPS perturbs the development of primordial ovarian follicles. Exposure of bovine ovarian cortex ex vivo to LPS reduced the primordial follicle pool associated with increased primordial follicle activation. Ovarian cortex culture supernatants accumulated the inflammatory mediators IL-1beta, IL-6, and IL-8 in an LPS concentration-dependent manner. In addition, LPS exposure modulated key intracellular regulators of follicle activation with loss of the primordial follicle PTEN and cytoplasmic translocation of FOXO3. Acute exposure of mice in vivo to LPS also reduced the primordial follicle pool associated with increased follicle atresia. The increased follicle atresia was TLR4-dependent as Tlr4-deficient mice were insensitive to LPS-mediated follicle atresia. However, LPS did not affect the diameter of individually cultured bovine secondary follicles or their enclosed oocytes. In conclusion, LPS reduced the primordial ovarian follicle pool in the bovine ovarian cortex ex vivo and in the murine ovary in vivo. These observations provide an insight into how bacterial infections distant from the ovary have long term effects on fertility.

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Stress‐induced alterations in oocyte transcripts are further expressed in the developing blastocyst

Roth

2018

Molecular Reproduction Devel

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The oocyte achieves its developmental competence through the lengthy process of folliculogenesis. It can therefore potentially be exposed to various stressors while enclosed in the follicle. Oocyte maturation relies mainly on maternal sources. These include nuclear, cytoplasmic, and molecular maturation, which involve DNA and RNA organization. Maternal transcripts are dominant through the first embryonic cleavages, up until embryonic genome activation. Thus, it is suggested that any perturbations during oocyte storage, in particular of the maternal transcripts, might lead to genetic and/or epigenetic changes, which might be further expressed in the developing embryo. The review discusses the effects of three representative stressors-environmental heat stress, endocrine-disrupting compounds (phthalates), and inflammatory stress (mastitis)-shown to be involved in reduced fertility. The review highlights the carryover response from the oocyte to the developing embryo; it includes intracellular and molecular disruptive mechanisms with an emphasis on maternal transcripts. The review provides insights into the oocyte's cellular and molecular responses with an emphasis on the effects of various stressors on the maternal (nuclear and mitochondrial) transcripts and the association with embryonic development. A comparison between stressors might clarify, at least in part, a few open questions. For instance, (a) whether stress-induced alterations share the same mechanism and if so (b) whether this mechanism involves alterations of maternal transcripts; (c) whether stress-induced alterations in the maternal transcript are further expressed at the developing blastocyst stage, that is, after embryonic genome activation.

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Subclinical, chronic intramammary infection lowers steroid concentrations and gene expression in bovine preovulatory follicles

Cited by 66 publications

References 41 publications

Comparing the effects of heat stress and mastitis on ovarian function in lactating cows: basic and applied aspects

Comparing the effects of heat stress and mastitis on ovarian function in lactating cows: basic and applied aspects

Lipopolysaccharide Reduces the Primordial Follicle Pool in the Bovine Ovarian Cortex Ex Vivo and in the Murine Ovary In Vivo1

Stress‐induced alterations in oocyte transcripts are further expressed in the developing blastocyst

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