Suberosin Alleviates Sepsis-Induced Lung Injury in A Rat Model of Cecal Ligation and Puncture

Uzunçakmak, Sevgi Karabulut; Halıcı, Zekai; Karakaya, Songül; Kutlu, Zerrin; Sağlam, Yavuz Selim; Bolat, İsmail; Aydin, Pelin; Kılıç, Ceyda Sibel

doi:10.1080/08941939.2022.2136802

Cited by 10 publications

(5 citation statements)

References 33 publications

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“…As described in previous studies (21), cecal ligation and puncture (CLP) was used in this study to prepare a rat sepsis model. The rats were anesthetized with 4% sevoflurane.…”

Section: Cecal Ligation and Puncture Operationmentioning

confidence: 99%

The Protective Effect of Dexmedetomidine on the Liver Injury in Sepsis Through Inhibition of Necroptosis

Meng,

Zhang

et al. 2024

Shock

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Background Sepsis-induced liver injury leads to extensive necroptosis in hepatocytes, which is the main factor of liver dysfunction. This study aims to investigate the protective effect of dexmedetomidine (DEX) on septic liver and to explore whether its molecular mechanism is related to the modulation of necroptosis. Methods The model of septic liver injury was induced by cecal ligation and puncture (CLP) in rats. DEX and necrostatin-1(Nec-1), a specific antagonist of necroptosis, were administered 1 h before CLP. The levels of arterial blood gas (ABG), serum AST and ALT were measured at 6, 12 and 24 h after CLP. The survival rate was observed 24 h after CLP. Liver pathological changes and apoptosis, the contents of IL-6 and TNF-α in liver tissue homogenates, the ROS content in liver tissue, and the expression levels of RIP1, RIP3, MLKL and HMGB1 were detected. Results At 6, 12, and 24 h after CLP, the levels of ALT and AST levels increased, and liver enzyme levels gradually increased with the progression of sepsis. In ABG analysis, PaO2 gradually decreased and lactic acid concentration gradually increased during these three time periods. The morphological impairment of liver tissues, increased apoptosis, elevated inflammatory factors (IL-6 and TNF-α), increased ROS level and necroptosis components (RIP1, RIP3, MLKL and HMGB1) were all observed in sepsis rats. However, these injuries can be ameliorated by pretreatment with DEX. Meanwhile, Nec-1 pretreatment also reduced the expression of RIP1, RIP3, MLKL, HMGB1, and ROS level. Conclusion Our study suggests that DEX alleviates septic liver injury, and the mechanism is associated with the inhibition of necroptosis.

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“…As described in previous studies (21), cecal ligation and puncture (CLP) was used in this study to prepare a rat sepsis model. The rats were anesthetized with 4% sevoflurane.…”

Section: Cecal Ligation and Puncture Operationmentioning

confidence: 99%

The Protective Effect of Dexmedetomidine on the Liver Injury in Sepsis Through Inhibition of Necroptosis

Meng,

Zhang

et al. 2024

Shock

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“…The abdomen is closed properly. 16 , 17 The aim of the CLP method is to puncture the cecal barrier, leading to peritoneal infection. Perforation of the cecum and exposure of the intestinal contents leads to bacterial contamination, which causes bacterial peritonitis.…”

Section: Experimental Modelsmentioning

confidence: 99%

Experimental Sepsis Models: Advantages and Limitations

Aydın,

Bekmez

2024

The Eurasian Journal of Medicine

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Sepsis is a major health problem that causes millions of deaths worldwide every year. Due to the complexity of its pathophysiology, there is no clear treatment method for it. Existing treatments impose an additional financial burden on the health systems of countries every year. Clinical and preclinical studies are continuously being conducted in order to prevent the development of sepsis, treat patients with sepsis, reduce mortality, and solve the socioeconomic problems that arise from it. However, it is not possible to directly test every study and potential new treatment in humans. Preclinical studies enable an understanding of pathophysiological events and the development of targeted therapies. For this purpose, many experimental sepsis models have been and continue to be applied. The extent to which these models can reflect the human sepsis condition is an important issue that needs to be emphasized. Each method has different strengths and weaknesses. Researchers should choose the most appropriate experimental model according to the characteristics of the experiments they plan and, if possible, conduct their studies on different models.

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“…Sepsis, commonly known as systemic inflammatory response syndrome, arises from bacterial infections or other factors that trigger immune responses in the host. 44 In sepsis, there is an overproduction of oxygen-free radicals, leading to an imbalance in the natural scavenging mechanisms. These processes are implicated in microvascular dysfunction, followed by organ dysfunction.…”

Section: Introductionmentioning

confidence: 99%

Aquaporins: Potential Targets in Inflammatory Diseases

Bozkurt,

Halici,

Yayla

2024

The Eurasian Journal of Medicine

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Inflammation involves a long chain of molecular reactions and cellular activity designed to repair tissue damaged by various causes. The inflammatory process and its complex mechanisms have recently become a focus of interest for many researchers. After the onset of inflammation, various adverse conditions that initiate the inflammatory response need to be addressed; however, failure to limit the inflammatory reaction may result in the damage or destruction of host cells. Therefore, inflammatory reactions play a role in many different diseases. Aquaporins (AQPs), commonly referred to as water channels, are protein channels responsible for forming pores in the membranes of biological cells. Their main function is to aid in the movement of water between cells. Aquaporins not only regulate transepithelial fluid transport across membranes but also play a role in regulating essential events crucial for the inflammatory response. Aquaporins have been shown in many studies to have important roles in inflammatory diseases. This clearly indicates that AQPs may be potential targets for inflammatory diseases. This review summarizes the research to date on the structure and function of AQPs and provides an update on the relationship between AQPs and various human inflammatory diseases.

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Suberosin Alleviates Sepsis-Induced Lung Injury in A Rat Model of Cecal Ligation and Puncture

Cited by 10 publications

References 33 publications

The Protective Effect of Dexmedetomidine on the Liver Injury in Sepsis Through Inhibition of Necroptosis

The Protective Effect of Dexmedetomidine on the Liver Injury in Sepsis Through Inhibition of Necroptosis

Experimental Sepsis Models: Advantages and Limitations

Aquaporins: Potential Targets in Inflammatory Diseases

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