Subfield- and layer-specific changes in parvalbumin, calretinin and calbindin-D28k immunoreactivity in the entorhinal cortex in Alzheimer's disease

Mikkonen, Mia; Alafuzoff, Irina; Tapiola, Tero; Miettinen, Reijo

doi:10.1016/s0306-4522(99)00047-0

Cited by 101 publications

(85 citation statements)

References 56 publications

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“…The human AD brains showed the reduced expression of calcium-binding proteins (Iacopino and Christakos, 1990;Mikkonen et al, 1999). In the present study, Western blotting indicated that calbindin (CB) expression was significantly reduced in the whole cerebrum homogenate of Tg-hCTF99/B6 at 14 -16 months versus the non-transgenic control (Fig.…”

Section: Expression Of Calcium-binding Protein In the Tg-bctf99/b6 Brainmentioning

confidence: 76%

“…Both Tg-hCTF99/B6 and Tg-APP/B6 mice showed reduced calbindin expression in the dentate gyrus. Reduced calbindin expression is also detected in aged Tg2576 mice, not in young Tg2576 mice (our unpublished data), and reduced calbindin expression is known to be a histological feature of the AD brain (Iacopino and Christakos, 1990;Mikkonen et al, 1999). Third, our preliminary data show that the biochemical and behavioral phenotypes of both homozygote and heterozygote of Tg-hCTF99/B6 mice are produced progressively and age-dependently, but only in aged animals, though phenotypes displayed by homozygous Tg-hCTF99/B6 are generally stronger than those of heterozygote.…”

Section: Discussionmentioning

confidence: 99%

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Progressive neuronal loss and behavioral impairments of transgenic C57BL/6 inbred mice expressing the carboxy terminus of amyloid precursor protein

Lee

Song

et al. 2006

Neurobiology of Disease

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Section: Expression Of Calcium-binding Protein In the Tg-bctf99/b6 Brainmentioning

confidence: 76%

Section: Discussionmentioning

confidence: 99%

Progressive neuronal loss and behavioral impairments of transgenic C57BL/6 inbred mice expressing the carboxy terminus of amyloid precursor protein

Lee

Song

et al. 2006

Neurobiology of Disease

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“…Neurons expressing the calcium binding protein calretinin have also been reported to be affected in the most degenerated brain regions of AD patients [13,14]. Briefly, the density of calretinin (CR) neurons, as well as the dendritic immunostaining, was reduced in the entorhinal cortex of severe AD cases [14].…”

Section: Introductionmentioning

confidence: 99%

Calretinin Interneurons are Early Targets of Extracellular Amyloid-β Pathology in PS1/AβPP Alzheimer Mice Hippocampus

Baglietto‐Vargas

Moreno-González

Sánchez-Varo

et al. 2010

JAD

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Abstract. Specific neuronal networks are preferentially affected in the early stages of Alzheimer's disease (AD). The distinct subpopulations of hippocampal inhibitory GABAergic system have been shown to display differential vulnerability to neurodegeneration in AD. We have previously reported a substantial loss of SOM/NPY interneurons, whereas those expressing parvalbumin were unaltered, in the hippocampus of 6 month-old PS1/AβPP transgenic mice. In the present study, we now investigated the pathological changes of hippocampal calretinin (CR) interneurons in this PS1/AβPP model from 2 to 12 months of age. The total number of CR-immunoreactive inhibitory cells was determined by stereology in CA1 and CA2/3 subfields. Our findings show a substantial decrease (35%-45%) of CR-positive interneurons in both hippocampal subfields of PS1/AβPP mice at very early age (4 months) compared to age-matched control mice. This decrease was accompanied by a reduced CR mRNA content as determined by quantitative RT-PCR. However, the number of another hippocampal CR-positive population (belonging to Cajal-Retzius cells) was not affected. The selective early loss of CR-interneurons was parallel to the appearance of extracellular Aβ deposits, preferentially in CR-axonal fields, and the formation of dystrophic neurites. This specific GABAergic subpopulation plays a crucial role in the generation of synchronous rhythmic activity in hippocampus by controlling other interneurons. Therefore, early alterations of hippocampal inhibitory functionality in AD, caused by select CR-cells neurodegeneration, could result in cognitive impairments seen in initial stages of the disease.

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“…En la literatura revisada se halló apenas una mención breve sobre el aumento en la intensidad de la inmunorreactividad a PV así como a CB y CR en algunas neuronas corticales en casos de enfermedad de Alzheimer (44). Sin embargo, no hay datos similares en la mayoría de publicaciones revisadas sobre esta patología.…”

Section: Tipos De Virus Y Vías De Inoculación De La Rabiaunclassified

“…Puede tratarse únicamente de inhibición de la expresión de la proteína aunque la célula siga intacta. Sin embargo, la reducción en el tamaño del soma y las neuritas es un factor indicativo de atrofia y pérdida neuronal (21,24,44).…”

Section: Tipos De Virus Y Vías De Inoculación De La Rabiaunclassified

Efecto de la infección por el virus de la rabia sobre la expresión de parvoalbúmina, calbindina y calretinina en la corteza cerebral de ratones.

Fernández

Yepes

Gómez³

et al. 2004

biomedica

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Algunas manifestaciones clínicas de la rabia, así como los resultados de experimentos con cultivos celulares y animales de laboratorio han llevado a sugerir que el virus de la rabia afecta la neurotransmisión gabaérgica. En la corteza cerebral existen diferentes tipos de neuronas que sintetizan el neurotransmisor GABA. Éstas se pueden identificar con marcadores neuronales, entre los que se destacan tres proteínas ligadoras de calcio: la parvoalbúmina (PV), la calbindina (CB) y la calretinina (CR). El virus de la rabia se disemina a través de la corteza cerebral pero se desconocen sus posibles efectos citopáticos sobre las neuronas gabaérgicas. Para evaluar el efecto de la rabia sobre estas neuronas, se estudió mediante inmunohistoquímica la expresión de PV, CB y CR en la corteza frontal de ratones normales y ratones infectados con virus 'calle' o virus 'fijo' de la rabia. La PV se expresó en neuronas multipolares dispersas regularmente entre las capas II y VI, y en botones sinápticos que bordeaban el soma de las neuronas piramidales. La inmunorreactividad a CB se manifestó en dos franjas corticales: la primera, en las capas supragranulares II y III en neuronas con somas redondeados e inmersos en un neuropilo intensamente marcado; la segunda, en las capas infragranulares V y VI en neuronas multipolares dispersas y rodeadas por un neuropilo menos reactivo. La CR se expresó en neuronas bipolares con somas fusiformes distribuidas en las seis capas corticales, pero concentradas principalmente en las capas II y III. Hubo una característica común en las muestras infectadas con los dos tipos de virus: la inmunotinción a PV fue más intensa que en las muestras normales. La infección derivada del virus 'calle' no causó alteraciones adicionales en la expresión de las tres proteínas. En contraste, la infección con virus 'fijo' produjo una reducción notable del número de neuronas CB+, así como de la inmunorreactividad a CB en el neuropilo de la corteza frontal. Además, provocó una disminución significativa del tamaño de las neuronas CR+ en la corteza del cíngulo. Estos resultados aportan evidencia histológica en apoyo de la hipótesis según la cual las neuronas gabaérgicas son afectadas por el virus de la rabia.Palabras clave: rabia, parvoalbúmina, calbindina, calretinina, GABA. Effect of rabies virus infection on the expression of parvalbumin, calbindin and calretinin in mouse cerebral cortexSome clinical features of rabies and experimental evidence from cell culture and laboratory animals suggest impairment of gabaergic neurotransmission. Several types of gabaergic neurons occur in the cerebral cortex. They can be identified by three neuronal markers: the calcium binding proteins (CaBPs) parvalbumin (PV), calbindin (CB) and calretinin (CR). Rabies virus spreads throughout the cerebral cortex; however, rabies cytopathic effects on gabaergic neurons are unknown. The expression of calcium-binding proteins (CaBPs) parvalbumin (PV), calbindin (CB) and calretinin (CR) was studied in the frontal cortex of mice. The effect of gabaer...

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Subfield- and layer-specific changes in parvalbumin, calretinin and calbindin-D28k immunoreactivity in the entorhinal cortex in Alzheimer's disease

Cited by 101 publications

References 56 publications

Progressive neuronal loss and behavioral impairments of transgenic C57BL/6 inbred mice expressing the carboxy terminus of amyloid precursor protein

Progressive neuronal loss and behavioral impairments of transgenic C57BL/6 inbred mice expressing the carboxy terminus of amyloid precursor protein

Calretinin Interneurons are Early Targets of Extracellular Amyloid-β Pathology in PS1/AβPP Alzheimer Mice Hippocampus

Efecto de la infección por el virus de la rabia sobre la expresión de parvoalbúmina, calbindina y calretinina en la corteza cerebral de ratones.

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