Subgenomic viral DNA species synthesized in simian cells by human and simian adenoviruses

Daniell, Ellen

doi:10.1128/jvi.37.2.620-627.1981

Cited by 8 publications

(2 citation statements)

References 42 publications

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“…Differences in this region of the genome may relate to the early replicative block of EAds in conventional cell lines and the possible complementation of defective early EAd genes by the AdS genes expressed in 293 cells (23). Incomplete adenovirus particles have been noted for several years, and their nature, origin, and significance have been discussed in relation to theories of Ad DNA replication (4,7,12,16,17,20,25). Our observations are similar to those reported in studies of incomplete particles of other Ad types.…”

Section: Ad2 Genome Areasmentioning

confidence: 99%

Cloning and physical mapping of enteric adenoviruses (candidate types 40 and 41)

Takiff¹,

Reinhold²,

Garon³

et al. 1984

J Virol

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We have studied the DNAs of fastidious enteric adenoviruses recovered from the stools of infants with gastroenteritis. By endonuclease analysis, the strains examined represent candidate adenovirus types 40 and 41, which are thought to comprise new adenovirus subgroups F and G. Cloning of DNA from representative enteric adenovirus isolates, together with hybridization and subcleavage analysis, permitted the mapping of restriction enzyme cleavage sites. Although the restriction profiles are different for the two strains, they appear to have several cleavage sites in common. Cross hybridization studies show considerable homology between the subgroup F and G strains but much less homology to adenovirus 2. In addition, regions on both ends of enteric adenovirus genomes (map units, 2.9 to 11.3 and 75 to 100) possess little or no homology to adenovirus 2. Restriction enzyme digests reveal submolar fragments that map to the terminal regions of the genome. Electron micrographic studies of denatured and renatured DNA strands suggest that the submolar fragments may derive from cleavage of defective molecules. Inverted terminal repeat sequences were shown to comprise 0 to 3.2% of the length of complete (_22 megadaltons) enteric adenovirus DNA molecules but 4 to 69% of incomplete-length (<22-megadalton) molecules.

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Section: Ad2 Genome Areasmentioning

confidence: 99%

Cloning and physical mapping of enteric adenoviruses (candidate types 40 and 41)

Takiff¹,

Reinhold²,

Garon³

et al. 1984

J Virol

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“…The ∆E3 virus, Ad5rhFLSC, has been described elsewhere [20]. In brief, 2800 base pairs in the E3 region of Adhr404 [21] were replaced with rhFLSC. The mutant virus dl1520 contains an 827-bp deletion in the region encoding the 55kDa protein [22].…”

Section: Virusesmentioning

confidence: 99%

E4orf1 Suppresses E1B-Deleted Adenovirus Vaccine-Induced Immune Responses

et al. 2022

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As demonstrated by the recent COVID pandemic, vaccines can reduce the burden arising from infectious agents. Adenoviruses (Ads) with deletion of the early region 1B55K (ΔE1B Ad) are currently being explored for use in vaccine delivery. ΔE1B Ads are different from Ads with deletions in early region 1 and early region 3 (ΔE1/E3) used in most Ad vaccine vectors in that they contain the Ad early region 1A (E1A), and therefore the ability to replicate. Common to almost all Ads that are being explored for clinical use is the Ad early region 4 (E4). Among the E4 genes is open reading frame 1 (E4orf1), which mediates signals through the PI3-kinase/Akt pathway that is known to modulate immune responses. This suggests that E4orf1 might also modulate immune responses, although it has remained unexplored in ΔE1B Ad. Here, we show that cells infected with an E1B55K and E4orf1-deleted (ΔE41) Ad exhibited reduced levels of phosphorylated Akt (Ser473 and Thr308)) and expressed different intrinsic innate immune cytokines from those induced in cells infected with an E4orf1-containing, ΔE1B parental Ad that exhibited elevated levels of phosphorylated Akt. Rhesus macaques immunized with a ΔE41 Ad that expressed rhFLSC (HIV-1BaL gp120 linked to rhesus CD4 D1 and D2), exhibited higher levels of rhFLSC-specific interferon γ-producing memory T-cells, higher titers of rhFLSC-specific IgG1 binding antibody in serum, and antibodies able to mediate antibody-dependent cellular cytotoxicity (ADCC) with greater killing capacity than the ΔE1B Ad. Therefore, E4orf1, perhaps by acting through the PI3-kinase/Akt pathway, limits intrinsic innate and system-wide adaptive immune responses that are important for improved ΔE1B Ad-based vaccines.

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Adenovirus—Simian Virus 40 Interactions

Klessig

1984

The Adenoviruses

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Subgenomic viral DNA species synthesized in simian cells by human and simian adenoviruses

Cited by 8 publications

References 42 publications

Cloning and physical mapping of enteric adenoviruses (candidate types 40 and 41)

Cloning and physical mapping of enteric adenoviruses (candidate types 40 and 41)

E4orf1 Suppresses E1B-Deleted Adenovirus Vaccine-Induced Immune Responses

Adenovirus—Simian Virus 40 Interactions

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