Subgroups of canine antinuclear antibodies in relation to laboratory and clinical findings in immune‐mediated disease

Abstract

In dogs with homogeneous IIF-ANA staining, SLE is a probable diagnosis because of the diversity of clinical manifestations and autoantibody reactivity against chromosomal antigens. Dogs with a speckled IIF-ANA pattern may have SLE-related diseases, which, in turn, may be correlated with different immunodiffusion subgroups. These syndromes had overlapping clinicopathologic features, as described for human patients.

Detection of antinuclear antibodies by the Inno‐Lia ANA update test in canine systemic rheumatic disease

“…According to our own experience, dogs with a homogenous ANA pattern usually have more widespread organ involvement and might have closer clin-ical similarity to human SLE as compared with dogs with a speckled ANA pattern. 3 We can only conclude that, whereas the presence of anti-snRNP and/or anti-Sm autoantibodies have a very strong association to rheumatic conditions in both dogs and humans, it is not possible at present to use fine specificities of autoantibodies against spliceosomal antigens for further diagnostic purposes in dogs.…”

“…Archived serum samples from 20 privately owned canine patients with clinical signs of systemic rheumatic disease (stiffness, pain while moving, intermittent lameness involving multiple limbs, and pain when manipulating joints) and with earlier demonstrated IIF-ANA-positive results [3][4][5][6] were included in this study. The dogs were all positive for IIF-ANA at a dilution of 1:100 or higher, with endpoint titers ranging from 1:100 to 1:25,600; when repeated after at least 4 weeks, the ANA test gave the same positive result.…”

“…1,2 In dogs, the use of human tissue culture cells (human epithelial-2 [HEp-2] cells) as a substrate also makes it possible to divide positive ANA patterns into different subgroups. [3][4][5] In veterinary as well as in human medicine, several of the nuclear antigens that constitute a target for disease-associated autoantibodies have been characterized by molecular biology techniques. Most of these structures have been recognized as nucleic acids, proteins, or nucleic acid-protein complexes, usually having a central biological function.…”

Serum C-reactive protein concentrations in Nova Scotia Duck Tolling Retrievers with immune-mediated rheumatic disease

“…Most, but not all, IMRD cases display antinuclear antibodies (ANA) on indirect immunofluorescence (IIF) [2]. Antinuclear antibodies and non-erosive polyarthritis are also commonly present in the systemic autoimmune inflammatory disease, systemic lupus erythematosus (SLE), in humans as well as in dogs [3–5] and IMRD is considered an SLE-related disorder [2, 6]. …”

Correlação entre achados clínicos e histopatológicos com aqueles da imunofluorescência direta no diagnóstico de lúpus eritematoso discoide canino

“…Nas doenças parasitárias, a exemplo da erliquiose, bartonelose e leishmaniose, aproximadamente, 10 a 20% dos animais podem apresentar títulos positivos ao antígeno nuclear(SMITH et al, 2004).Outras afecções autoimunes, a exemplo da anemia hemolítica e da poliartrite imunemediadas, além da Síndrome de Evans podem, igualmente, gerar títulos positivos no teste sorológico em questão. Infecções bacterianas crônicas, também, podem ocasionar resultado positivo à reação ANA-IFI, uma vez que o DNA bacteriano é considerado um potente estimulador do sistema imune(SMITH et al, 2004;HANSSON- HAMLIN et al, 2006). diferencial entre as duas variantes do lúpus eritematoso.…”